IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis)
Liu, Lusha1,2; Yu, Xiaomu1; Tong, Jingou1; Tong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2012-09-01
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume39Issue:9Pages:9211-9221
AbstractMyostatin (MSTN) is a member of the transforming growth factor-beta superfamily and functions as a negative regulator of skeletal muscle development and growth. In this study, the bighead carp MSTN gene (AnMSTN for short) was cloned and characterized. The 3,769 bp genomic sequence of AnMSTN consisted of three exons and two introns, and the full length cDNA (2,141 bp) of the gene had an open reading frame encoding a polypeptide of 375 amino acids. The deduced amino acid sequence of AnMSTN showed 67.1-98.7 % homology with MSTNs of avian, mammalian and teleostean species. Sequence comparison and phylogenetic analysis confirmed the MSTNs were conserved throughout the vertebrates and AnMSTN belonged to MSNT-1 isoform. AnMSTN was expressed in various tissues with the highest expression in muscle. Two single nucleotide polymorphisms, g.1668T > C in intron 2 and g.2770C > A in 3' UTR, were identified in AnMSTN by sequencing PCR fragments, and genotyped by SSCP. Association analysis showed that g.2770C > A genotypes were significantly associated with total length, body length and body weight (P < 0.01). These results suggest that AnMSTN involves in the regulation of growth, and this polymorphism would be informative for further studies on selective breeding in bighead carp.; Myostatin (MSTN) is a member of the transforming growth factor-beta superfamily and functions as a negative regulator of skeletal muscle development and growth. In this study, the bighead carp MSTN gene (AnMSTN for short) was cloned and characterized. The 3,769 bp genomic sequence of AnMSTN consisted of three exons and two introns, and the full length cDNA (2,141 bp) of the gene had an open reading frame encoding a polypeptide of 375 amino acids. The deduced amino acid sequence of AnMSTN showed 67.1-98.7 % homology with MSTNs of avian, mammalian and teleostean species. Sequence comparison and phylogenetic analysis confirmed the MSTNs were conserved throughout the vertebrates and AnMSTN belonged to MSNT-1 isoform. AnMSTN was expressed in various tissues with the highest expression in muscle. Two single nucleotide polymorphisms, g.1668T > C in intron 2 and g.2770C > A in 3' UTR, were identified in AnMSTN by sequencing PCR fragments, and genotyped by SSCP. Association analysis showed that g.2770C > A genotypes were significantly associated with total length, body length and body weight (P < 0.01). These results suggest that AnMSTN involves in the regulation of growth, and this polymorphism would be informative for further studies on selective breeding in bighead carp.
SubtypeArticle
KeywordMstn Characterization Snp Polymorphism Genetic Association Bighead Carp (Aristichthys Nobilis)
Department[Liu, Lusha; Yu, Xiaomu; Tong, Jingou] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Liu, Lusha] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
DOI10.1007/s11033-012-1794-6
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationMinistry of Agriculture of China [200903045, 2011-G12]; CAS ; Ministry of Agriculture of China [200903045, 2011-G12]; CAS ; Ministry of Agriculture of China [200903045, 2011-G12]; CAS ; Ministry of Agriculture of China [200903045, 2011-G12]; CAS
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000306799700075
WOS KeywordVERTEBRATE GENOME EVOLUTION ; BETA SUPERFAMILY MEMBER ; DIFFERENTIAL EXPRESSION ; TISSUE EXPRESSION ; ZEBRAFISH ; MUSCLE ; POLYMORPHISMS ; CLONING ; CATTLE ; SHEEP
Funding OrganizationMinistry of Agriculture of China [200903045, 2011-G12]; CAS ; Ministry of Agriculture of China [200903045, 2011-G12]; CAS ; Ministry of Agriculture of China [200903045, 2011-G12]; CAS ; Ministry of Agriculture of China [200903045, 2011-G12]; CAS
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Cited Times:25[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17236
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorTong, JG (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Liu, Lusha,Yu, Xiaomu,Tong, Jingou,et al. Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis)[J]. MOLECULAR BIOLOGY REPORTS,2012,39(9):9211-9221.
APA Liu, Lusha,Yu, Xiaomu,Tong, Jingou,&Tong, JG .(2012).Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis).MOLECULAR BIOLOGY REPORTS,39(9),9211-9221.
MLA Liu, Lusha,et al."Molecular characterization of myostatin (MSTN) gene and association analysis with growth traits in the bighead carp (Aristichthys nobilis)".MOLECULAR BIOLOGY REPORTS 39.9(2012):9211-9221.
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