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Applicability of cetacean microsatellite primers in the Yangtze finless porpoise
Xia Jun-Hong; Zheng Jin-Song; Wang Ding (wangd@ihb.ac.cn); Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China
2004
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume28Issue:6Pages:640-646
AbstractApplication of microsatellites in the Yangtze finless porpoise ( Neophocaena phocaenoides asiaeorientalis) has not been reported. In this study amplification of microsatellites were performed in DNA samples of the Yangtze finless porpoise population by PCR using twenty-three published microsatellite primers from Phocaena phocoena, Eubalaena glacialis, Physeter catodon, Megaptera novaeangliae, Tursiops truncates, Globicephala melas. The results indicated seven primer pairs produced stable and polymorphic PCR products in the population. Further sequencing analysis showed that the amplified product sequences of the seven primer pairs all own AC or GT di-nucleotide repeats, which confirmed that the amplification was valid. The results suggest that applicable primers in fingerprinting analysis of the Yangtze finless porpoise could be obtained faster by screening of microsatellite primers isolated from other cetacean species.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/18047
Collection期刊论文
Corresponding AuthorXia Jun-Hong; Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China
Recommended Citation
GB/T 7714
Xia Jun-Hong,Zheng Jin-Song,Wang Ding ,et al. Applicability of cetacean microsatellite primers in the Yangtze finless porpoise[J]. Acta Hydrobiologica Sinica,2004,28(6):640-646.
APA Xia Jun-Hong,Zheng Jin-Song,Wang Ding ,&Inst Hydrobiol, Chinese Acad Sci, Wuhan, 430072, China.(2004).Applicability of cetacean microsatellite primers in the Yangtze finless porpoise.Acta Hydrobiologica Sinica,28(6),640-646.
MLA Xia Jun-Hong,et al."Applicability of cetacean microsatellite primers in the Yangtze finless porpoise".Acta Hydrobiologica Sinica 28.6(2004):640-646.
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