IHB OpenIR  > 期刊论文
Molecular cloning and evolutionary implications of growth hormone/prolactin family gene cDNAs in grouper Epinephelus coioides
Jia Hai-Bo; Zhou Li; Shi Yao-Hua; Gui Jian-Fang (jfgui@ihb.ac.cn); Gui Jian-Fang; Inst HydrobiolWuhan Ctr Dev BiolState Key Lab Freshwater Ecol and Biotechnol, Chinese Acad Sci, Wuhan, 430072, China
2004
Source PublicationZoological Research
ISSN0254-5853
Volume25Issue:3Pages:242-248
AbstractTotal RNA was isolated from pituitary of grouper (Epinephelus coioides), and SMART cDNA was synthesized from 50 ng RNA. Two genes in the growth hormone/prolactin family were cloned from SMART cDNAs plasmid library. The growth hormone (GH) cDNA contains 938 by and encodes 204 amino acids (aa). The prolactin (PRL) cDNA contains 1 429 by and encodes 212 art. The deduced as sequences of grouper GH, PRL and SL were compared with those of other eight species. Average genetic distances and a dendrogram showing the hierarchical structure of affinity among different nine fishes were implemented in Mega 2 and CLUSTAL W1.64b. Branch and bound strap analysis (1000 replicates) were performed and the degree of support for particular nodes were produced. The three phylogenetic trees drawn from the whole as sequence of GH, PRL and SL were almost identical, Epinephelus coioides, Perca flavescens, Spares aurata and Paralichthys olivaceus were clustered into one group, Oncorhynchus keta and Oncorhynchus mykiss were clustered into one group, Carassius auras and Ictalurus punctatus were clustered into one group, while Anguilla japonica alone was clustered into another group. Alignment of the deduced as sequence and the phylogenetic tree based on GH/PRL/SL showed the same clustering as the present hierarchy of fish and a higher conservation of SL than GH and PRL among various fish species is suggested in this text. Although there is only 24% - 31% identity between the three hormones overall, the C-terminal region shows a higher identity. Grouper SL appears to be more closely related to GH (30.8%) than to PRL (25.6%), which is slightly more than the identity of GH to PRL (24.1%).
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18083
Collection期刊论文
Corresponding AuthorGui Jian-Fang; Inst HydrobiolWuhan Ctr Dev BiolState Key Lab Freshwater Ecol and Biotechnol, Chinese Acad Sci, Wuhan, 430072, China
Recommended Citation
GB/T 7714
Jia Hai-Bo,Zhou Li,Shi Yao-Hua,et al. Molecular cloning and evolutionary implications of growth hormone/prolactin family gene cDNAs in grouper Epinephelus coioides[J]. Zoological Research,2004,25(3):242-248.
APA Jia Hai-Bo,Zhou Li,Shi Yao-Hua,Gui Jian-Fang ,Gui Jian-Fang,&Inst HydrobiolWuhan Ctr Dev BiolState Key Lab Freshwater Ecol and Biotechnol, Chinese Acad Sci, Wuhan, 430072, China.(2004).Molecular cloning and evolutionary implications of growth hormone/prolactin family gene cDNAs in grouper Epinephelus coioides.Zoological Research,25(3),242-248.
MLA Jia Hai-Bo,et al."Molecular cloning and evolutionary implications of growth hormone/prolactin family gene cDNAs in grouper Epinephelus coioides".Zoological Research 25.3(2004):242-248.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Jia Hai-Bo]'s Articles
[Zhou Li]'s Articles
[Shi Yao-Hua]'s Articles
Baidu academic
Similar articles in Baidu academic
[Jia Hai-Bo]'s Articles
[Zhou Li]'s Articles
[Shi Yao-Hua]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Jia Hai-Bo]'s Articles
[Zhou Li]'s Articles
[Shi Yao-Hua]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.