中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 中科院水生所知识产出(2009年前)  > 期刊论文
题名: An artificial transformation system of cyanobacteria
作者: Min Hongtao, Wang Yeqin
通讯作者: Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
刊名: Acta Hydrobiologica Sinica
发表日期: 1995
卷: 19, 期:3, 页:227-233
收录类别: 其他
英文摘要: Artificial competence of Synechococcus PCC 6301 cells was induced by lysozyme treatment and the cells were transformed to chloramphenicol resistance with foreign plasmid pBR325 at a frequency of approximately 5 times 10-5 or 5 times 10-4 with the transformant DNA. The transformation frequencies were higher than those reported by other workers for the same strain with cloned DNA employing a physiological transformation system. Analyses of DNA electrophoresis, secondary transformation and dot blotting demonstrated that foreign plasmid had integrated into the recipient chromosome by a single crossover event. The results showed that the artificial transformation system was efficient and reproducible. Conditions that affected transformation, such as, incubation time of cells with DNA, age of the cells, light or dark incubation were also studied.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17723
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
An artificial transformation system of cyanobacteria.pdf(381KB)----开放获取--View Download

Recommended Citation:
Min Hongtao, Wang Yeqin.An artificial transformation system of cyanobacteria,Acta Hydrobiologica Sinica,1995,19(3):227-233
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Min Hongtao, Wang Yeqin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Min Hongtao, Wang Yeqin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: An artificial transformation system of cyanobacteria.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace