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Isolation and purification of hirudin from a blood-sucking leech, Hirudo nipponia, in China
Yang Tong, Li Jianqiu; Yin Ping; Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
1997
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume21Issue:2Pages:169-173
AbstractThis paper deals with the isolation and purification of hirudin from a bloodsucking leech, Hirudo nipponia in China. Essentially, the experimental process requires an anion exchange chromatography on DEAE-C-52 and a gel filtration chromatography on Sephadex-G-25. The hirudin activity were measured by the method of titration with thrombin. The salivary gland secretions have been obtained from the alive leeches. The hirudin content in salivary gland secretions is 4 AT-U/ml; the hirudin production rate is 15%; ratio activity after purifying is 6708 AT-U/ml protein.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17887
Collection期刊论文
Corresponding AuthorYang Tong, Li Jianqiu; Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
Recommended Citation
GB/T 7714
Yang Tong, Li Jianqiu,Yin Ping,Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China. Isolation and purification of hirudin from a blood-sucking leech, Hirudo nipponia, in China[J]. Acta Hydrobiologica Sinica,1997,21(2):169-173.
APA Yang Tong, Li Jianqiu,Yin Ping,&Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China.(1997).Isolation and purification of hirudin from a blood-sucking leech, Hirudo nipponia, in China.Acta Hydrobiologica Sinica,21(2),169-173.
MLA Yang Tong, Li Jianqiu,et al."Isolation and purification of hirudin from a blood-sucking leech, Hirudo nipponia, in China".Acta Hydrobiologica Sinica 21.2(1997):169-173.
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