中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: Structure analysis of thymidylate synthase gene from LCDV-C
作者: Zhao Zhe ; Zhang Qi-ya
通讯作者: Zhang Qi-ya ; Inst HydrobiolState Key Lab Freshwater Ecol and Biotechnol, Chinese Acad Sci, Wuhan, 430072, China
刊名: Virologica Sinica
发表日期: 2004
卷: 19, 期:6, 页:602-606
收录类别: 其他
英文摘要: Thymidylate Synthase (TS) is an enzyme involved in making one of the major building blocks for DNA deoxythymidylic acid (dTMP), and plays a critical role in cell growth and division and is overexpressed in the majority of cancers. Lymphocystis disease virus (LCDV) belong to iridoviridae, and the causative agent of lymphocystis disease, which has been reported to occur in over 100 different fish species worldwide, and viral infection that causes cells to become megaloblastic, thus forming small tumors (bumps or growths). The complete nucleotide sequence of the LCDV-C genome was recently determined. Among the sequenced vertebrate iridoviruses, lymphocystis disease virus isolated from China (LCDV-C) is of the largest genome. Computer-assisted analysis revealed the potential open reading frames ORF 011L of LCDV-C was TS gene, the first report of complete TS gene from vertebrate iridoviruses. The LCDV-C TS gene consists of 858 by in length with a base composition of 28.2% G+C, and encoded a protein of 286 amino acids with a predicted size of 32.7KD. Comparison of the LCDV-C TS to TS from Chilo iridescent virus(CIV), Ambystoma tigrinum virus (ATV) and tiger frog virus (TFV) three iridoviruses and other sixteen species, showed extensive amino acid similarities of TS between four iridoviruses and other species. The predicted LCDV-C TS amino acid sequences contain the folate-binding site in 44-70 residues and dUMP-binding site in 163-206 residues. The secondary structure of LCDV-C TS was predicted with 8 alpha helixes, 6 beta sheets and 23 loops, showed the structure flexible and torsional rotation. Phylogenetic tree was constructed based on the multiple alignments of TS amino acid sequences from twenty species, and LCDV-C TS is a branch alone.
ISSN号: 1003-5125
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/18037
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Structure analysis of thymidylate synthase gene from LCDV-C.pdf(242KB)----开放获取--View Download

Recommended Citation:
Zhao Zhe; Zhang Qi-ya .Structure analysis of thymidylate synthase gene from LCDV-C,Virologica Sinica,2004,19(6):602-606
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao Zhe]'s Articles
[Zhang Qi-ya]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao Zhe]‘s Articles
[Zhang Qi-ya]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Structure analysis of thymidylate synthase gene from LCDV-C.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace