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A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters
Zha, Shihong; Xu, Xudong; Hu, Hanhua; Hu, HH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2012-09-01
Source PublicationFEMS MICROBIOLOGY LETTERS
ISSN0378-1097
Volume334Issue:2Pages:135-142
AbstractA Nostoc sp. PCC 7120 iron bioreporter containing iron-regulated schizokinen transporter gene alr0397 promoter fused to the luxAB genes was examined to optimize its response to bioavailable iron. Doseresponse relationships between luciferase activity and free ferric ion (Fe3+) concentrations pFe (-lg [Fe3+]) were generated by measuring luciferase activities of the bioreporter in trace metalbuffered Fraquil medium with various incubation times. The results were best demonstrated by sigmoidal curves (pFe 18.821.7, Fe3+ = 10-18.810-21.7 M) with the linear range extending from pFe 19.621.5 (Fe3+ = 10-19.610-21.5 M) after a 12-h incubation time. Optimal conditions for the use of this bioreporter to sense the iron bioavailability were determined to be: a 12-h exposure time, initial cell density of OD730 nm = 0.06, high nitrate (100 mu M), high phosphate (10 mu M), moderate Co2+ (0.122.5 nM), Zn2+ (0.1612 nM), Cu2+ (0.0450 nM), and wide range of Mn2+ concentration (0.922300 nM). The applicability of using this iron bioreporter to assess iron availability in the natural environment has been tested using water samples from eutrophic Taihu, Donghu, and Chaohu lakes. It is indicated that the bioreporter is a useful tool to assess bioavailable iron in various water quality samples, especially in eutrophic lakes with high bioavailable iron.; A Nostoc sp. PCC 7120 iron bioreporter containing iron-regulated schizokinen transporter gene alr0397 promoter fused to the luxAB genes was examined to optimize its response to bioavailable iron. Doseresponse relationships between luciferase activity and free ferric ion (Fe3+) concentrations pFe (-lg [Fe3+]) were generated by measuring luciferase activities of the bioreporter in trace metalbuffered Fraquil medium with various incubation times. The results were best demonstrated by sigmoidal curves (pFe 18.821.7, Fe3+ = 10-18.810-21.7 M) with the linear range extending from pFe 19.621.5 (Fe3+ = 10-19.610-21.5 M) after a 12-h incubation time. Optimal conditions for the use of this bioreporter to sense the iron bioavailability were determined to be: a 12-h exposure time, initial cell density of OD730 nm = 0.06, high nitrate (100 mu M), high phosphate (10 mu M), moderate Co2+ (0.122.5 nM), Zn2+ (0.1612 nM), Cu2+ (0.0450 nM), and wide range of Mn2+ concentration (0.922300 nM). The applicability of using this iron bioreporter to assess iron availability in the natural environment has been tested using water samples from eutrophic Taihu, Donghu, and Chaohu lakes. It is indicated that the bioreporter is a useful tool to assess bioavailable iron in various water quality samples, especially in eutrophic lakes with high bioavailable iron.
SubtypeArticle
KeywordNostoc Sp Pcc 7120 Bioreporter Iron Alr0397
Department[Zha, Shihong; Xu, Xudong; Hu, Hanhua] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
DOI10.1111/j.1574-6968.2012.02629.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000307888400009
WOS KeywordSTRAIN PCC 7120 ; LAKE-ERIE ; CYANOBACTERIAL BIOREPORTER ; MARINE-PHYTOPLANKTON ; COMPLEXING LIGANDS ; ESCHERICHIA-COLI ; FE ; SYNECHOCOCCUS ; ANABAENA ; PHOSPHORUS
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17294
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHu, HH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Zha, Shihong,Xu, Xudong,Hu, Hanhua,et al. A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters[J]. FEMS MICROBIOLOGY LETTERS,2012,334(2):135-142.
APA Zha, Shihong,Xu, Xudong,Hu, Hanhua,&Hu, HH .(2012).A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters.FEMS MICROBIOLOGY LETTERS,334(2),135-142.
MLA Zha, Shihong,et al."A high sensitivity iron-dependent bioreporter used to measure iron bioavailability in freshwaters".FEMS MICROBIOLOGY LETTERS 334.2(2012):135-142.
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