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Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria
Li, Weizhi; Gao, Hong; Yin, Chuntao; Xu, Xudong; Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
2012-09-01
Source PublicationMICROBIOLOGY-SGM
ISSN1350-0872
Volume158Issue:Part 9Pages:2440-2449
AbstractIn cyanobacteria, genes involved in cold acclimation can be upregulated in response to cold stress with or without light. By inactivating 17 such genes in Synechocystis sp. PCC 6803, slr0815 (ccr2) was identified to be a novel gene required for survival at 15 degrees C. It was upregulated by cold stress in the light. Upon exposure to low temperature, a ccr2-null mutant showed greatly reduced photosynthetic and respiratory activities within 12 h relative to the wild-type. At 48 h, the photosystem (PS)II-mediated electron transport in the mutant was reduced to less than one-third of the wild-type level, and the duration of electron transfer from the OB binding site of PSII to PSI was increased to about eight times the wild-type level, whereas the PSI-mediated electron transport remained unchanged. Using an antibody against GFP, a Ccr2-GFP fusion protein was localized to the thylakoid membrane rather than the cytoplasmic and outer membranes. Homologues to Ccr2 can be found in most cyanobacteria, algae and higher plants with sequenced genomes. Ccr2 is probably representative of a group of novel thylakoid proteins involved in acclimation to cold or other stresses.; In cyanobacteria, genes involved in cold acclimation can be upregulated in response to cold stress with or without light. By inactivating 17 such genes in Synechocystis sp. PCC 6803, slr0815 (ccr2) was identified to be a novel gene required for survival at 15 degrees C. It was upregulated by cold stress in the light. Upon exposure to low temperature, a ccr2-null mutant showed greatly reduced photosynthetic and respiratory activities within 12 h relative to the wild-type. At 48 h, the photosystem (PS)II-mediated electron transport in the mutant was reduced to less than one-third of the wild-type level, and the duration of electron transfer from the OB binding site of PSII to PSI was increased to about eight times the wild-type level, whereas the PSI-mediated electron transport remained unchanged. Using an antibody against GFP, a Ccr2-GFP fusion protein was localized to the thylakoid membrane rather than the cytoplasmic and outer membranes. Homologues to Ccr2 can be found in most cyanobacteria, algae and higher plants with sequenced genomes. Ccr2 is probably representative of a group of novel thylakoid proteins involved in acclimation to cold or other stresses.
SubtypeArticle
KeywordSp Pcc 6803 Synechocystis-sp Pcc-6803 Chill-light Tolerance Sp Strain Pcc-6803 Low-temperature Photosystem-ii Synechococcus Sp Lipid Diffusion Btpa Protein Membranes
Department[Li, Weizhi; Gao, Hong; Yin, Chuntao; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
DOI10.1099/mic.0.060038-0
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationState Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003] ; State Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003] ; State Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003] ; State Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003]
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000309852800021
WOS KeywordSP PCC 6803 ; SYNECHOCYSTIS-SP PCC-6803 ; CHILL-LIGHT TOLERANCE ; SP STRAIN PCC-6803 ; LOW-TEMPERATURE ; PHOTOSYSTEM-II ; SYNECHOCOCCUS SP ; LIPID DIFFUSION ; BTPA PROTEIN ; MEMBRANES
Funding OrganizationState Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003] ; State Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003] ; State Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003] ; State Key Basic Research Development Program of China [2008CB418001]; National Natural Science Foundation of China [30825003]
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17276
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Li, Weizhi,Gao, Hong,Yin, Chuntao,et al. Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria[J]. MICROBIOLOGY-SGM,2012,158(Part 9):2440-2449.
APA Li, Weizhi,Gao, Hong,Yin, Chuntao,Xu, Xudong,&Xu, XD .(2012).Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria.MICROBIOLOGY-SGM,158(Part 9),2440-2449.
MLA Li, Weizhi,et al."Identification of a novel thylakoid protein gene involved in cold acclimation in cyanobacteria".MICROBIOLOGY-SGM 158.Part 9(2012):2440-2449.
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