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题名: Isolation and purification of hirudin from a blood-sucking leech, Hirudo nipponia, in China
作者: Yang Tong, Li Jianqiu ; Yin Ping
通讯作者: Yang Tong, Li Jianqiu ; Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
刊名: Acta Hydrobiologica Sinica
发表日期: 1997
卷: 21, 期:2, 页:169-173
收录类别: 其他
英文摘要: This paper deals with the isolation and purification of hirudin from a bloodsucking leech, Hirudo nipponia in China. Essentially, the experimental process requires an anion exchange chromatography on DEAE-C-52 and a gel filtration chromatography on Sephadex-G-25. The hirudin activity were measured by the method of titration with thrombin. The salivary gland secretions have been obtained from the alive leeches. The hirudin content in salivary gland secretions is 4 AT-U/ml; the hirudin production rate is 15%; ratio activity after purifying is 6708 AT-U/ml protein.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17887
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Yang Tong, Li Jianqiu; Yin Ping.Isolation and purification of hirudin from a blood-sucking leech, Hirudo nipponia, in China,Acta Hydrobiologica Sinica,1997,21(2):169-173
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