Six species of Anabaena were initially examined for their ability to grow on glucose and sucrose chemoheterotrophically. Among these species, HB1017 grew fast and HB 0 very slow, while the other four species did not survive at all. The effects of fructose, glucose and sucrose, though all supported growth of HB1017, on growth were apparently different. The positive effect of the sugar substrates was in the order of fructose, glucose and sucrose. It was found that autoclaved fructose and sucrose only supported a much lower heterotrophic growth rate than the filtrated ones, while autoclaved glucose sustained a higher growth rate as did the filtrated fructose. The addition of exogenous arginine increased heterotrophic growth rate, and NaNO-3 was suitable nitrogen source for growth. It appears that optimal growth was obtained if the culture was provided with appropriate stirring and temperature at 35 degree C. The photoautotrophic growth culture of HB1017, when transfered to chemoheterotrophic condition, could quickly start to grow in it, without the "adaptation phase". The average heterotrophic growth rate for 3 days was 0.74, and the generation time was 0.9 day.