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Introduction of foreign gene carried by sperms
Li Guohua, Cui Zongbin; Zhu Zuoyan; Huang Shanxian; Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
1996
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume20Issue:3Pages:242-247
AbstractMature eggs from carp (Cyprinus carpio L.) and loach (Misgurnus anguillicaudatus Cantor) were inseminated with the same species sperms which had been incubated with recombinant plasmid DNA containing human GH.gene in a solution containing 2.9% glucose, 1% sodium citrate, 0.2% sodium bicarbonate, 0.03% potassium chloride and 30mg/ml bovine albumin. Individuals of 33.3% carp fry and 37.0% loach fry showed to carry the foreign gene with 4-150 copies per cell by dot blot hybridization and PCR analysis. The studies indicated that sperm could be a vector to carry and introduce foreign gene into fish eggs in the course of fertilization.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17985
Collection期刊论文
Corresponding AuthorLi Guohua, Cui Zongbin; Huang Shanxian; Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
Recommended Citation
GB/T 7714
Li Guohua, Cui Zongbin,Zhu Zuoyan,Huang Shanxian,et al. Introduction of foreign gene carried by sperms[J]. Acta Hydrobiologica Sinica,1996,20(3):242-247.
APA Li Guohua, Cui Zongbin,Zhu Zuoyan,Huang Shanxian,&Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China.(1996).Introduction of foreign gene carried by sperms.Acta Hydrobiologica Sinica,20(3),242-247.
MLA Li Guohua, Cui Zongbin,et al."Introduction of foreign gene carried by sperms".Acta Hydrobiologica Sinica 20.3(1996):242-247.
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