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题名: Cryopreservation of decapsulated Artemia sp. embryos
作者: Liu Xiangyu, Lu Renhou
通讯作者: Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 420072, China
刊名: Acta Hydrobiologica Sinica
发表日期: 1996
卷: 20, 期:3, 页:248-256
收录类别: 其他
英文摘要: The decapsulated Artemia sp. embryos at their blastula stage were successfully cryopreserved in liquid nitrogen with various concentrations of glycerol, DMSO, glucose and sucrose as single cryoprotectants. Higher cryoprotectant concentrations were better than the lowers Dual cryoprotectants (glycerol+DMSO, glucose+sucrose) had stronger cryoprotection than single-type cryoprotection. Saturated NaCl solution showed a powerful cryoprotection for various developmental stages of Artemia sp. embryos, although a weaker cryoprotection was observed for later stage. Intraembryoic ice crystal formation was the main reason causing the death of the embryos. The cryoprotection of various cryoprotectants is possibly related to their dehydration effect.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17987
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Recommended Citation:
Liu Xiangyu, Lu Renhou.Cryopreservation of decapsulated Artemia sp. embryos,Acta Hydrobiologica Sinica,1996,20(3):248-256
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