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题名: Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria
作者: Li, Weizhi1; Gao, Hong1; Yin, Chuntao1; Xu, Xudong1
通讯作者: Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
关键词: SP PCC 6803 ; SYNECHOCYSTIS-SP PCC-6803 ; CHILL-LIGHT TOLERANCE ; SP STRAIN PCC-6803 ; LOW-TEMPERATURE ; PHOTOSYSTEM-II ; SYNECHOCOCCUS SP ; LIPID DIFFUSION ; BTPA PROTEIN ; MEMBRANES
刊名: MICROBIOLOGY-SGM
发表日期: 2012-09-01
DOI: 10.1099/mic.0.060038-0
卷: 158, 期:Part 9, 页:2440-2449
收录类别: SCI
文章类型: Article
部门归属: [Li, Weizhi; Gao, Hong; Yin, Chuntao; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: State Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003]
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
摘要: In cyanobacteria, genes involved in cold acclimation can be upregulated in response to cold stress with or without light. By inactivating 17 such genes in Synechocystis sp. PCC 6803, slr0815 (ccr2) was identified to be a novel gene required for survival at 15 degrees C. It was upregulated by cold stress in the light. Upon exposure to low temperature, a ccr2-null mutant showed greatly reduced photosynthetic and respiratory activities within 12 h relative to the wild-type. At 48 h, the photosystem (PS)II-mediated electron transport in the mutant was reduced to less than one-third of the wild-type level, and the duration of electron transfer from the OB binding site of PSII to PSI was increased to about eight times the wild-type level, whereas the PSI-mediated electron transport remained unchanged. Using an antibody against GFP, a Ccr2-GFP fusion protein was localized to the thylakoid membrane rather than the cytoplasmic and outer membranes. Homologues to Ccr2 can be found in most cyanobacteria, algae and higher plants with sequenced genomes. Ccr2 is probably representative of a group of novel thylakoid proteins involved in acclimation to cold or other stresses.
英文摘要: In cyanobacteria, genes involved in cold acclimation can be upregulated in response to cold stress with or without light. By inactivating 17 such genes in Synechocystis sp. PCC 6803, slr0815 (ccr2) was identified to be a novel gene required for survival at 15 degrees C. It was upregulated by cold stress in the light. Upon exposure to low temperature, a ccr2-null mutant showed greatly reduced photosynthetic and respiratory activities within 12 h relative to the wild-type. At 48 h, the photosystem (PS)II-mediated electron transport in the mutant was reduced to less than one-third of the wild-type level, and the duration of electron transfer from the OB binding site of PSII to PSI was increased to about eight times the wild-type level, whereas the PSI-mediated electron transport remained unchanged. Using an antibody against GFP, a Ccr2-GFP fusion protein was localized to the thylakoid membrane rather than the cytoplasmic and outer membranes. Homologues to Ccr2 can be found in most cyanobacteria, algae and higher plants with sequenced genomes. Ccr2 is probably representative of a group of novel thylakoid proteins involved in acclimation to cold or other stresses.
关键词[WOS]: SP PCC 6803 ; SYNECHOCYSTIS-SP PCC-6803 ; CHILL-LIGHT TOLERANCE ; SP STRAIN PCC-6803 ; LOW-TEMPERATURE ; PHOTOSYSTEM-II ; SYNECHOCOCCUS SP ; LIPID DIFFUSION ; BTPA PROTEIN ; MEMBRANES
语种: 英语
WOS记录号: WOS:000309852800021
ISSN号: 1350-0872
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17276
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Li, Weizhi; Gao, Hong; Yin, Chuntao; Xu, Xudong.Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria,MICROBIOLOGY-SGM,2012,158(Part 9):2440-2449
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