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题名: Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp NJ-18
作者: Wang, Xin1; Xu, Xudong1
通讯作者: Xu, XD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China.
关键词: Thioredoxin-dependent GPX ; Induced expression ; Chlorella ; Synechocystis
刊名: GENE
发表日期: 2012-06-10
DOI: 10.1016/j.gene.2012.04.003
卷: 501, 期:1, 页:17-23
收录类别: SCI
文章类型: Article
部门归属: [Wang, Xin; Xu, Xudong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Chinese Academy of Sciences[KSCX2-YW-G-060, KSCX2-SW-332]
类目[WOS]: Genetics & Heredity
研究领域[WOS]: Genetics & Heredity
摘要: Photosynthetic organisms often encounter oxidative stresses due to changes of environmental conditions. In this study, two glutathione peroxidase (GPX) homologous genes, namely NJ-18Gpx1 and NJ-18Gpx2, were identified in Chlorella sp. NJ-18, a single-celled green alga. The two NJ-18Gpx genes can produce 2 or 3 transcript variants by alternative splicing, predicted to encode 4 non-selenium GPX proteins (NS-GPX). Expression of the two genes was analyzed by quantitative RT-PCR in Chlorella sp. NJ-18 exposed to various treatments known to generate reactive oxygen species. Neutral red, a singlet oxygen-generating photosensitizer, significantly increased the expression of NJ-18Gpx1 within 5 h. Exposure of algal culture to UV-B for 3 h caused up-regulation of mRNA levels of NJ-18Gpx1 and NJ-18Gpx2 by 4- and 50-folds, respectively. Similar to CrGPX5 and CrGPX3 in Chlamydomonas reinhardtii, purified recombinant NJ-18GPXs showed activities of thioredoxin-dependent peroxidases that catalyze the reduction of hydrogen peroxide and organic hydroperoxides. The V-max values for NJ-18GPX1 toward different peroxides were approximately 10-fold higher than those for NJ-18GPX2. In addition, overexpression of NJ-18Gpx1 in Synechocystis sp. PCC 6803, a cyanobacterium, enhanced its tolerance to neutral red and H2O2. These results indicate that NJ-18GPXs can act as efficient peroxide scavengers protecting cells from oxidative damages in Chlorella. (C) 2012 Elsevier B.V. All rights reserved.
英文摘要: Photosynthetic organisms often encounter oxidative stresses due to changes of environmental conditions. In this study, two glutathione peroxidase (GPX) homologous genes, namely NJ-18Gpx1 and NJ-18Gpx2, were identified in Chlorella sp. NJ-18, a single-celled green alga. The two NJ-18Gpx genes can produce 2 or 3 transcript variants by alternative splicing, predicted to encode 4 non-selenium GPX proteins (NS-GPX). Expression of the two genes was analyzed by quantitative RT-PCR in Chlorella sp. NJ-18 exposed to various treatments known to generate reactive oxygen species. Neutral red, a singlet oxygen-generating photosensitizer, significantly increased the expression of NJ-18Gpx1 within 5 h. Exposure of algal culture to UV-B for 3 h caused up-regulation of mRNA levels of NJ-18Gpx1 and NJ-18Gpx2 by 4- and 50-folds, respectively. Similar to CrGPX5 and CrGPX3 in Chlamydomonas reinhardtii, purified recombinant NJ-18GPXs showed activities of thioredoxin-dependent peroxidases that catalyze the reduction of hydrogen peroxide and organic hydroperoxides. The V-max values for NJ-18GPX1 toward different peroxides were approximately 10-fold higher than those for NJ-18GPX2. In addition, overexpression of NJ-18Gpx1 in Synechocystis sp. PCC 6803, a cyanobacterium, enhanced its tolerance to neutral red and H2O2. These results indicate that NJ-18GPXs can act as efficient peroxide scavengers protecting cells from oxidative damages in Chlorella. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: SYNECHOCYSTIS SP PCC-6803 ; CHLAMYDOMONAS-REINHARDTII ; OXIDATIVE STRESS ; ARABIDOPSIS-THALIANA ; ANTIOXIDANT ENZYMES ; ABIOTIC STRESSES ; REDOX-TRANSDUCER ; SINGLET OXYGEN ; FAMILY ; PEROXIREDOXIN
语种: 英语
WOS记录号: WOS:000304584000003
ISSN号: 0378-1119
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17041
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China

Recommended Citation:
Wang, Xin; Xu, Xudong.Molecular cloning and functional analyses of glutathione peroxidase homologous genes from Chlorella sp NJ-18,GENE,2012,501(1):17-23
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