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PURIFICATION AND SOME PROPERTIES OF IRON PROTEIN OF NITROGENASE FROM ANABAENA-CYLINDRICA
DU D; LIN H; HE Z; DAI L; XIN W; LI S; INST HYDROBIOL, ACAD SINICA, WUHAN
1990
Source PublicationChinese Journal of Oceanology and Limnology
ISSN0254-4059
Volume8Issue:4Pages:378-384
AbstractThe Fe protein of Anabaena cylindrica was first separated and purified by chromatography through DEAE-cellulose columns then by gel electrophoresis. The specific activity was up to 142.46 nmol C2H4/mg protein .cntdot. min. It was homogeneous as shown by (1) a single band in the gel electrophorogram; (2) absence of Mo and tryptophan; (3) content of about 3.4 atoms of Fe per mole protein. The molecular weight of the Fe protein of A. cylindrica was about 61,000 daltons as estimated by SDS-gel electrophoresis and calculated from the amino acid composition. The residues of aspartate and glutamate were about 2.6 times that of arginine and lysine in the Fe protein. Crossing Fe protein of A. cylindrica with Mo-Fe protein of Azotobacter vinelandii gave positive result. The reciprocal crossing also showed activity.
Indexed By其他
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17673
Collection期刊论文
Corresponding AuthorDU D; INST HYDROBIOL, ACAD SINICA, WUHAN
Recommended Citation
GB/T 7714
DU D,LIN H,HE Z,et al. PURIFICATION AND SOME PROPERTIES OF IRON PROTEIN OF NITROGENASE FROM ANABAENA-CYLINDRICA[J]. Chinese Journal of Oceanology and Limnology,1990,8(4):378-384.
APA DU D.,LIN H.,HE Z.,DAI L.,XIN W.,...&INST HYDROBIOL, ACAD SINICA, WUHAN.(1990).PURIFICATION AND SOME PROPERTIES OF IRON PROTEIN OF NITROGENASE FROM ANABAENA-CYLINDRICA.Chinese Journal of Oceanology and Limnology,8(4),378-384.
MLA DU D,et al."PURIFICATION AND SOME PROPERTIES OF IRON PROTEIN OF NITROGENASE FROM ANABAENA-CYLINDRICA".Chinese Journal of Oceanology and Limnology 8.4(1990):378-384.
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