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RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803
Majeed, Waqar1; Zhang, Yan2; Xue, Yong1; Ranade, Saurabh1; Blue, Ryan Nastashia1; Wang, Qiang3; He, Qingfang1,2; Majeed, W (reprint author), Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA.
2012-09-14
Source PublicationPLOS ONE
ISSN1932-6203
Volume7Issue:9Pages:e45139
AbstractThe response regulator RpaA was examined by targeted mutagenesis under high light conditions in Synechocystis sp. PCC 6803. A significant reduction in chlorophyll fluorescence from photosystem I at 77 K was observed in the RpaA mutant cells under high light conditions. Interestingly, the chlorophyll fluorescence emission from the photosystem I trimers at 77 K are similar to that of the wild type, while the chlorophyll fluorescence from the photosystem I monomers was at a much lower level in the mutant than in the wild type under high light conditions. The RpaA inactivation resulted in a dramatic reduction in the monomeric photosystem I and the D1 protein but not the CP47 content. However, there is no significant difference in the transcript levels of psaA or psbA or other genes examined, most of which are involved in photosynthesis, pigment biosynthesis, or stress responses. Under high light conditions, the growth of the mutant was affected, and both the chlorophyll content and the whole-chain oxygen evolution capability of the mutant were found to be significantly lower than those of the wild type, respectively. We propose that RpaA regulates the accumulation of the monomeric photosystem I and the D1 protein under high light conditions. This is the first report demonstrating that inactivation of a stress response regulator has specifically reduced the monomeric photosystem I. It suggests that PS I monomers and PS I trimers can be regulated independently for acclimation of cells to high light stress.; The response regulator RpaA was examined by targeted mutagenesis under high light conditions in Synechocystis sp. PCC 6803. A significant reduction in chlorophyll fluorescence from photosystem I at 77 K was observed in the RpaA mutant cells under high light conditions. Interestingly, the chlorophyll fluorescence emission from the photosystem I trimers at 77 K are similar to that of the wild type, while the chlorophyll fluorescence from the photosystem I monomers was at a much lower level in the mutant than in the wild type under high light conditions. The RpaA inactivation resulted in a dramatic reduction in the monomeric photosystem I and the D1 protein but not the CP47 content. However, there is no significant difference in the transcript levels of psaA or psbA or other genes examined, most of which are involved in photosynthesis, pigment biosynthesis, or stress responses. Under high light conditions, the growth of the mutant was affected, and both the chlorophyll content and the whole-chain oxygen evolution capability of the mutant were found to be significantly lower than those of the wild type, respectively. We propose that RpaA regulates the accumulation of the monomeric photosystem I and the D1 protein under high light conditions. This is the first report demonstrating that inactivation of a stress response regulator has specifically reduced the monomeric photosystem I. It suggests that PS I monomers and PS I trimers can be regulated independently for acclimation of cells to high light stress.
SubtypeArticle
KeywordSp Strain Pcc-6803 Cyclic Adp-ribose Physiological Significance Inducible Polypeptides State Transitions Gene-expression Stoichiometry Cyanobacteria Photoprotection Photoinhibition
Department[Majeed, Waqar; Xue, Yong; Ranade, Saurabh; Blue, Ryan Nastashia; He, Qingfang] Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA; [Zhang, Yan; He, Qingfang] Shandong Acad Agr Sci, High Tech Res Ctr, Jinan, Shandong, Peoples R China; [Wang, Qiang] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China
DOI10.1371/journal.pone.0045139
WOS HeadingsScience & Technology
Funding OrganizationNational Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902] ; National Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902] ; National Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902] ; National Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000308860100060
WOS KeywordSP STRAIN PCC-6803 ; CYCLIC ADP-RIBOSE ; PHYSIOLOGICAL SIGNIFICANCE ; INDUCIBLE POLYPEPTIDES ; STATE TRANSITIONS ; GENE-EXPRESSION ; STOICHIOMETRY ; CYANOBACTERIA ; PHOTOPROTECTION ; PHOTOINHIBITION
Funding OrganizationNational Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902] ; National Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902] ; National Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902] ; National Science Foundation [MCB 1120153]; Program on Key Basic Research Project [2011CB200902]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17290
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorMajeed, W (reprint author), Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA.
Affiliation1.Univ Arkansas, Dept Appl Sci, Little Rock, AR 72204 USA
2.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan, Shandong, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Majeed, Waqar,Zhang, Yan,Xue, Yong,et al. RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803[J]. PLOS ONE,2012,7(9):e45139.
APA Majeed, Waqar.,Zhang, Yan.,Xue, Yong.,Ranade, Saurabh.,Blue, Ryan Nastashia.,...&Majeed, W .(2012).RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803.PLOS ONE,7(9),e45139.
MLA Majeed, Waqar,et al."RpaA Regulates the Accumulation of Monomeric Photosystem I and PsbA under High Light Conditions in Synechocystis sp PCC 6803".PLOS ONE 7.9(2012):e45139.
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