The fecC gene encoding a putative iron (III) dicitrate transporter was cloned from nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, and inactivated. The mutant grows normally in medium with NO3-, NH4+ or without combined nitrogen. But in iron-deficient medium, the mutant grows slowly. Photosynthetic properties were compared between the mutant and the wild type strain, the content of photosynthetic pigments in the mutant is lower than that of the wild-type. The results of RT-PCR experiments show that the fecC gene is expressed under iron-deficient conditions, but is not expressed under iron-replete conditions. These results revealed that fecC gene product is required for optimal growth under iron-deficient conditions in Anabaena sp. PCC 7120.
Xu Wen-liang (firstname.lastname@example.org) ; Liu Yong-ding (email@example.com) ; Zhang Cheng-Cai; Li Juan.Cloning and characterization of the fecC gene necessary for optimal growth under iron-deficiency conditions in the cyanobacterium Anabaena sp. PCC 7120,Wuhan University Journal of Natural Sciences ,2004,9(4):498-502