IHB OpenIR  > 期刊论文
Research on the prokaryotic expression and characteristics of VP28, a structure protein of Penaeus monodon white spot syndrome virus
Zhao Xin-yan; Wei Cong; Dai Lin-Fen; Gao Hong; Dai He-ping (hpdai@ihb.ac.cn); Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China
2004
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume28Issue:3Pages:234-239
AbstractWhite Spot Syndrome Virus (WSSV) is an enveloped DNA virus, which causes 100% cumulative mortality of cultured shrimps in 3-4 days. The virus particles contain five major proteins: VP28, VP26, VP24, VP19 and VP15. The mode of entry and systemic infection of WSSV in shrimp and the functions of these virion proteins are not clear. But more and more attention is paid to the function of VP28, which is likely to play a key role in the initial steps of WSSV infection in shrimps. Towards simplifying to study the role of VP28 in the interaction between WSSV and host cells, the gene of VP28 has been cloned into a prokaryotic expression system, induced and expressed for further researching its characteristics. This prokaryoti expression product could not secrete out of the cell membrane, because it is a membrane protein. Rabbit antiserum against prokaryotic expressed VP28 could recognize both prokaryotic expressed VP28 and native WSSV; Mouse antiserum against native WSSV could recognize both WSSV and prokaryotic expressed VP28. A scFv antibody, designated B7. selected from phage display antibody library against WSSV by panning with prokaryotic expressed VP28, could also recognize both prokaryotic expressed VP28 and native WSSV. These data show that the VP28 produced in a prokaryotic expression system has analogous immunogenic characteristics to its native form, although their protein post-modification may differ.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18089
Collection期刊论文
Corresponding AuthorZhao Xin-yan; Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China
Recommended Citation
GB/T 7714
Zhao Xin-yan,Wei Cong,Dai Lin-Fen,et al. Research on the prokaryotic expression and characteristics of VP28, a structure protein of Penaeus monodon white spot syndrome virus[J]. Acta Hydrobiologica Sinica,2004,28(3):234-239.
APA Zhao Xin-yan,Wei Cong,Dai Lin-Fen,Gao Hong,Dai He-ping ,&Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China.(2004).Research on the prokaryotic expression and characteristics of VP28, a structure protein of Penaeus monodon white spot syndrome virus.Acta Hydrobiologica Sinica,28(3),234-239.
MLA Zhao Xin-yan,et al."Research on the prokaryotic expression and characteristics of VP28, a structure protein of Penaeus monodon white spot syndrome virus".Acta Hydrobiologica Sinica 28.3(2004):234-239.
Files in This Item:
File Name/Size DocType Version Access License
Research on the prok(255KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhao Xin-yan]'s Articles
[Wei Cong]'s Articles
[Dai Lin-Fen]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhao Xin-yan]'s Articles
[Wei Cong]'s Articles
[Dai Lin-Fen]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhao Xin-yan]'s Articles
[Wei Cong]'s Articles
[Dai Lin-Fen]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Research on the prokaryotic expression and characteristics of VP28, a structure protein of Penaeus monodon white spot syndrome virus.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.