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题名: Light microscope investigation of meiotic chromosome pairing in artificial triploid fishes with blocked ovaries
作者: Giu Jianfang, Chen Li ; Liang Shaochang ; Jiang Yigui
通讯作者: Giu Jianfang, Chen Li ; Jiang Yigui ; Inst. Hydrobiol., Chinese Acad. Sci., Wuhan 430072, China
刊名: Acta Hydrobiologica Sinica
发表日期: 1995
卷: 19, 期:3, 页:223-226
收录类别: 其他
英文摘要: Chromosome pairing was light microscopically investigated by applying surface spreading technique in combination with 1-step silver staining method for the pachytene chromosome of artificial triploid transparent colored crucian carp (Carassius auratus transparent colored variety) with blocked ovaries. The triploid fishes were produced from hydrostatic pressure shock for fertilized eggs by inducing second polar body retention. The study was performed on the triploid females with blocked ovaries, one of many different types of females with various developing ovaries. Two subtypes were distinguished according to the presence or absence of primary oocytes. Meiotic prophase cells were not observed in the triploid females without primary oocytes. In the triploid females with blocked ovaries containing differentiated primary oocytes, the pachytene chromosomes were mainly composed of bivalents and unsynapsed univalents, and a few of trivalents and other multivalents were also found. There are about 90 chromosome elements including univalents, bivalents, trivalents and other multivalents in the pachytene cells. The size of chromosomes among different pachytene cells was obviously various. The pairing forks and specific synaptonemal proteins were clearly observed at some pairing and paired chromosomes. The relationship between disturbed chromosome pairing and blocked oocyte developing has been briefly discussed. The investigation also suggested that the artificial triploid fishes might be excellent materials for studying the mechanism of chromosome pairing and synapsing.
ISSN号: 1000-3207
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17721
Appears in Collections:中科院水生所知识产出(2009年前)_期刊论文

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Giu Jianfang, Chen Li; Liang Shaochang; Jiang Yigui.Light microscope investigation of meiotic chromosome pairing in artificial triploid fishes with blocked ovaries,Acta Hydrobiologica Sinica,1995,19(3):223-226
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文件名: Light microscope investigation of meiotic chromosome pairing in artificial triploid fishes with blocked ovaries.pdf
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