This paper presents some results of an investigation on fish community composition and diversity in five lake areas, situated to the middle reach of Yangtze River, and in Hubei Province. There are 46 species, 42 species, 45 species, 42 species, and 45 species of fish, respectively, in north region of Qinglinghu Lake, south region of it, Huangjiahu Lake, Qiaodenhu region of, and Biandantang region of Bao'an Lake. Of the lake areas, fish species diversity and equitability in Biandantang region are the highest, while in the north region, are the lowest; Inversely, species concentration in the former is the smallest, while is the biggest in the latter. Moreover, Coefficient of similarity of two fish communities among the five lake areas is measured. The results of this paper preliminary demonstrate that the artificial propagation may result in negative effect on fish species diversity in five lake areas, and that fish species diversity in macrophytic lakes is higher tendency as compared with that of algae lakes.