中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 淡水生态学研究中心  > 期刊论文
题名: TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro
作者: Zhou, Wenshan2; Liang, Hualei2; Xie, Ping1, 2; Zhang, Xuezhen2
通讯作者: Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China.
关键词: Erythrocytes ; lipid peroxidation ; oxidative stress ; in vitro ; haemolysis ; Microcystin-LR
刊名: FRESENIUS ENVIRONMENTAL BULLETIN
发表日期: 2012
卷: 21, 期:8A, 页:2274-2281
收录类别: SCI
文章类型: Article
部门归属: [Xie, Ping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China; [Zhou, Wenshan; Liang, Hualei; Xie, Ping; Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China [30800160]
类目[WOS]: Environmental Sciences
研究领域[WOS]: Environmental Sciences & Ecology
摘要: Haematological abnormalities have been verified in patients intoxicated by microcystins (MCs) in haemodialysis unit in Caruaru, Brazil, and 60 patients died. In our previous studies, obvious anemia has been determined in rabbit after in vivo exposure to microcystins. As to the cause of the anemia, except for hematopoiesis obstacles, we hypothesized that microcystins result in erythrocyte destruction. In the present study, Kunming mice erythrocytes in vitro were incubated with 1, 10, 100 and 1000 nM microcystin-LR at 37 degrees C. Lipid peroxidation, haemolysis, cell morphology, antioxidative response and some biochemical biomarkers were measured. The results showed that the level of lipid peroxidation significantly increased in microcystin-LR treatment groups. The level of glutathione and activities of glutathione peroxidase, glutathione-S-transferase and superoxide dismutase were significantly increased after incubation with microcystin-LR at 12, 24 and 48 h. Also, significant decreases in activities of acetylcholinesterase, Na+-K+-ATPase and Ca2+-Mg2+-ATPase were observed. Obvious increases of haemolysis were determined in 10, 100 and 1000 nM groups from 12 to 48 h. Additionally, abnormal erythrocytes with bleb-bing and notched cell membrane were observed in both 100 and 1000 nM groups. It is presumed that microcystin-LR triggers lipid peroxidation of erythrocytes and oxidative stress destroys the structure of cell membrane, leading to alterations of antioxidative enzymes and biochemical indicators. Our results demonstrate that in vitro exposure to microcystin-LR resulted in damage of mice erythrocytes.
英文摘要: Haematological abnormalities have been verified in patients intoxicated by microcystins (MCs) in haemodialysis unit in Caruaru, Brazil, and 60 patients died. In our previous studies, obvious anemia has been determined in rabbit after in vivo exposure to microcystins. As to the cause of the anemia, except for hematopoiesis obstacles, we hypothesized that microcystins result in erythrocyte destruction. In the present study, Kunming mice erythrocytes in vitro were incubated with 1, 10, 100 and 1000 nM microcystin-LR at 37 degrees C. Lipid peroxidation, haemolysis, cell morphology, antioxidative response and some biochemical biomarkers were measured. The results showed that the level of lipid peroxidation significantly increased in microcystin-LR treatment groups. The level of glutathione and activities of glutathione peroxidase, glutathione-S-transferase and superoxide dismutase were significantly increased after incubation with microcystin-LR at 12, 24 and 48 h. Also, significant decreases in activities of acetylcholinesterase, Na+-K+-ATPase and Ca2+-Mg2+-ATPase were observed. Obvious increases of haemolysis were determined in 10, 100 and 1000 nM groups from 12 to 48 h. Additionally, abnormal erythrocytes with bleb-bing and notched cell membrane were observed in both 100 and 1000 nM groups. It is presumed that microcystin-LR triggers lipid peroxidation of erythrocytes and oxidative stress destroys the structure of cell membrane, leading to alterations of antioxidative enzymes and biochemical indicators. Our results demonstrate that in vitro exposure to microcystin-LR resulted in damage of mice erythrocytes.
关键词[WOS]: OXIDATIVE STRESS RESPONSES ; CULTURED RAT HEPATOCYTES ; LIPID-PEROXIDATION ; FREE-RADICALS ; DAMAGE ; EXPOSURE ; LIVER ; EXTRACTS ; ACETYLCHOLINESTERASE ; NA+,K+-ATPASE
语种: 英语
WOS记录号: WOS:000309326100017
ISSN号: 1018-4619
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17194
Appears in Collections:淡水生态学研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro.pdf(2121KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China

Recommended Citation:
Zhou, Wenshan; Liang, Hualei; Xie, Ping; Zhang, Xuezhen.TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro,FRESENIUS ENVIRONMENTAL BULLETIN,2012,21(8A):2274-2281
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhou, Wenshan]'s Articles
[Liang, Hualei]'s Articles
[Xie, Ping]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhou, Wenshan]‘s Articles
[Liang, Hualei]‘s Articles
[Xie, Ping]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace