IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Sequencing and analysis of the complete genome of Rana grylio virus (RGV)
Lei, Xiao-Ying; Ou, Tong; Zhu, Ruo-Lin; Zhang, Qi-Ya; Zhang, QY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2012-08-01
Source PublicationARCHIVES OF VIROLOGY
ISSN0304-8608
Volume157Issue:8Pages:1559-1564
AbstractInfection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses.; Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses.
SubtypeArticle
KeywordLymphocystis Disease Virus Ranavirus Family Iridoviridae Dna-sequence Fish Cells Micrornas Gene Identification Expression Apoptosis Repeats
Department[Lei, Xiao-Ying; Ou, Tong; Zhu, Ruo-Lin; Zhang, Qi-Ya] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1007/s00705-012-1316-9
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01]
Indexed BySCI
Language英语
WOS Research AreaVirology
WOS SubjectVirology
WOS IDWOS:000307253800014
WOS KeywordLYMPHOCYSTIS DISEASE VIRUS ; RANAVIRUS FAMILY IRIDOVIRIDAE ; DNA-SEQUENCE ; FISH CELLS ; MICRORNAS ; GENE ; IDENTIFICATION ; EXPRESSION ; APOPTOSIS ; REPEATS
Funding OrganizationNational Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17234
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorZhang, QY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Lei, Xiao-Ying,Ou, Tong,Zhu, Ruo-Lin,et al. Sequencing and analysis of the complete genome of Rana grylio virus (RGV)[J]. ARCHIVES OF VIROLOGY,2012,157(8):1559-1564.
APA Lei, Xiao-Ying,Ou, Tong,Zhu, Ruo-Lin,Zhang, Qi-Ya,&Zhang, QY .(2012).Sequencing and analysis of the complete genome of Rana grylio virus (RGV).ARCHIVES OF VIROLOGY,157(8),1559-1564.
MLA Lei, Xiao-Ying,et al."Sequencing and analysis of the complete genome of Rana grylio virus (RGV)".ARCHIVES OF VIROLOGY 157.8(2012):1559-1564.
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