中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 藻类生物学及应用研究中心  > 期刊论文
题名: Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata
作者: Jiang, Yongguang1, 2; Xiao, Peng1, 2; Yu, Gongliang1; Sano, Tomoharu3; Pan, Qianqian1, 2; Li, Renhui1
通讯作者: Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China.
刊名: APPLIED AND ENVIRONMENTAL MICROBIOLOGY
发表日期: 2012-04-01
DOI: 10.1128/AEM.07321-11
卷: 78, 期:7, 页:2256-2263
收录类别: SCI
文章类型: Article
部门归属: [Jiang, Yongguang; Xiao, Peng; Yu, Gongliang; Pan, Qianqian; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; [Jiang, Yongguang; Xiao, Peng; Pan, Qianqian] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Sano, Tomoharu] Natl Inst Environm Studies, Tsukuba, Ibaraki, Japan
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: State Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501]
类目[WOS]: Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Microbiology
摘要: New insights into the distribution and biochemistry of the cyanotoxin cylindrospermopsin (CYN) have been provided by the recent determination of its biosynthesis gene cluster (cyr) in several cyanobacterial species. Raphidiopsis curvata CHAB1150 isolated from China was analyzed for CYN analogues. Only 7-deoxy-CYN was detected in the cell extracts. The cyr gene cluster of R. curvata CHAB1150 was sequenced, and the cyr genes of this strain were found to have extremely high similarities (96% to 100%) to those from other nostocalean species. These species include Cylindrospermopsis raciborskii AWT205, Aphanizomenon sp. strain 10E6, and Aphanizomenon ovalisporum ILC-146. Insertion mutation was identified within the cyrI gene, and transcripts of cyrI and another functional gene cyrJ were detected in R. curvata CHAB1150. General congruence between the phylogenetic trees based on both cyr and 16S rrn was displayed. Neutral evolution was found on the whole sequences of the cyr genes, and 0 to 89 negative selected codons were detected in each gene. Therefore, the function of CyrI is to catalyze the oxygenation of 7-deoxy-CYN in CYN biosynthesis. The transcripts of the mutated cyrI gene may result from polycistronic transcription. The high conservation of the cyr genes may be ascribed to purifying selection and horizontal gene transfer.
英文摘要: New insights into the distribution and biochemistry of the cyanotoxin cylindrospermopsin (CYN) have been provided by the recent determination of its biosynthesis gene cluster (cyr) in several cyanobacterial species. Raphidiopsis curvata CHAB1150 isolated from China was analyzed for CYN analogues. Only 7-deoxy-CYN was detected in the cell extracts. The cyr gene cluster of R. curvata CHAB1150 was sequenced, and the cyr genes of this strain were found to have extremely high similarities (96% to 100%) to those from other nostocalean species. These species include Cylindrospermopsis raciborskii AWT205, Aphanizomenon sp. strain 10E6, and Aphanizomenon ovalisporum ILC-146. Insertion mutation was identified within the cyrI gene, and transcripts of cyrI and another functional gene cyrJ were detected in R. curvata CHAB1150. General congruence between the phylogenetic trees based on both cyr and 16S rrn was displayed. Neutral evolution was found on the whole sequences of the cyr genes, and 0 to 89 negative selected codons were detected in each gene. Therefore, the function of CyrI is to catalyze the oxygenation of 7-deoxy-CYN in CYN biosynthesis. The transcripts of the mutated cyrI gene may result from polycistronic transcription. The high conservation of the cyr genes may be ascribed to purifying selection and horizontal gene transfer.
关键词[WOS]: 2 GERMAN LAKES ; CYLINDROSPERMOPSIS-RACIBORSKII ; GENE-CLUSTER ; CYANOTOXINS CYLINDROSPERMOPSIN ; APHANIZOMENON-OVALISPORUM ; PROTEIN-SYNTHESIS ; UMEZAKIA-NATANS ; 1ST REPORT ; IDENTIFICATION ; STRAIN
语种: 英语
WOS记录号: WOS:000301344300021
ISSN号: 0099-2240
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17057
Appears in Collections:藻类生物学及应用研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata.pdf(1635KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Natl Inst Environm Studies, Tsukuba, Ibaraki, Japan

Recommended Citation:
Jiang, Yongguang; Xiao, Peng; Yu, Gongliang; Sano, Tomoharu; Pan, Qianqian; Li, Renhui.Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata,APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2012,78(7):2256-2263
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Jiang, Yongguang]'s Articles
[Xiao, Peng]'s Articles
[Yu, Gongliang]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Jiang, Yongguang]‘s Articles
[Xiao, Peng]‘s Articles
[Yu, Gongliang]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院水生生物研究所 - Feedback
Powered by CSpace