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题名: RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803
作者: Majeed, Waqar1; Zhang, Yan2; Xue, Yong1; Ranade, Saurabh1; Blue, Ryan Nastashia1; Wang, Qiang3; He, Qingfang1, 2
通讯作者: Majeed, W (reprint author), Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA.
关键词: SP STRAIN PCC-6803 ; CYCLIC ADP-RIBOSE ; PHYSIOLOGICAL SIGNIFICANCE ; INDUCIBLE POLYPEPTIDES ; STATE TRANSITIONS ; GENE-EXPRESSION ; STOICHIOMETRY ; CYANOBACTERIA ; PHOTOPROTECTION ; PHOTOINHIBITION
刊名: PLOS ONE
发表日期: 2012-09-14
DOI: 10.1371/journal.pone.0045139
卷: 7, 期:9, 页:e45139
收录类别: SCI
文章类型: Article
部门归属: [Majeed, Waqar; Xue, Yong; Ranade, Saurabh; Blue, Ryan Nastashia; He, Qingfang] Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA; [Zhang, Yan; He, Qingfang] Shandong Acad Agr Sci, High Tech Res Ctr, Jinan, Shandong, Peoples R China; [Wang, Qiang] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China
WOS标题词: Science & Technology
资助者: National Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: The response regulator RpaA was examined by targeted mutagenesis under high light conditions in Synechocystis sp. PCC 6803. A significant reduction in chlorophyll fluorescence from photosystem I at 77 K was observed in the RpaA mutant cells under high light conditions. Interestingly, the chlorophyll fluorescence emission from the photosystem I trimers at 77 K are similar to that of the wild type, while the chlorophyll fluorescence from the photosystem I monomers was at a much lower level in the mutant than in the wild type under high light conditions. The RpaA inactivation resulted in a dramatic reduction in the monomeric photosystem I and the D1 protein but not the CP47 content. However, there is no significant difference in the transcript levels of psaA or psbA or other genes examined, most of which are involved in photosynthesis, pigment biosynthesis, or stress responses. Under high light conditions, the growth of the mutant was affected, and both the chlorophyll content and the whole-chain oxygen evolution capability of the mutant were found to be significantly lower than those of the wild type, respectively. We propose that RpaA regulates the accumulation of the monomeric photosystem I and the D1 protein under high light conditions. This is the first report demonstrating that inactivation of a stress response regulator has specifically reduced the monomeric photosystem I. It suggests that PS I monomers and PS I trimers can be regulated independently for acclimation of cells to high light stress.
英文摘要: The response regulator RpaA was examined by targeted mutagenesis under high light conditions in Synechocystis sp. PCC 6803. A significant reduction in chlorophyll fluorescence from photosystem I at 77 K was observed in the RpaA mutant cells under high light conditions. Interestingly, the chlorophyll fluorescence emission from the photosystem I trimers at 77 K are similar to that of the wild type, while the chlorophyll fluorescence from the photosystem I monomers was at a much lower level in the mutant than in the wild type under high light conditions. The RpaA inactivation resulted in a dramatic reduction in the monomeric photosystem I and the D1 protein but not the CP47 content. However, there is no significant difference in the transcript levels of psaA or psbA or other genes examined, most of which are involved in photosynthesis, pigment biosynthesis, or stress responses. Under high light conditions, the growth of the mutant was affected, and both the chlorophyll content and the whole-chain oxygen evolution capability of the mutant were found to be significantly lower than those of the wild type, respectively. We propose that RpaA regulates the accumulation of the monomeric photosystem I and the D1 protein under high light conditions. This is the first report demonstrating that inactivation of a stress response regulator has specifically reduced the monomeric photosystem I. It suggests that PS I monomers and PS I trimers can be regulated independently for acclimation of cells to high light stress.
关键词[WOS]: SP STRAIN PCC-6803 ; CYCLIC ADP-RIBOSE ; PHYSIOLOGICAL SIGNIFICANCE ; INDUCIBLE POLYPEPTIDES ; STATE TRANSITIONS ; GENE-EXPRESSION ; STOICHIOMETRY ; CYANOBACTERIA ; PHOTOPROTECTION ; PHOTOINHIBITION
语种: 英语
WOS记录号: WOS:000308860100060
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17290
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA
2.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China

Recommended Citation:
Majeed, Waqar; Zhang, Yan; Xue, Yong; Ranade, Saurabh; Blue, Ryan Nastashia; Wang, Qiang; He, Qingfang.RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803,PLOS ONE,2012,7(9):e45139
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