IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Medaka piwi is Essential for Primordial Germ Cell Migration
Li, M.1,2; Hong, N.1; Gui, J.2; Hong, Y.1; Hong, Y (reprint author), Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr 4, Singapore 117543, Singapore.
2012-09-01
Source PublicationCURRENT MOLECULAR MEDICINE
ISSN1566-5240
Volume12Issue:8Pages:1040-1049
AbstractPiwi controls the number of primordial germ cells (PGCs) via protecting maternal mRNA from decay and adult germ stem cell division in Drosophila. In mouse and zebrafish, piwi controls maintenance and differentiation of adult germ stem cell during gametogenesis. Whether piwi plays a role in PGC development of vertebrates remains unsolved. We addressed this issue by using medaka (Oryzias latipes) as a vertebrate model. Molecular cloning, sequence comparison and analyses of genomic organization and chromosome synteny led to the identification in this fish of a single piwi gene, called Opiwi. By RT-PCR analyses and in situ hybridization, the Opiwi transcript is maternally supplied and becomes restricted to PGCs and the central nervous system (CNS). Opiwi knockdown did not prevent PGC formation even in the absence of any somatic structures but did significantly reduce the number of PGCs in vivo and in vitro and affect the distribution of PGCs in developing embryos. Surprisingly, depletion of zygotic Opiwi severely and specifically affected PGC migration. We conclude that Opiwi is required not only for determining the PGC number but also for controlling PGC migration. Our results demonstrate that piwi plays a generally conserved role in germ cell development from Drosophila to vertebrate and a specific role in PGC migration.; Piwi controls the number of primordial germ cells (PGCs) via protecting maternal mRNA from decay and adult germ stem cell division in Drosophila. In mouse and zebrafish, piwi controls maintenance and differentiation of adult germ stem cell during gametogenesis. Whether piwi plays a role in PGC development of vertebrates remains unsolved. We addressed this issue by using medaka (Oryzias latipes) as a vertebrate model. Molecular cloning, sequence comparison and analyses of genomic organization and chromosome synteny led to the identification in this fish of a single piwi gene, called Opiwi. By RT-PCR analyses and in situ hybridization, the Opiwi transcript is maternally supplied and becomes restricted to PGCs and the central nervous system (CNS). Opiwi knockdown did not prevent PGC formation even in the absence of any somatic structures but did significantly reduce the number of PGCs in vivo and in vitro and affect the distribution of PGCs in developing embryos. Surprisingly, depletion of zygotic Opiwi severely and specifically affected PGC migration. We conclude that Opiwi is required not only for determining the PGC number but also for controlling PGC migration. Our results demonstrate that piwi plays a generally conserved role in germ cell development from Drosophila to vertebrate and a specific role in PGC migration.
SubtypeReview
KeywordCell Migration Embryo Germ Cell Motility Piwi
Department[Li, M.; Hong, N.; Hong, Y.] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore; [Li, M.; Gui, J.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationBiomedical Research Council of Singapore [R-08-1-21-19-585]; National Research Foundation of Singapore [R-154-000-529-281]; National Key Basic Research Program of China [2010CB126301] ; Biomedical Research Council of Singapore [R-08-1-21-19-585]; National Research Foundation of Singapore [R-154-000-529-281]; National Key Basic Research Program of China [2010CB126301] ; Biomedical Research Council of Singapore [R-08-1-21-19-585]; National Research Foundation of Singapore [R-154-000-529-281]; National Key Basic Research Program of China [2010CB126301] ; Biomedical Research Council of Singapore [R-08-1-21-19-585]; National Research Foundation of Singapore [R-154-000-529-281]; National Key Basic Research Program of China [2010CB126301]
Indexed BySCI
Language英语
WOS Research AreaResearch & Experimental Medicine
WOS SubjectMedicine, Research & Experimental
WOS IDWOS:000307950000013
WOS KeywordRNA-BINDING PROTEIN ; ORYZIAS-LATIPES ; STEM-CELLS ; SELF-RENEWAL ; GENE FAMILY ; FISH ; MILI ; SPERMATOGENESIS ; DIFFERENTIATION ; MODEL
Funding OrganizationBiomedical Research Council of Singapore [R-08-1-21-19-585]; National Research Foundation of Singapore [R-154-000-529-281]; National Key Basic Research Program of China [2010CB126301] ; Biomedical Research Council of Singapore [R-08-1-21-19-585]; National Research Foundation of Singapore [R-154-000-529-281]; National Key Basic Research Program of China [2010CB126301] ; Biomedical Research Council of Singapore [R-08-1-21-19-585]; National Research Foundation of Singapore [R-154-000-529-281]; National Key Basic Research Program of China [2010CB126301] ; Biomedical Research Council of Singapore [R-08-1-21-19-585]; National Research Foundation of Singapore [R-154-000-529-281]; National Key Basic Research Program of China [2010CB126301]
Citation statistics
Cited Times:30[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17238
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorHong, Y (reprint author), Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr 4, Singapore 117543, Singapore.
Affiliation1.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Li, M.,Hong, N.,Gui, J.,et al. Medaka piwi is Essential for Primordial Germ Cell Migration[J]. CURRENT MOLECULAR MEDICINE,2012,12(8):1040-1049.
APA Li, M.,Hong, N.,Gui, J.,Hong, Y.,&Hong, Y .(2012).Medaka piwi is Essential for Primordial Germ Cell Migration.CURRENT MOLECULAR MEDICINE,12(8),1040-1049.
MLA Li, M.,et al."Medaka piwi is Essential for Primordial Germ Cell Migration".CURRENT MOLECULAR MEDICINE 12.8(2012):1040-1049.
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