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Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus
Huang, Rong; Dong, Feng2; Jang, Songhun2; Liao, Lanjie; Zhu, Zuoyan; Wang, Yaping1; Wang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Fish Biol & Fishery Biotechnol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
2012-10-01
Source PublicationDEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
ISSN0145-305X
Volume38Issue:2Pages:383-388
AbstractThe mammalian response to lipopolysaccharide (LPS) is mainly mediated by Toll-Like Receptor 4 (TLR4). Fish and mammalian TLR4 vary; fish TLR4 ligands are unknown. Isolation of fish tlr4 genes is difficult due to their complex genomic structure. Three bacterial artificial chromosome (BAC) clones containing grass carp tlr4 were obtained. Four tlr4 genes, with a varied genomic structure and different protein domains were subsequently isolated by constructing a subcloned library and rapid amplification of cDNA ends (RACE). The four tlr4 genes were expressed during development from 12 h post-fertilization, in all healthy adult fish tissues tested, and significantly increased in grass carp reovirus (GCRV)-infected liver and muscle, suggesting the tlr4 genes play a role in GCRV infection. This study effectively separated each gene in the tlr4 gene cluster, implies that grass carp TLR4 proteins have different ligand recognition specificities to mammalian TLRs, and provides information on the functional evolution of TLRs. (C) 2012 Elsevier Ltd. All rights reserved.; The mammalian response to lipopolysaccharide (LPS) is mainly mediated by Toll-Like Receptor 4 (TLR4). Fish and mammalian TLR4 vary; fish TLR4 ligands are unknown. Isolation of fish tlr4 genes is difficult due to their complex genomic structure. Three bacterial artificial chromosome (BAC) clones containing grass carp tlr4 were obtained. Four tlr4 genes, with a varied genomic structure and different protein domains were subsequently isolated by constructing a subcloned library and rapid amplification of cDNA ends (RACE). The four tlr4 genes were expressed during development from 12 h post-fertilization, in all healthy adult fish tissues tested, and significantly increased in grass carp reovirus (GCRV)-infected liver and muscle, suggesting the tlr4 genes play a role in GCRV infection. This study effectively separated each gene in the tlr4 gene cluster, implies that grass carp TLR4 proteins have different ligand recognition specificities to mammalian TLRs, and provides information on the functional evolution of TLRs. (C) 2012 Elsevier Ltd. All rights reserved.
SubtypeArticle
KeywordGrass Carp Bac Library Tlr4 Gene Cluster Grass Carp Reovirus
Department[Wang, Yaping] Chinese Acad Sci, Inst Hydrobiol, Ctr Fish Biol & Fishery Biotechnol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China; [Dong, Feng; Jang, Songhun] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
DOI10.1016/j.dci.2012.06.002
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3] ; National Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3] ; National Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3] ; National Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3]
Indexed BySCI
Language英语
WOS Research AreaImmunology ; Zoology
WOS SubjectImmunology ; Zoology
WOS IDWOS:000309312700021
WOS KeywordRESPIRATORY SYNCYTIAL VIRUS ; INNATE IMMUNITY ; ACTIVATION ; RECOGNITION ; EXPRESSION ; PREDICTION ; EVOLUTION ; FAMILY ; FISH ; LPS
Funding OrganizationNational Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3] ; National Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3] ; National Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3] ; National Basic Research Program of China [2009CB118701]; Chinese Academy of Sciences [KSCX2-EW-N-004-3]
Citation statistics
Cited Times:26[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17226
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, YP (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Fish Biol & Fishery Biotechnol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Ctr Fish Biol & Fishery Biotechnol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Huang, Rong,Dong, Feng,Jang, Songhun,et al. Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2012,38(2):383-388.
APA Huang, Rong.,Dong, Feng.,Jang, Songhun.,Liao, Lanjie.,Zhu, Zuoyan.,...&Wang, YP .(2012).Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,38(2),383-388.
MLA Huang, Rong,et al."Isolation and analysis of a novel grass carp toll-like receptor 4 (tlr4) gene cluster involved in the response to grass carp reovirus".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 38.2(2012):383-388.
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