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题名: Introduction of foreign gene carried by sperms
作者: Li Guohua, Cui Zongbin ; Zhu Zuoyan ; Huang Shanxian
通讯作者: Li Guohua, Cui Zongbin ; Huang Shanxian ; Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
刊名: Acta Hydrobiologica Sinica
发表日期: 1996
卷: 20, 期:3, 页:242-247
收录类别: 其他
英文摘要: Mature eggs from carp (Cyprinus carpio L.) and loach (Misgurnus anguillicaudatus Cantor) were inseminated with the same species sperms which had been incubated with recombinant plasmid DNA containing human GH.gene in a solution containing 2.9% glucose, 1% sodium citrate, 0.2% sodium bicarbonate, 0.03% potassium chloride and 30mg/ml bovine albumin. Individuals of 33.3% carp fry and 37.0% loach fry showed to carry the foreign gene with 4-150 copies per cell by dot blot hybridization and PCR analysis. The studies indicated that sperm could be a vector to carry and introduce foreign gene into fish eggs in the course of fertilization.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17985
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Li Guohua, Cui Zongbin; Zhu Zuoyan; Huang Shanxian.Introduction of foreign gene carried by sperms,Acta Hydrobiologica Sinica,1996,20(3):242-247
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