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题名: A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters
作者: Zha, Shihong1; Xu, Xudong1; Hu, Hanhua1
通讯作者: Hu, HH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Nostoc sp ; PCC 7120 ; bioreporter ; iron ; alr0397
刊名: FEMS MICROBIOLOGY LETTERS
发表日期: 2012-09-01
DOI: 10.1111/j.1574-6968.2012.02629.x
卷: 334, 期:2, 页:135-142
收录类别: SCI
文章类型: Article
部门归属: [Zha, Shihong; Xu, Xudong; Hu, Hanhua] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: A Nostoc sp. PCC 7120 iron bioreporter containing iron-regulated schizokinen transporter gene alr0397 promoter fused to the luxAB genes was examined to optimize its response to bioavailable iron. Doseresponse relationships between luciferase activity and free ferric ion (Fe3+) concentrations pFe (-lg [Fe3+]) were generated by measuring luciferase activities of the bioreporter in trace metalbuffered Fraquil medium with various incubation times. The results were best demonstrated by sigmoidal curves (pFe 18.821.7, Fe3+ = 10-18.810-21.7 M) with the linear range extending from pFe 19.621.5 (Fe3+ = 10-19.610-21.5 M) after a 12-h incubation time. Optimal conditions for the use of this bioreporter to sense the iron bioavailability were determined to be: a 12-h exposure time, initial cell density of OD730 nm = 0.06, high nitrate (100 mu M), high phosphate (10 mu M), moderate Co2+ (0.122.5 nM), Zn2+ (0.1612 nM), Cu2+ (0.0450 nM), and wide range of Mn2+ concentration (0.922300 nM). The applicability of using this iron bioreporter to assess iron availability in the natural environment has been tested using water samples from eutrophic Taihu, Donghu, and Chaohu lakes. It is indicated that the bioreporter is a useful tool to assess bioavailable iron in various water quality samples, especially in eutrophic lakes with high bioavailable iron.
英文摘要: A Nostoc sp. PCC 7120 iron bioreporter containing iron-regulated schizokinen transporter gene alr0397 promoter fused to the luxAB genes was examined to optimize its response to bioavailable iron. Doseresponse relationships between luciferase activity and free ferric ion (Fe3+) concentrations pFe (-lg [Fe3+]) were generated by measuring luciferase activities of the bioreporter in trace metalbuffered Fraquil medium with various incubation times. The results were best demonstrated by sigmoidal curves (pFe 18.821.7, Fe3+ = 10-18.810-21.7 M) with the linear range extending from pFe 19.621.5 (Fe3+ = 10-19.610-21.5 M) after a 12-h incubation time. Optimal conditions for the use of this bioreporter to sense the iron bioavailability were determined to be: a 12-h exposure time, initial cell density of OD730 nm = 0.06, high nitrate (100 mu M), high phosphate (10 mu M), moderate Co2+ (0.122.5 nM), Zn2+ (0.1612 nM), Cu2+ (0.0450 nM), and wide range of Mn2+ concentration (0.922300 nM). The applicability of using this iron bioreporter to assess iron availability in the natural environment has been tested using water samples from eutrophic Taihu, Donghu, and Chaohu lakes. It is indicated that the bioreporter is a useful tool to assess bioavailable iron in various water quality samples, especially in eutrophic lakes with high bioavailable iron.
关键词[WOS]: STRAIN PCC 7120 ; LAKE-ERIE ; CYANOBACTERIAL BIOREPORTER ; MARINE-PHYTOPLANKTON ; COMPLEXING LIGANDS ; ESCHERICHIA-COLI ; FE ; SYNECHOCOCCUS ; ANABAENA ; PHOSPHORUS
语种: 英语
WOS记录号: WOS:000307888400009
ISSN号: 0378-1097
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17294
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

Recommended Citation:
Zha, Shihong; Xu, Xudong; Hu, Hanhua.A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters,FEMS MICROBIOLOGY LETTERS,2012,334(2):135-142
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