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题名: Chromosome ploidy manipulation of allotetraploids and their fertility in Japanese phytophagous crucian carp (JPCC) (female) X red crucian carp (RCC) (male)
作者: Chen, Minrong ; Yang, Xingqi ; Yu, Xiaomu ; Chen, Hongxi ; Yi, Yonglan ; Liu, Hanqin
通讯作者: Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
刊名: Acta Hydrobiologica Sinica
发表日期: 1997
卷: 21, 期:3, 页:197-206
收录类别: 其他
英文摘要: Heat shock was used to induce allotetraploidy in Japanese phytophagous crucian carp (JPCC) eggs fertilized artificially with sperms from red crucian carp (RCC) 37-38 min after fertilization and shortly before cleavage. Milt was obtained from 1- and 2-year old h4n JPCC male, but ovulated females were not found, and back crossed to the 2n JPCC female produced a large number of offsprings. Chromosome analysis of the offsprings, based on cultured leucocytes from peripheral blood indicated that all were triploids (3n = 150). The fertilizing ability of, the offspring was very low (11.451.3% of fertility, average: 32.4%). The present observation supports the hypothesis that the head of the diploid spermatozoas being wider than the haploid may prevent their penetration through the micropyle canal. Females or males of interploid triploid JPCC did not develop into maturity, but they have higher growth rate than the diploid one. New tetraploids were produced by the retention of the second polar body following fertilization (4Nmale X 2N female), and they were found to be tetraploid, triploid and diploid by chromosome analysis (among 72 resultant fish, there were 19 tetraploid, 32 triploid and 21 diploid, respectively.)
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17831
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Chen, Minrong; Yang, Xingqi; Yu, Xiaomu; Chen, Hongxi; Yi, Yonglan; Liu, Hanqin.Chromosome ploidy manipulation of allotetraploids and their fertility in Japanese phytophagous crucian carp (JPCC) (female) X red crucian carp (RCC) (male),Acta Hydrobiologica Sinica,1997,21(3):197-206
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文件名: Chromosome ploidy manipulation of allotetraploids and their fertility in Japanese phytophagous crucian carp (JPCC) (female) X red crucian carp (RCC) (male).pdf
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