IHB OpenIR  > 期刊论文
An artificial transformation system of cyanobacteria
Min Hongtao, Wang Yeqin; Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
1995
Source PublicationActa Hydrobiologica Sinica
ISSN1000-3207
Volume19Issue:3Pages:227-233
AbstractArtificial competence of Synechococcus PCC 6301 cells was induced by lysozyme treatment and the cells were transformed to chloramphenicol resistance with foreign plasmid pBR325 at a frequency of approximately 5 times 10-5 or 5 times 10-4 with the transformant DNA. The transformation frequencies were higher than those reported by other workers for the same strain with cloned DNA employing a physiological transformation system. Analyses of DNA electrophoresis, secondary transformation and dot blotting demonstrated that foreign plasmid had integrated into the recipient chromosome by a single crossover event. The results showed that the artificial transformation system was efficient and reproducible. Conditions that affected transformation, such as, incubation time of cells with DNA, age of the cells, light or dark incubation were also studied.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17723
Collection期刊论文
Corresponding AuthorInst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
Recommended Citation
GB/T 7714
Min Hongtao, Wang Yeqin,Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China. An artificial transformation system of cyanobacteria[J]. Acta Hydrobiologica Sinica,1995,19(3):227-233.
APA Min Hongtao, Wang Yeqin,&Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China.(1995).An artificial transformation system of cyanobacteria.Acta Hydrobiologica Sinica,19(3),227-233.
MLA Min Hongtao, Wang Yeqin,et al."An artificial transformation system of cyanobacteria".Acta Hydrobiologica Sinica 19.3(1995):227-233.
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