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Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata
Jiang, Yongguang1,2; Xiao, Peng1,2; Yu, Gongliang1; Sano, Tomoharu3; Pan, Qianqian1,2; Li, Renhui1; Li, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China.
2012-04-01
Source PublicationAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN0099-2240
Volume78Issue:7Pages:2256-2263
AbstractNew insights into the distribution and biochemistry of the cyanotoxin cylindrospermopsin (CYN) have been provided by the recent determination of its biosynthesis gene cluster (cyr) in several cyanobacterial species. Raphidiopsis curvata CHAB1150 isolated from China was analyzed for CYN analogues. Only 7-deoxy-CYN was detected in the cell extracts. The cyr gene cluster of R. curvata CHAB1150 was sequenced, and the cyr genes of this strain were found to have extremely high similarities (96% to 100%) to those from other nostocalean species. These species include Cylindrospermopsis raciborskii AWT205, Aphanizomenon sp. strain 10E6, and Aphanizomenon ovalisporum ILC-146. Insertion mutation was identified within the cyrI gene, and transcripts of cyrI and another functional gene cyrJ were detected in R. curvata CHAB1150. General congruence between the phylogenetic trees based on both cyr and 16S rrn was displayed. Neutral evolution was found on the whole sequences of the cyr genes, and 0 to 89 negative selected codons were detected in each gene. Therefore, the function of CyrI is to catalyze the oxygenation of 7-deoxy-CYN in CYN biosynthesis. The transcripts of the mutated cyrI gene may result from polycistronic transcription. The high conservation of the cyr genes may be ascribed to purifying selection and horizontal gene transfer.; New insights into the distribution and biochemistry of the cyanotoxin cylindrospermopsin (CYN) have been provided by the recent determination of its biosynthesis gene cluster (cyr) in several cyanobacterial species. Raphidiopsis curvata CHAB1150 isolated from China was analyzed for CYN analogues. Only 7-deoxy-CYN was detected in the cell extracts. The cyr gene cluster of R. curvata CHAB1150 was sequenced, and the cyr genes of this strain were found to have extremely high similarities (96% to 100%) to those from other nostocalean species. These species include Cylindrospermopsis raciborskii AWT205, Aphanizomenon sp. strain 10E6, and Aphanizomenon ovalisporum ILC-146. Insertion mutation was identified within the cyrI gene, and transcripts of cyrI and another functional gene cyrJ were detected in R. curvata CHAB1150. General congruence between the phylogenetic trees based on both cyr and 16S rrn was displayed. Neutral evolution was found on the whole sequences of the cyr genes, and 0 to 89 negative selected codons were detected in each gene. Therefore, the function of CyrI is to catalyze the oxygenation of 7-deoxy-CYN in CYN biosynthesis. The transcripts of the mutated cyrI gene may result from polycistronic transcription. The high conservation of the cyr genes may be ascribed to purifying selection and horizontal gene transfer.
SubtypeArticle
Department[Jiang, Yongguang; Xiao, Peng; Yu, Gongliang; Pan, Qianqian; Li, Renhui] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; [Jiang, Yongguang; Xiao, Peng; Pan, Qianqian] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China; [Sano, Tomoharu] Natl Inst Environm Studies, Tsukuba, Ibaraki, Japan
DOI10.1128/AEM.07321-11
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationState Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501]
Indexed BySCI
Language英语
WOS Research AreaBiotechnology & Applied Microbiology ; Microbiology
WOS SubjectBiotechnology & Applied Microbiology ; Microbiology
WOS IDWOS:000301344300021
WOS Keyword2 GERMAN LAKES ; CYLINDROSPERMOPSIS-RACIBORSKII ; GENE-CLUSTER ; CYANOTOXINS CYLINDROSPERMOPSIN ; APHANIZOMENON-OVALISPORUM ; PROTEIN-SYNTHESIS ; UMEZAKIA-NATANS ; 1ST REPORT ; IDENTIFICATION ; STRAIN
Funding OrganizationState Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501] ; State Key Basic Research and Development Plan of China[2008CB418002]; National Natural Science Foundation of China[31170189]; National Water Science and Technology Projects[2009ZX07101-013-02]; Chinese Academy of Sciences[082303-1-501]
Citation statistics
Cited Times:35[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17057
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorLi, RH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
3.Natl Inst Environm Studies, Tsukuba, Ibaraki, Japan
Recommended Citation
GB/T 7714
Jiang, Yongguang,Xiao, Peng,Yu, Gongliang,et al. Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2012,78(7):2256-2263.
APA Jiang, Yongguang.,Xiao, Peng.,Yu, Gongliang.,Sano, Tomoharu.,Pan, Qianqian.,...&Li, RH .(2012).Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,78(7),2256-2263.
MLA Jiang, Yongguang,et al."Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 78.7(2012):2256-2263.
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