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TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro
Zhou, Wenshan2; Liang, Hualei2; Xie, Ping1,2; Zhang, Xuezhen2; Xie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China.
2012
Source PublicationFRESENIUS ENVIRONMENTAL BULLETIN
ISSN1018-4619
Volume21Issue:8APages:2274-2281
AbstractHaematological abnormalities have been verified in patients intoxicated by microcystins (MCs) in haemodialysis unit in Caruaru, Brazil, and 60 patients died. In our previous studies, obvious anemia has been determined in rabbit after in vivo exposure to microcystins. As to the cause of the anemia, except for hematopoiesis obstacles, we hypothesized that microcystins result in erythrocyte destruction. In the present study, Kunming mice erythrocytes in vitro were incubated with 1, 10, 100 and 1000 nM microcystin-LR at 37 degrees C. Lipid peroxidation, haemolysis, cell morphology, antioxidative response and some biochemical biomarkers were measured. The results showed that the level of lipid peroxidation significantly increased in microcystin-LR treatment groups. The level of glutathione and activities of glutathione peroxidase, glutathione-S-transferase and superoxide dismutase were significantly increased after incubation with microcystin-LR at 12, 24 and 48 h. Also, significant decreases in activities of acetylcholinesterase, Na+-K+-ATPase and Ca2+-Mg2+-ATPase were observed. Obvious increases of haemolysis were determined in 10, 100 and 1000 nM groups from 12 to 48 h. Additionally, abnormal erythrocytes with bleb-bing and notched cell membrane were observed in both 100 and 1000 nM groups. It is presumed that microcystin-LR triggers lipid peroxidation of erythrocytes and oxidative stress destroys the structure of cell membrane, leading to alterations of antioxidative enzymes and biochemical indicators. Our results demonstrate that in vitro exposure to microcystin-LR resulted in damage of mice erythrocytes.; Haematological abnormalities have been verified in patients intoxicated by microcystins (MCs) in haemodialysis unit in Caruaru, Brazil, and 60 patients died. In our previous studies, obvious anemia has been determined in rabbit after in vivo exposure to microcystins. As to the cause of the anemia, except for hematopoiesis obstacles, we hypothesized that microcystins result in erythrocyte destruction. In the present study, Kunming mice erythrocytes in vitro were incubated with 1, 10, 100 and 1000 nM microcystin-LR at 37 degrees C. Lipid peroxidation, haemolysis, cell morphology, antioxidative response and some biochemical biomarkers were measured. The results showed that the level of lipid peroxidation significantly increased in microcystin-LR treatment groups. The level of glutathione and activities of glutathione peroxidase, glutathione-S-transferase and superoxide dismutase were significantly increased after incubation with microcystin-LR at 12, 24 and 48 h. Also, significant decreases in activities of acetylcholinesterase, Na+-K+-ATPase and Ca2+-Mg2+-ATPase were observed. Obvious increases of haemolysis were determined in 10, 100 and 1000 nM groups from 12 to 48 h. Additionally, abnormal erythrocytes with bleb-bing and notched cell membrane were observed in both 100 and 1000 nM groups. It is presumed that microcystin-LR triggers lipid peroxidation of erythrocytes and oxidative stress destroys the structure of cell membrane, leading to alterations of antioxidative enzymes and biochemical indicators. Our results demonstrate that in vitro exposure to microcystin-LR resulted in damage of mice erythrocytes.
SubtypeArticle
KeywordErythrocytes Lipid Peroxidation Oxidative Stress In Vitro Haemolysis Microcystin-lr
Department[Xie, Ping] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China; [Zhou, Wenshan; Liang, Hualei; Xie, Ping; Zhang, Xuezhen] Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000309326100017
WOS KeywordOXIDATIVE STRESS RESPONSES ; CULTURED RAT HEPATOCYTES ; LIPID-PEROXIDATION ; FREE-RADICALS ; DAMAGE ; EXPOSURE ; LIVER ; EXTRACTS ; ACETYLCHOLINESTERASE ; NA+,K+-ATPASE
Funding OrganizationNational Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160] ; National Natural Science Foundation of China [30800160]
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17194
Collection淡水生态学研究中心_期刊论文
Corresponding AuthorXie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China
Recommended Citation
GB/T 7714
Zhou, Wenshan,Liang, Hualei,Xie, Ping,et al. TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2012,21(8A):2274-2281.
APA Zhou, Wenshan,Liang, Hualei,Xie, Ping,Zhang, Xuezhen,&Xie, P .(2012).TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro.FRESENIUS ENVIRONMENTAL BULLETIN,21(8A),2274-2281.
MLA Zhou, Wenshan,et al."TOXIC EFFECTS OF MICROCYSTIN-LR ON MICE ERYTHROCYTES in vitro".FRESENIUS ENVIRONMENTAL BULLETIN 21.8A(2012):2274-2281.
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