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Cloning and characterization of the fecC gene necessary for optimal growth under iron-deficiency conditions in the cyanobacterium Anabaena sp. PCC 7120
Xu Wen-liang (wenliangxu@yahoo.com.cn); Liu Yong-ding (liuyd@ihb.ac.cn); Zhang Cheng-Cai; Li Juan; Liu Yong-ding; Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China
2004
Source PublicationWuhan University Journal of Natural Sciences
ISSN1007-1202
Volume9Issue:4Pages:498-502
AbstractThe fecC gene encoding a putative iron (III) dicitrate transporter was cloned from nitrogen-fixing cyanobacterium Anabaena sp. PCC 7120, and inactivated. The mutant grows normally in medium with NO3-, NH4+ or without combined nitrogen. But in iron-deficient medium, the mutant grows slowly. Photosynthetic properties were compared between the mutant and the wild type strain, the content of photosynthetic pigments in the mutant is lower than that of the wild-type. The results of RT-PCR experiments show that the fecC gene is expressed under iron-deficient conditions, but is not expressed under iron-replete conditions. These results revealed that fecC gene product is required for optimal growth under iron-deficient conditions in Anabaena sp. PCC 7120.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18067
Collection期刊论文
Corresponding AuthorLiu Yong-ding; Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China
Recommended Citation
GB/T 7714
Xu Wen-liang ,Liu Yong-ding ,Zhang Cheng-Cai,et al. Cloning and characterization of the fecC gene necessary for optimal growth under iron-deficiency conditions in the cyanobacterium Anabaena sp. PCC 7120[J]. Wuhan University Journal of Natural Sciences,2004,9(4):498-502.
APA Xu Wen-liang ,Liu Yong-ding ,Zhang Cheng-Cai,Li Juan,Liu Yong-ding,&Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China.(2004).Cloning and characterization of the fecC gene necessary for optimal growth under iron-deficiency conditions in the cyanobacterium Anabaena sp. PCC 7120.Wuhan University Journal of Natural Sciences,9(4),498-502.
MLA Xu Wen-liang ,et al."Cloning and characterization of the fecC gene necessary for optimal growth under iron-deficiency conditions in the cyanobacterium Anabaena sp. PCC 7120".Wuhan University Journal of Natural Sciences 9.4(2004):498-502.
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