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Conformational changes in photosynthetic pigment proteins on thylakoid membranes can lead to fast non-photochemical quenching in cyanobacteria
Wang ZhiCong1,2; Dong Jing1,2; Li DunHai1; Li, DH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2012-08-01
Source PublicationSCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
Volume55Issue:8Pages:726-734
AbstractA high non-photochemical quenching (NPQ) appeared below the phase transition temperature when Microcystis aeruginosa PCC7806 cells were exposed to saturated light for a short time. This suggested that a component of NPQ, independent from state transition or photo-inhibition, had been generated in the PSII complex; this was a fast component responding to high intensity light. Glutaraldehyde (GA), commonly used to stabilize membrane protein conformations, resulted in more energy transfer to PSII reaction centers, affecting the energy absorption and dissipation process rather than the transfer process of phycobilisome (PBS). In comparison experiments with and without GA, the rapid light curves (RLCs) and fluorescence induction dynamics of the fast phase showed that excess excitation energy was dissipated by conformational change in the photosynthetic pigment proteins on the thylakoid membrane (PPPTM). Based on deconvolution of NPQ relaxation kinetics, we concluded that the fast quenching component (NPQf) was closely related to PPPTM conformational change, as it accounted for as much as 39.42% of the total NPQ. We hypothesize therefore, that NPQf induced by PPPTM conformation is an important adaptation mechanism for Microcystis blooms under high-intensity light during summer and autumn.; A high non-photochemical quenching (NPQ) appeared below the phase transition temperature when Microcystis aeruginosa PCC7806 cells were exposed to saturated light for a short time. This suggested that a component of NPQ, independent from state transition or photo-inhibition, had been generated in the PSII complex; this was a fast component responding to high intensity light. Glutaraldehyde (GA), commonly used to stabilize membrane protein conformations, resulted in more energy transfer to PSII reaction centers, affecting the energy absorption and dissipation process rather than the transfer process of phycobilisome (PBS). In comparison experiments with and without GA, the rapid light curves (RLCs) and fluorescence induction dynamics of the fast phase showed that excess excitation energy was dissipated by conformational change in the photosynthetic pigment proteins on the thylakoid membrane (PPPTM). Based on deconvolution of NPQ relaxation kinetics, we concluded that the fast quenching component (NPQf) was closely related to PPPTM conformational change, as it accounted for as much as 39.42% of the total NPQ. We hypothesize therefore, that NPQf induced by PPPTM conformation is an important adaptation mechanism for Microcystis blooms under high-intensity light during summer and autumn.
SubtypeArticle
KeywordChlorophyll Fluorescence Photoprotection Microcystis Aeruginosa Non-photochemical Quenching Thylakoid Membrane Proteins
Department[Wang ZhiCong; Dong Jing; Li DunHai] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Wang ZhiCong; Dong Jing] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
DOI10.1007/s11427-012-4360-5
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001] ; National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001] ; National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001] ; National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001]
Indexed BySCI
Language英语
WOS Research AreaLife Sciences & Biomedicine - Other Topics
WOS SubjectBiology
WOS IDWOS:000308127100010
WOS KeywordPHOTOSYSTEM-II FLUORESCENCE ; ORANGE CAROTENOID PROTEIN ; SYNECHOCYSTIS PCC 6803 ; CHLOROPHYLL FLUORESCENCE ; ENERGY-DISSIPATION ; PORPHYRIDIUM-CRUENTUM ; ELECTRON-TRANSPORT ; MICROCYSTIS BLOOM ; RED ALGA ; MECHANISM
Funding OrganizationNational Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001] ; National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001] ; National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001] ; National Basic Research Program of China [2008CB418002]; National Major Programs of Water Body Pollution Control and Remediation [2009ZX07104-005, 2009ZX07106-001]
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Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17254
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLi, DH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Wang ZhiCong,Dong Jing,Li DunHai,et al. Conformational changes in photosynthetic pigment proteins on thylakoid membranes can lead to fast non-photochemical quenching in cyanobacteria[J]. SCIENCE CHINA-LIFE SCIENCES,2012,55(8):726-734.
APA Wang ZhiCong,Dong Jing,Li DunHai,&Li, DH .(2012).Conformational changes in photosynthetic pigment proteins on thylakoid membranes can lead to fast non-photochemical quenching in cyanobacteria.SCIENCE CHINA-LIFE SCIENCES,55(8),726-734.
MLA Wang ZhiCong,et al."Conformational changes in photosynthetic pigment proteins on thylakoid membranes can lead to fast non-photochemical quenching in cyanobacteria".SCIENCE CHINA-LIFE SCIENCES 55.8(2012):726-734.
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