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题名: Medaka piwi is Essential for Primordial Germ Cell Migration
作者: Li, M.1, 2; Hong, N.1; Gui, J.2; Hong, Y.1
通讯作者: Hong, Y (reprint author), Natl Univ Singapore, Dept Biol Sci, 14 Sci Dr 4, Singapore 117543, Singapore.
关键词: Cell migration ; embryo ; germ cell ; motility ; piwi
刊名: CURRENT MOLECULAR MEDICINE
发表日期: 2012-09-01
卷: 12, 期:8, 页:1040-1049
收录类别: SCI
文章类型: Review
部门归属: [Li, M.; Hong, N.; Hong, Y.] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore; [Li, M.; Gui, J.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Biomedical Research Council of Singapore [R-08-1-21-19-585]; National Research Foundation of Singapore [R-154-000-529-281]; National Key Basic Research Program of China [2010CB126301]
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
摘要: Piwi controls the number of primordial germ cells (PGCs) via protecting maternal mRNA from decay and adult germ stem cell division in Drosophila. In mouse and zebrafish, piwi controls maintenance and differentiation of adult germ stem cell during gametogenesis. Whether piwi plays a role in PGC development of vertebrates remains unsolved. We addressed this issue by using medaka (Oryzias latipes) as a vertebrate model. Molecular cloning, sequence comparison and analyses of genomic organization and chromosome synteny led to the identification in this fish of a single piwi gene, called Opiwi. By RT-PCR analyses and in situ hybridization, the Opiwi transcript is maternally supplied and becomes restricted to PGCs and the central nervous system (CNS). Opiwi knockdown did not prevent PGC formation even in the absence of any somatic structures but did significantly reduce the number of PGCs in vivo and in vitro and affect the distribution of PGCs in developing embryos. Surprisingly, depletion of zygotic Opiwi severely and specifically affected PGC migration. We conclude that Opiwi is required not only for determining the PGC number but also for controlling PGC migration. Our results demonstrate that piwi plays a generally conserved role in germ cell development from Drosophila to vertebrate and a specific role in PGC migration.
英文摘要: Piwi controls the number of primordial germ cells (PGCs) via protecting maternal mRNA from decay and adult germ stem cell division in Drosophila. In mouse and zebrafish, piwi controls maintenance and differentiation of adult germ stem cell during gametogenesis. Whether piwi plays a role in PGC development of vertebrates remains unsolved. We addressed this issue by using medaka (Oryzias latipes) as a vertebrate model. Molecular cloning, sequence comparison and analyses of genomic organization and chromosome synteny led to the identification in this fish of a single piwi gene, called Opiwi. By RT-PCR analyses and in situ hybridization, the Opiwi transcript is maternally supplied and becomes restricted to PGCs and the central nervous system (CNS). Opiwi knockdown did not prevent PGC formation even in the absence of any somatic structures but did significantly reduce the number of PGCs in vivo and in vitro and affect the distribution of PGCs in developing embryos. Surprisingly, depletion of zygotic Opiwi severely and specifically affected PGC migration. We conclude that Opiwi is required not only for determining the PGC number but also for controlling PGC migration. Our results demonstrate that piwi plays a generally conserved role in germ cell development from Drosophila to vertebrate and a specific role in PGC migration.
关键词[WOS]: RNA-BINDING PROTEIN ; ORYZIAS-LATIPES ; STEM-CELLS ; SELF-RENEWAL ; GENE FAMILY ; FISH ; MILI ; SPERMATOGENESIS ; DIFFERENTIATION ; MODEL
语种: 英语
WOS记录号: WOS:000307950000013
ISSN号: 1566-5240
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17238
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Li, M.; Hong, N.; Gui, J.; Hong, Y..Medaka piwi is Essential for Primordial Germ Cell Migration,CURRENT MOLECULAR MEDICINE,2012,12(8):1040-1049
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