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题名: A flow cytometer based protocol for quantitative analysis of bloom-forming cyanobacteria (Microcystis) in lake sediments
作者: Zhou, Quan1, 2; Chen, Wei1; Zhang, Huiyong3; Peng, Liang1; Liu, Liming1; Han, Zhiguo3; Wan, Neng4; Li, Lin1; Song, Lirong1
通讯作者: Song, LR (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: flow cytometry ; benthic Microcystis ; cell enumeration ; colony size measurement
刊名: JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
发表日期: 2012
DOI: 10.1016/S1001-0742(11)60993-5
卷: 24, 期:9, 页:1709-1716
收录类别: SCI
文章类型: Article
部门归属: [Zhou, Quan; Chen, Wei; Peng, Liang; Liu, Liming; Li, Lin; Song, Lirong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Zhou, Quan] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Zhang, Huiyong; Han, Zhiguo] Zealquest Sci Technol Co Ltd, Zealquest Lab Ecol Res, Shanghai 200333, Peoples R China; [Wan, Neng] Changshu Inst Technol, Changshu 215500, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program (973) of China [2008CB418006]; National Special Program of Water Environment [2009ZX07106-001-002]; National Natural Science Foundation of China [31070355]; National Major Science and Technology Program for Water Pollution Control and Treatment [2009ZX07101-013]
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
摘要: A quantitative protocol for the rapid analysis of Microcystis cells and colonies in lake sediment was developed using a modified flow cytometer, the CytoSense. For cell enumeration, diluted sediment samples containing Microcystis were processed with sonication to disintegrate colonies into single cells. An optimized procedure suggested that 5 mg dw (dry weight)/mL dilution combined with 200 W x 2 min sonication yielded the highest counting efficiency. Under the optimized determination conditions, the quantification limit of this protocol was 3.3x10(4) cells/g dw. For colony analysis, Microcystis were isolated from the sediment by filtration. Colony lengths measured by flow cytometry were similar to those measured by microscopy for the size range of one single cell to almost 400 mu m in length. Moreover, the relationship between colony size and cell number was determined for three Microcystis species, including Microcystis flos-aquae, M. aeruginosa and M. wessenbergii. Regression formulas were used to calculate the cell numbers in different-sized colonies. The developed protocol was applied to field sediment samples from Lake Taihu. The results indicated the potential and applicability of flow cytometry as a tool for the rapid analysis of benthic Microcystis. This study provided a new capability for the high frequency monitoring of benthic overwintering and population dynamics of this bloom-forming cyanobacterium.
英文摘要: A quantitative protocol for the rapid analysis of Microcystis cells and colonies in lake sediment was developed using a modified flow cytometer, the CytoSense. For cell enumeration, diluted sediment samples containing Microcystis were processed with sonication to disintegrate colonies into single cells. An optimized procedure suggested that 5 mg dw (dry weight)/mL dilution combined with 200 W x 2 min sonication yielded the highest counting efficiency. Under the optimized determination conditions, the quantification limit of this protocol was 3.3x10(4) cells/g dw. For colony analysis, Microcystis were isolated from the sediment by filtration. Colony lengths measured by flow cytometry were similar to those measured by microscopy for the size range of one single cell to almost 400 mu m in length. Moreover, the relationship between colony size and cell number was determined for three Microcystis species, including Microcystis flos-aquae, M. aeruginosa and M. wessenbergii. Regression formulas were used to calculate the cell numbers in different-sized colonies. The developed protocol was applied to field sediment samples from Lake Taihu. The results indicated the potential and applicability of flow cytometry as a tool for the rapid analysis of benthic Microcystis. This study provided a new capability for the high frequency monitoring of benthic overwintering and population dynamics of this bloom-forming cyanobacterium.
关键词[WOS]: AERUGINOSA KUTZ ; EUTROPHIC LAKE ; MEILIANG BAY ; PHYTOPLANKTON ; CYANOPHYCEAE ; RECRUITMENT ; SHALLOW ; MARINE ; WATER ; CYTOBUOY
语种: 英语
WOS记录号: WOS:000309371600022
ISSN号: 1001-0742
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17282
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Zealquest Sci Technol Co Ltd, Zealquest Lab Ecol Res, Shanghai 200333, Peoples R China
4.Changshu Inst Technol, Changshu 215500, Peoples R China
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