IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
INFLUENCE OF CLINOROTATION ON CELLULAR STRUCTURE, PHOTOSYNTHETIC ACTIVITY, CARBOHYDRATE AND ASTAXANTHIN METABOLISM OF HAEMATOCOCCUS PLUVIALIS
Zhang, Yu1,2; Li, Xiao-yan1; Ge, Hong-Mei1; Wu, Li1; Xia, Ling1; Wang, Gao-hong1; Hu, Chun-xiang1; Liu, Yong-ding1; Hu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2012
Source PublicationFRESENIUS ENVIRONMENTAL BULLETIN
ISSN1018-4619
Volume21Issue:8Pages:2017-2026
AbstractThe effects of 20 days clinorotation on cellular structure, photosynthetic activity, and the carbohydrate and astaxanthin metabolism of Haematococcus pluvialis provide insights in algal cell adaption. Light micrographs showed the cell shape appeared abnormal and the volume became smaller than the ground control. Ultrastructure analysis revealed that the starch grains seemed smaller and the thylakoid membranes were more randomly distributed and loosened after 20 days of clinorotation. The decreased photosystem II (PSII) photochemical fluorescence efficiency (Fv/Fm) indicated a slow-down of photosynthetic capacity under clinorotation, most likely related to the chloroplast structure changes. The smaller starch grains, together with the decreased starch content were probably either due to an increased hydrolysis of amylase, or a decreased starch synthesis by ADP-glucose pyrophosphorylase during clinorotation. The increased sucrose and trehalose played a protective role in algal cell's accommodation to early clinorotation. However this led to a decrease of glucose and fructose utilized for the synthesis of protective disaccharides. In the middle phase of clinorotation (i.e. the acclimation period), a compensatory synthesis of chlorophyll, glucose and fructose and a reduction of sucrose and trehalose were observed. From the decreased anabolism of astaxanthin, it could be deduced that the clinorotation environment caused a decreased astaxanthin metabolism in Haematococcus pluvialis.; The effects of 20 days clinorotation on cellular structure, photosynthetic activity, and the carbohydrate and astaxanthin metabolism of Haematococcus pluvialis provide insights in algal cell adaption. Light micrographs showed the cell shape appeared abnormal and the volume became smaller than the ground control. Ultrastructure analysis revealed that the starch grains seemed smaller and the thylakoid membranes were more randomly distributed and loosened after 20 days of clinorotation. The decreased photosystem II (PSII) photochemical fluorescence efficiency (Fv/Fm) indicated a slow-down of photosynthetic capacity under clinorotation, most likely related to the chloroplast structure changes. The smaller starch grains, together with the decreased starch content were probably either due to an increased hydrolysis of amylase, or a decreased starch synthesis by ADP-glucose pyrophosphorylase during clinorotation. The increased sucrose and trehalose played a protective role in algal cell's accommodation to early clinorotation. However this led to a decrease of glucose and fructose utilized for the synthesis of protective disaccharides. In the middle phase of clinorotation (i.e. the acclimation period), a compensatory synthesis of chlorophyll, glucose and fructose and a reduction of sucrose and trehalose were observed. From the decreased anabolism of astaxanthin, it could be deduced that the clinorotation environment caused a decreased astaxanthin metabolism in Haematococcus pluvialis.
SubtypeArticle
KeywordClinorotation Haematococcus Pluvialis Sucrose Trehalose Astaxanthin Carbohydrate Metabolism
Department[Zhang, Yu; Li, Xiao-yan; Ge, Hong-Mei; Wu, Li; Xia, Ling; Wang, Gao-hong; Hu, Chun-xiang; Liu, Yong-ding] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Zhang, Yu] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationStrategic Pilot Project of Chinese Academy of Sciences [XDA04074002] ; Strategic Pilot Project of Chinese Academy of Sciences [XDA04074002] ; Strategic Pilot Project of Chinese Academy of Sciences [XDA04074002] ; Strategic Pilot Project of Chinese Academy of Sciences [XDA04074002]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000309326000003
WOS KeywordELECTRON MICROSCOPY ; GENE-EXPRESSION ; TREHALOSE ; MICROGRAVITY ; GROWTH ; RESPONSES ; ACCUMULATION ; DESICCATION ; PROKARYOTES ; APPARATUS
Funding OrganizationStrategic Pilot Project of Chinese Academy of Sciences [XDA04074002] ; Strategic Pilot Project of Chinese Academy of Sciences [XDA04074002] ; Strategic Pilot Project of Chinese Academy of Sciences [XDA04074002] ; Strategic Pilot Project of Chinese Academy of Sciences [XDA04074002]
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17286
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorHu, CX (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Yu,Li, Xiao-yan,Ge, Hong-Mei,et al. INFLUENCE OF CLINOROTATION ON CELLULAR STRUCTURE, PHOTOSYNTHETIC ACTIVITY, CARBOHYDRATE AND ASTAXANTHIN METABOLISM OF HAEMATOCOCCUS PLUVIALIS[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2012,21(8):2017-2026.
APA Zhang, Yu.,Li, Xiao-yan.,Ge, Hong-Mei.,Wu, Li.,Xia, Ling.,...&Hu, CX .(2012).INFLUENCE OF CLINOROTATION ON CELLULAR STRUCTURE, PHOTOSYNTHETIC ACTIVITY, CARBOHYDRATE AND ASTAXANTHIN METABOLISM OF HAEMATOCOCCUS PLUVIALIS.FRESENIUS ENVIRONMENTAL BULLETIN,21(8),2017-2026.
MLA Zhang, Yu,et al."INFLUENCE OF CLINOROTATION ON CELLULAR STRUCTURE, PHOTOSYNTHETIC ACTIVITY, CARBOHYDRATE AND ASTAXANTHIN METABOLISM OF HAEMATOCOCCUS PLUVIALIS".FRESENIUS ENVIRONMENTAL BULLETIN 21.8(2012):2017-2026.
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