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题名: Sequencing and analysis of the complete genome of Rana grylio virus (RGV)
作者: Lei, Xiao-Ying1; Ou, Tong1; Zhu, Ruo-Lin1; Zhang, Qi-Ya1
通讯作者: Zhang, QY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
关键词: LYMPHOCYSTIS DISEASE VIRUS ; RANAVIRUS FAMILY IRIDOVIRIDAE ; DNA-SEQUENCE ; FISH CELLS ; MICRORNAS ; GENE ; IDENTIFICATION ; EXPRESSION ; APOPTOSIS ; REPEATS
刊名: ARCHIVES OF VIROLOGY
发表日期: 2012-08-01
DOI: 10.1007/s00705-012-1316-9
卷: 157, 期:8, 页:1559-1564
收录类别: SCI
文章类型: Article
部门归属: [Lei, Xiao-Ying; Ou, Tong; Zhu, Ruo-Lin; Zhang, Qi-Ya] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Major Basic Research Program [2010CB126303, 2009CB118704]; Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Natural Science Foundation of China [31072239]; FEBL research grant [Y15B121F01]
类目[WOS]: Virology
研究领域[WOS]: Virology
摘要: Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses.
英文摘要: Infection with Rana grylio virus (RGV), an iridovirus isolated in China in 1995, resulted in a high mortality rate in frogs. The complete genome sequence of RGV was determined and analyzed. The genomic DNA was 105,791 bp long, with 106 open reading frames (ORFs). Dot plot analysis showed that the gene order of RGV shared colinearity with three completely sequenced ranaviruses. A phylogenetic tree was constructed based on concatenated sequences of iridovirus 26 core-gene-encoded proteins, and the result showed high bootstrap support for RGV being a member of the genus Ranavirus and that iridoviruses of other genera also clustered closely. A microRNA (miRNA) prediction revealed that RGV could encode 18 mature miRNAs, many of which were located near genes associated with virus replication. Thirty-three repeated sequences were found in the RGV genome. These results provide insight into the genetic nature of RGV and are useful for laboratory diagnosis for vertebrate iridoviruses.
关键词[WOS]: LYMPHOCYSTIS DISEASE VIRUS ; RANAVIRUS FAMILY IRIDOVIRIDAE ; DNA-SEQUENCE ; FISH CELLS ; MICRORNAS ; GENE ; IDENTIFICATION ; EXPRESSION ; APOPTOSIS ; REPEATS
语种: 英语
WOS记录号: WOS:000307253800014
ISSN号: 0304-8608
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17234
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China

Recommended Citation:
Lei, Xiao-Ying; Ou, Tong; Zhu, Ruo-Lin; Zhang, Qi-Ya.Sequencing and analysis of the complete genome of Rana grylio virus (RGV),ARCHIVES OF VIROLOGY,2012,157(8):1559-1564
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文件名: Sequencing and analysis of the complete genome of Rana grylio virus (RGV).pdf
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