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Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses
Chen, Hui; Chen, Shan-Li; Jiang, Jian-Guo; Chen, H (reprint author), S China Univ Technol, Coll Food & Bioengn, Guangzhou, Guangdong, Peoples R China.
2011
Source PublicationPLOS ONE
ISSN1932-6203
Volume6Issue:12Pages:e28613
AbstractThe effect of Ca2+ channel blockers on cytosolic Ca2+ levels and the role of Ca2+ in glycerol metabolism of Dunaliella salina under hypoosmotic or hyperosmotic stress were investigated using the confocal laser scanning microscope (CLSM). Results showed that intracellular Ca2+ concentration increased rapidly when extracellular salinity suddenly decreased or increased, but the increase could be inhibited by pretreatment of Ca2+ channel blockers LaCl3, verapamil or ruthenium red. The changes of glycerol content and G3pdh activity in D. salina to respect to hypoosmotic or hyperosmotic stress were also inhibited in different degrees by pretreatment of Ca2+ channel blockers, indicating that the influx of Ca2+ via Ca2+ channels are required for the transduction of osmotic signal to regulate osmotic responses of D. salina to the changes of salinity. Differences of the three blockers in block effect suggested that they may act on different channels or had different action sites, including influx of Ca2+ from the extracellular space via Ca2+ channels localized in the plasma membrane or from intracellular calcium store via the mitochondrial. Other Ca2+-mediated or non-Ca2+-mediated osmotic signal pathway may exist in Dunaliella in response to hypoosmotic and hyperosmotic stresses.
KeywordGlycerol-3-phosphate Dehydrogenase Beta-carotene Algal Cells Calcium Arabidopsis Biosynthesis Expression Proteins Signals Cloning
Department[Chen, Hui; Chen, Shan-Li; Jiang, Jian-Guo] S China Univ Technol, Coll Food & Bioengn, Guangzhou, Guangdong, Peoples R China; [Chen, Hui] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
Funding OrganizationNational Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025]
Indexed BySCI
Language英语
WOS IDWOS:000298369100063
Funding OrganizationNational Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025]
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17063
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorChen, H (reprint author), S China Univ Technol, Coll Food & Bioengn, Guangzhou, Guangdong, Peoples R China.
Recommended Citation
GB/T 7714
Chen, Hui,Chen, Shan-Li,Jiang, Jian-Guo,et al. Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses[J]. PLOS ONE,2011,6(12):e28613.
APA Chen, Hui,Chen, Shan-Li,Jiang, Jian-Guo,&Chen, H .(2011).Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses.PLOS ONE,6(12),e28613.
MLA Chen, Hui,et al."Effect of Ca2+ Channel Block on Glycerol Metabolism in Dunaliella salina under Hypoosmotic and Hyperosmotic Stresses".PLOS ONE 6.12(2011):e28613.
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