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Identification of the HetR Recognition Sequence Upstream of hetZ in Anabaena sp Strain PCC 7120
Du, Ye; Cai, Yan; Hou, Shengwei; Xu, Xudong; Xu, XD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China.
2012-05-01
Source PublicationJOURNAL OF BACTERIOLOGY
ISSN0021-9193
Volume194Issue:9Pages:2297-2306
AbstractHetR is the master regulator of heterocyst differentiation in Anabaena sp. strain PCC 7120 and has been found to specifically bind to an inverted-repeat-containing region upstream of hetP, a heterocyst differentiation gene. However, no such inverted-repeat sequence can be found in promoters of other genes in the genome. hetZ is a gene involved in early heterocyst differentiation. As shown with the gfp reporter gene, transcription from P-hetZ was correlated to the expression level of hetR and inhibition by RGSGR, the pentapeptide derived from the C terminus of PatS. As detected by electrophoretic mobility shift assay, a recombinant HetR showed specific binding to the region upstream of hetZ, and the binding was inhibited by RGSGR. Tests of a series of the upstream fragments delimited the HetR-binding site to a 40-bp region that shows similarity to that upstream of hetP. The introduction of substitutions of bases conserved in the two HetR-binding sites showed that at least 12 bases are required for recognition by HetR. Deletion of a 51-bp region containing the HetR-binding site completely eliminated the transcription activity of P-hetZ. Based on the HetR recognition sequence of hetZ, those upstream of hetR and patA are proposed.; HetR is the master regulator of heterocyst differentiation in Anabaena sp. strain PCC 7120 and has been found to specifically bind to an inverted-repeat-containing region upstream of hetP, a heterocyst differentiation gene. However, no such inverted-repeat sequence can be found in promoters of other genes in the genome. hetZ is a gene involved in early heterocyst differentiation. As shown with the gfp reporter gene, transcription from P-hetZ was correlated to the expression level of hetR and inhibition by RGSGR, the pentapeptide derived from the C terminus of PatS. As detected by electrophoretic mobility shift assay, a recombinant HetR showed specific binding to the region upstream of hetZ, and the binding was inhibited by RGSGR. Tests of a series of the upstream fragments delimited the HetR-binding site to a 40-bp region that shows similarity to that upstream of hetP. The introduction of substitutions of bases conserved in the two HetR-binding sites showed that at least 12 bases are required for recognition by HetR. Deletion of a 51-bp region containing the HetR-binding site completely eliminated the transcription activity of P-hetZ. Based on the HetR recognition sequence of hetZ, those upstream of hetR and patA are proposed.
SubtypeArticle
KeywordHeterocyst Pattern-formation Escherichia-coli Sp Pcc-7120 Gene-product Differentiation Expression Cyanobacteria Pata Proteins Mutation
Department[Du, Ye; Cai, Yan; Hou, Shengwei; Xu, Xudong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
DOI10.1128/JB.00119-12
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China[30770049, 30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30770049, 30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30770049, 30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30770049, 30825003]; State Key Basic Research Development Program of China[2008CB418001]
Indexed BySCI
Language英语
WOS Research AreaMicrobiology
WOS SubjectMicrobiology
WOS IDWOS:000302808400018
WOS KeywordHETEROCYST PATTERN-FORMATION ; ESCHERICHIA-COLI ; SP PCC-7120 ; GENE-PRODUCT ; DIFFERENTIATION ; EXPRESSION ; CYANOBACTERIA ; PATA ; PROTEINS ; MUTATION
Funding OrganizationNational Natural Science Foundation of China[30770049, 30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30770049, 30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30770049, 30825003]; State Key Basic Research Development Program of China[2008CB418001] ; National Natural Science Foundation of China[30770049, 30825003]; State Key Basic Research Development Program of China[2008CB418001]
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Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17047
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, XD (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China.
AffiliationChinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Du, Ye,Cai, Yan,Hou, Shengwei,et al. Identification of the HetR Recognition Sequence Upstream of hetZ in Anabaena sp Strain PCC 7120[J]. JOURNAL OF BACTERIOLOGY,2012,194(9):2297-2306.
APA Du, Ye,Cai, Yan,Hou, Shengwei,Xu, Xudong,&Xu, XD .(2012).Identification of the HetR Recognition Sequence Upstream of hetZ in Anabaena sp Strain PCC 7120.JOURNAL OF BACTERIOLOGY,194(9),2297-2306.
MLA Du, Ye,et al."Identification of the HetR Recognition Sequence Upstream of hetZ in Anabaena sp Strain PCC 7120".JOURNAL OF BACTERIOLOGY 194.9(2012):2297-2306.
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