IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns
Lei, Xiao-Ying; Ou, Tong; Zhang, Qi-Ya; Lei, XY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
2012-08-13
Source PublicationPLOS ONE
ISSN1932-6203
Volume7Issue:8Pages:e43033
AbstractBackground: The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50L have not been studied yet. Methodology/Principal Findings: We cloned and characterized RGV50L, and revealed 50L functions in virus assembly and gene regulation. 50L encoded a 499-amino acid structural protein of about 85 kDa in molecular weight and contained a nuclear localization signal (NLS) and a helix-extension-helix motif. Drug inhibition assay demonstrated that 50L was an immediate-early (IE) gene. Immuno-fluorescence assay revealed that 50L appeared early and persisted in RGV-infected cells following two distribution patterns. One pattern was that 50L exhibited a cytoplasm-nucleus-viromatrix distribution pattern, and mutagenesis of the NLS motif revealed that localization of 50L in the nucleus was NLS-dependent; the other was that 50L co-localized with viral matrix which plays important roles in virus assembly and the life circle of viruses. Conclusion/Significance: RGV 50L is a novel iridovirus IE gene encoded structural protein which plays important roles in virus assembly.; Background: The complete genome of Rana grylio virus (RGV) was sequenced and analyzed recently, which revealed that RGV 50L had homologues in many iridoviruses with different identities; however, the characteristics and functions of 50L have not been studied yet.
SubtypeArticle
KeywordMajor Capsid Protein Fish Cell-line Family Iridoviridae Sequence-analysis Gene Expression Dna Morphogenesis Replication Transport
Department[Lei, Xiao-Ying; Ou, Tong; Zhang, Qi-Ya] Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
DOI10.1371/journal.pone.0043033
WOS HeadingsScience & Technology
Funding OrganizationNational Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000307500100055
WOS KeywordMAJOR CAPSID PROTEIN ; FISH CELL-LINE ; FAMILY IRIDOVIRIDAE ; SEQUENCE-ANALYSIS ; GENE ; EXPRESSION ; DNA ; MORPHOGENESIS ; REPLICATION ; TRANSPORT
Funding OrganizationNational Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01] ; National Major Basic Research Program [2010CB126303, 2009CB118704]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; National Key Technologies R & D Program of China [2012BAD25B00]; National Natural Science Foundation of China [31072239]; FEBL [Y15B121F01]
Citation statistics
Cited Times:14[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17232
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLei, XY (reprint author), Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China.
AffiliationChinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Lei, Xiao-Ying,Ou, Tong,Zhang, Qi-Ya,et al. Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns[J]. PLOS ONE,2012,7(8):e43033.
APA Lei, Xiao-Ying,Ou, Tong,Zhang, Qi-Ya,&Lei, XY .(2012).Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns.PLOS ONE,7(8),e43033.
MLA Lei, Xiao-Ying,et al."Rana grylio Virus (RGV) 50L Is Associated with Viral Matrix and Exhibited Two Distribution Patterns".PLOS ONE 7.8(2012):e43033.
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