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Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae
Chen, Lianguo1,2; Huang, Changjiang3; Hu, Chenyan1; Yu, Ke1; Yang, Lihua1; Zhou, Bingsheng1; Zhou, BS (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China.
2012-10-01
Source PublicationENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
ISSN0730-7268
Volume31Issue:10Pages:2338-2344
AbstractThe aim of the present study was to investigate the acute developmental neurotoxicity of polybrominated diphenyl ethers (PBDEs) in zebrafish larvae. From 2 to 120?h postfertilization zebrafish embryos were exposed to DE-71 (0, 31.0, 68.7, and 227.6?mu g/L). The authors studied the locomotor behavior of larvae, involvement of the cholinergic system, and selected gene and protein expressions in the central nervous system. The results showed that low DE-71 concentration caused hyperactivity, whereas higher concentrations decreased activity during the dark period. During the light period, larval activity was significantly reduced in a concentration-dependent manner. In the cholinergic system, acetylcholinesterase activity significantly increased (10.7 and 12.4%) in the 68.7 and 227.6?mu g/L exposure groups, respectively, and acetylcholine concentration accordingly decreased (60.5%) in the 227.6?mu g/L exposure group. The mRNA expressions of genes encoding myelin basic protein, neuron microtubule protein (a1-tubulin), and sonic hedgehog a were significantly downregulated. Western blotting assay demonstrated that the protein concentration of a1-tubulin was also decreased. Overall, the present study demonstrated that acute exposure to PBDEs can disrupt the neurobehavior of zebrafish larvae and affect cholinergic neurotransmission and neuron development. Environ. Toxicol. Chem. 2012; 31: 23382344. (c) 2012 SETAC; The aim of the present study was to investigate the acute developmental neurotoxicity of polybrominated diphenyl ethers (PBDEs) in zebrafish larvae. From 2 to 120?h postfertilization zebrafish embryos were exposed to DE-71 (0, 31.0, 68.7, and 227.6?mu g/L). The authors studied the locomotor behavior of larvae, involvement of the cholinergic system, and selected gene and protein expressions in the central nervous system. The results showed that low DE-71 concentration caused hyperactivity, whereas higher concentrations decreased activity during the dark period. During the light period, larval activity was significantly reduced in a concentration-dependent manner. In the cholinergic system, acetylcholinesterase activity significantly increased (10.7 and 12.4%) in the 68.7 and 227.6?mu g/L exposure groups, respectively, and acetylcholine concentration accordingly decreased (60.5%) in the 227.6?mu g/L exposure group. The mRNA expressions of genes encoding myelin basic protein, neuron microtubule protein (a1-tubulin), and sonic hedgehog a were significantly downregulated. Western blotting assay demonstrated that the protein concentration of a1-tubulin was also decreased. Overall, the present study demonstrated that acute exposure to PBDEs can disrupt the neurobehavior of zebrafish larvae and affect cholinergic neurotransmission and neuron development. Environ. Toxicol. Chem. 2012; 31: 23382344. (c) 2012 SETAC
SubtypeArticle
KeywordPolybrominated Diphenyl Ethers Developmental Neurotoxicity Cholinergic System Locomotor Behavior Zebrafish Embryos
Department[Chen, Lianguo; Hu, Chenyan; Yu, Ke; Yang, Lihua; Zhou, Bingsheng] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China; [Chen, Lianguo] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Huang, Changjiang] Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou, Peoples R China
DOI10.1002/etc.1958
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology ; Toxicology
WOS SubjectEnvironmental Sciences ; Toxicology
WOS IDWOS:000308928700019
WOS KeywordPOLYBROMINATED DIPHENYL ETHERS ; BROMINATED FLAME-RETARDANT ; NEONATAL EXPOSURE ; NEURONAL DEVELOPMENT ; EMBRYONIC EXPOSURE ; MATERNAL TRANSFER ; CEREBRAL-CORTEX ; EXPRESSION ; GROWTH ; SYNAPTOGENESIS
Funding OrganizationNational Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13] ; National Natural Science Foundation of China [20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology [2011FBZ13]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17250
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou, Peoples R China
Recommended Citation
GB/T 7714
Chen, Lianguo,Huang, Changjiang,Hu, Chenyan,et al. Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae[J]. ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,2012,31(10):2338-2344.
APA Chen, Lianguo.,Huang, Changjiang.,Hu, Chenyan.,Yu, Ke.,Yang, Lihua.,...&Zhou, BS .(2012).Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae.ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY,31(10),2338-2344.
MLA Chen, Lianguo,et al."Acute exposure to DE-71: Effects on locomotor behavior and developmental neurotoxicity in zebrafish larvae".ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY 31.10(2012):2338-2344.
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