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题名: Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns
作者: Lei, Xiao-Ying1; Ou, Tong1; Zhang, Qi-Ya1
通讯作者: Lei, XY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
关键词: MAJOR CAPSID PROTEIN ; FISH CELL-LINE ; FAMILY IRIDOVIRIDAE ; SEQUENCE-ANALYSIS ; GENE ; EXPRESSION ; DNA ; MORPHOGENESIS ; REPLICATION ; TRANSPORT
刊名: PLOS ONE
发表日期: 2012-08-13
DOI: 10.1371/journal.pone.0043033
卷: 7, 期:8, 页:e43033
收录类别: SCI
文章类型: Article
部门归属: [Lei, Xiao-Ying; Ou, Tong; Zhang, Qi-Ya] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
WOS标题词: Science & Technology
资助者: National Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Background: The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50L have not been studied yet. Methodology/Principal Findings: We cloned and characterized RGV50L, and revealed 50L functions in virus assembly and gene regulation. 50L encoded a 499-amino acid structural protein of about 85 kDa in molecular weight and contained a nuclear localization signal (NLS) and a helix-extension-helix motif. Drug inhibition assay demonstrated that 50L was an immediate-early (IE) gene. Immuno-fluorescence assay revealed that 50L appeared early and persisted in RGV-infected cells following two distribution patterns. One pattern was that 50L exhibited a cytoplasm-nucleus-viromatrix distribution pattern, and mutagenesis of the NLS motif revealed that localization of 50L in the nucleus was NLS-dependent; the other was that 50L co-localized with viral matrix which plays important roles in virus assembly and the life circle of viruses. Conclusion/Significance: RGV 50L is a novel iridovirus IE gene encoded structural protein which plays important roles in virus assembly.
英文摘要: Background: The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50L have not been studied yet.
关键词[WOS]: MAJOR CAPSID PROTEIN ; FISH CELL-LINE ; FAMILY IRIDOVIRIDAE ; SEQUENCE-ANALYSIS ; GENE ; EXPRESSION ; DNA ; MORPHOGENESIS ; REPLICATION ; TRANSPORT
语种: 英语
WOS记录号: WOS:000307500100055
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17232
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China

Recommended Citation:
Lei, Xiao-Ying; Ou, Tong; Zhang, Qi-Ya.Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns,PLOS ONE,2012,7(8):e43033
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文件名: Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns.pdf
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