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题名: Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus
作者: Huang, Rong; Dong, Feng2; Jang, Songhun2; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping1
通讯作者: Wang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Fish Biol & Fishery Biotechnol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
关键词: Grass carp ; BAC library ; tlr4 gene cluster ; Grass carp reovirus
刊名: DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
发表日期: 2012-10-01
DOI: 10.1016/j.dci.2012.06.002
卷: 38, 期:2, 页:383-388
收录类别: SCI
文章类型: Article
部门归属: [Wang, Yaping] Chinese Acad Sci, Inst Hydrobiol, Ctr Fish Biol & Fishery Biotechnol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; [Dong, Feng; Jang, Songhun] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3]
类目[WOS]: Immunology ; Zoology
研究领域[WOS]: Immunology ; Zoology
摘要: The mammalian response to lipopolysaccharide (LPS) is mainly mediated by Toll-Like Receptor 4 (TLR4). Fish and mammalian TLR4 vary; fish TLR4 ligands are unknown. Isolation of fish tlr4 genes is difficult due to their complex genomic structure. Three bacterial artificial chromosome (BAC) clones containing grass carp tlr4 were obtained. Four tlr4 genes, with a varied genomic structure and different protein domains were subsequently isolated by constructing a subcloned library and rapid amplification of cDNA ends (RACE). The four tlr4 genes were expressed during development from 12 h post-fertilization, in all healthy adult fish tissues tested, and significantly increased in grass carp reovirus (GCRV)-infected liver and muscle, suggesting the tlr4 genes play a role in GCRV infection. This study effectively separated each gene in the tlr4 gene cluster, implies that grass carp TLR4 proteins have different ligand recognition specificities to mammalian TLRs, and provides information on the functional evolution of TLRs. (C) 2012 Elsevier Ltd. All rights reserved.
英文摘要: The mammalian response to lipopolysaccharide (LPS) is mainly mediated by Toll-Like Receptor 4 (TLR4). Fish and mammalian TLR4 vary; fish TLR4 ligands are unknown. Isolation of fish tlr4 genes is difficult due to their complex genomic structure. Three bacterial artificial chromosome (BAC) clones containing grass carp tlr4 were obtained. Four tlr4 genes, with a varied genomic structure and different protein domains were subsequently isolated by constructing a subcloned library and rapid amplification of cDNA ends (RACE). The four tlr4 genes were expressed during development from 12 h post-fertilization, in all healthy adult fish tissues tested, and significantly increased in grass carp reovirus (GCRV)-infected liver and muscle, suggesting the tlr4 genes play a role in GCRV infection. This study effectively separated each gene in the tlr4 gene cluster, implies that grass carp TLR4 proteins have different ligand recognition specificities to mammalian TLRs, and provides information on the functional evolution of TLRs. (C) 2012 Elsevier Ltd. All rights reserved.
关键词[WOS]: RESPIRATORY SYNCYTIAL VIRUS ; INNATE IMMUNITY ; ACTIVATION ; RECOGNITION ; EXPRESSION ; PREDICTION ; EVOLUTION ; FAMILY ; FISH ; LPS
语种: 英语
WOS记录号: WOS:000309312700021
ISSN号: 0145-305X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17226
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Ctr Fish Biol & Fishery Biotechnol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Huang, Rong; Dong, Feng; Jang, Songhun; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping.Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus,DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,38(2):383-388
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