IHB OpenIR  > 期刊论文
Cryopreservation of decapsulated Artemia sp. embryos
Liu Xiangyu, Lu Renhou; Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 420072, China
1996
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume20Issue:3Pages:248-256
AbstractThe decapsulated Artemia sp. embryos at their blastula stage were successfully cryopreserved in liquid nitrogen with various concentrations of glycerol, DMSO, glucose and sucrose as single cryoprotectants. Higher cryoprotectant concentrations were better than the lowers Dual cryoprotectants (glycerol+DMSO, glucose+sucrose) had stronger cryoprotection than single-type cryoprotection. Saturated NaCl solution showed a powerful cryoprotection for various developmental stages of Artemia sp. embryos, although a weaker cryoprotection was observed for later stage. Intraembryoic ice crystal formation was the main reason causing the death of the embryos. The cryoprotection of various cryoprotectants is possibly related to their dehydration effect.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17987
Collection期刊论文
Corresponding AuthorInst. Hydrobiol., Chinese Acad. Sci., Wuhan 420072, China
Recommended Citation
GB/T 7714
Liu Xiangyu, Lu Renhou,Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 420072, China. Cryopreservation of decapsulated Artemia sp. embryos[J]. Acta Hydrobiologica Sinica,1996,20(3):248-256.
APA Liu Xiangyu, Lu Renhou,&Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 420072, China.(1996).Cryopreservation of decapsulated Artemia sp. embryos.Acta Hydrobiologica Sinica,20(3),248-256.
MLA Liu Xiangyu, Lu Renhou,et al."Cryopreservation of decapsulated Artemia sp. embryos".Acta Hydrobiologica Sinica 20.3(1996):248-256.
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