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Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp NJ-18
Wang, Xin; Xu, Xudong; Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
2012-06-10
Source PublicationGENE
ISSN0378-1119
Volume501Issue:1Pages:17-23
AbstractPhotosynthetic organisms often encounter oxidative stresses due to changes of environmental conditions. In this study, two glutathione peroxidase (GPX) homologous genes, namely NJ-18Gpx1 and NJ-18Gpx2, were identified in Chlorella sp. NJ-18, a single-celled green alga. The two NJ-18Gpx genes can produce 2 or 3 transcript variants by alternative splicing, predicted to encode 4 non-selenium GPX proteins (NS-GPX). Expression of the two genes was analyzed by quantitative RT-PCR in Chlorella sp. NJ-18 exposed to various treatments known to generate reactive oxygen species. Neutral red, a singlet oxygen-generating photosensitizer, significantly increased the expression of NJ-18Gpx1 within 5 h. Exposure of algal culture to UV-B for 3 h caused up-regulation of mRNA levels of NJ-18Gpx1 and NJ-18Gpx2 by 4- and 50-folds, respectively. Similar to CrGPX5 and CrGPX3 in Chlamydomonas reinhardtii, purified recombinant NJ-18GPXs showed activities of thioredoxin-dependent peroxidases that catalyze the reduction of hydrogen peroxide and organic hydroperoxides. The V-max values for NJ-18GPX1 toward different peroxides were approximately 10-fold higher than those for NJ-18GPX2. In addition, overexpression of NJ-18Gpx1 in Synechocystis sp. PCC 6803, a cyanobacterium, enhanced its tolerance to neutral red and H2O2. These results indicate that NJ-18GPXs can act as efficient peroxide scavengers protecting cells from oxidative damages in Chlorella. (C) 2012 Elsevier B.V. All rights reserved.; Photosynthetic organisms often encounter oxidative stresses due to changes of environmental conditions. In this study, two glutathione peroxidase (GPX) homologous genes, namely NJ-18Gpx1 and NJ-18Gpx2, were identified in Chlorella sp. NJ-18, a single-celled green alga. The two NJ-18Gpx genes can produce 2 or 3 transcript variants by alternative splicing, predicted to encode 4 non-selenium GPX proteins (NS-GPX). Expression of the two genes was analyzed by quantitative RT-PCR in Chlorella sp. NJ-18 exposed to various treatments known to generate reactive oxygen species. Neutral red, a singlet oxygen-generating photosensitizer, significantly increased the expression of NJ-18Gpx1 within 5 h. Exposure of algal culture to UV-B for 3 h caused up-regulation of mRNA levels of NJ-18Gpx1 and NJ-18Gpx2 by 4- and 50-folds, respectively. Similar to CrGPX5 and CrGPX3 in Chlamydomonas reinhardtii, purified recombinant NJ-18GPXs showed activities of thioredoxin-dependent peroxidases that catalyze the reduction of hydrogen peroxide and organic hydroperoxides. The V-max values for NJ-18GPX1 toward different peroxides were approximately 10-fold higher than those for NJ-18GPX2. In addition, overexpression of NJ-18Gpx1 in Synechocystis sp. PCC 6803, a cyanobacterium, enhanced its tolerance to neutral red and H2O2. These results indicate that NJ-18GPXs can act as efficient peroxide scavengers protecting cells from oxidative damages in Chlorella. (C) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordThioredoxin-dependent Gpx Induced Expression Chlorella Synechocystis
Department[Wang, Xin; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
DOI10.1016/j.gene.2012.04.003
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationChinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity
WOS SubjectGenetics & Heredity
WOS IDWOS:000304584000003
WOS KeywordSYNECHOCYSTIS SP PCC-6803 ; CHLAMYDOMONAS-REINHARDTII ; OXIDATIVE STRESS ; ARABIDOPSIS-THALIANA ; ANTIOXIDANT ENZYMES ; ABIOTIC STRESSES ; REDOX-TRANSDUCER ; SINGLET OXYGEN ; FAMILY ; PEROXIREDOXIN
Funding OrganizationChinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332] ; Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17041
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorXu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
Recommended Citation
GB/T 7714
Wang, Xin,Xu, Xudong,Xu, XD . Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp NJ-18[J]. GENE,2012,501(1):17-23.
APA Wang, Xin,Xu, Xudong,&Xu, XD .(2012).Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp NJ-18.GENE,501(1),17-23.
MLA Wang, Xin,et al."Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp NJ-18".GENE 501.1(2012):17-23.
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