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Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses
Chen, Hui1,2; Lu, Yan3; Jiang, Jian-Guo1; Chen, H (reprint author), S China Univ Technol, Coll Food Bioengn, Guangzhou, Guangdong, Peoples R China.
2012-06-04
Source PublicationPLOS ONE
ISSN1932-6203
Volume7Issue:6Pages:e37578
AbstractThe glycerol metabolic pathway is a special cycle way; glycerol-3-phosphate dehydrogenase (G3pdh), glycerol-3-phosphate phosphatase (G3pp), dihydroxyacetone reductase (Dhar), and dihydroxyacetone kinase (Dhak) are the key enzymes around the pathway. Glycerol is an important osmolyte for Dunaliella salina to resist osmotic stress. In this study, comparative activities of the four enzymes in D. salina and their activity changes under various salt stresses were investigated, from which glycerol metabolic flow direction in the glycerol metabolic pathway was estimated. Results showed that the salinity changes had different effects on the enzymes activities. NaCl could stimulate the activities of all the four enzymes in various degrees when D. salina was grown under continuous salt stress. When treated by hyperosmotic or hypoosmotic shock, only the activity of G3pdh in D. salina was significantly stimulated. It was speculated that, under osmotic stresses, the emergency response of the cycle pathway in D. salina was driven by G3pdh via its response to the osmotic stress. Subsequently, with the changes of salinity, other three enzymes started to respond to osmotic stress. Dhar played a role of balancing the cycle metabolic pathway by its forward and backward reactions. Through synergy, the four enzymes worked together for the effective flow of the cycle metabolic pathways to maintain the glycerol requirements of cells in order to adapt to osmotic stress environments.; The glycerol metabolic pathway is a special cycle way; glycerol-3-phosphate dehydrogenase (G3pdh), glycerol-3-phosphate phosphatase (G3pp), dihydroxyacetone reductase (Dhar), and dihydroxyacetone kinase (Dhak) are the key enzymes around the pathway. Glycerol is an important osmolyte for Dunaliella salina to resist osmotic stress. In this study, comparative activities of the four enzymes in D. salina and their activity changes under various salt stresses were investigated, from which glycerol metabolic flow direction in the glycerol metabolic pathway was estimated. Results showed that the salinity changes had different effects on the enzymes activities. NaCl could stimulate the activities of all the four enzymes in various degrees when D. salina was grown under continuous salt stress. When treated by hyperosmotic or hypoosmotic shock, only the activity of G3pdh in D. salina was significantly stimulated. It was speculated that, under osmotic stresses, the emergency response of the cycle pathway in D. salina was driven by G3pdh via its response to the osmotic stress. Subsequently, with the changes of salinity, other three enzymes started to respond to osmotic stress. Dhar played a role of balancing the cycle metabolic pathway by its forward and backward reactions. Through synergy, the four enzymes worked together for the effective flow of the cycle metabolic pathways to maintain the glycerol requirements of cells in order to adapt to osmotic stress environments.
SubtypeArticle
KeywordGlycerol-3-phosphate Dehydrogenase Klebsiella-pneumoniae Beta-carotene Purification Fermentation Tertiolecta Reductase Cloning
Department[Chen, Hui; Jiang, Jian-Guo] S China Univ Technol, Coll Food Bioengn, Guangzhou, Guangdong, Peoples R China; [Chen, Hui] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; [Lu, Yan] S China Univ Technol, Sch Biol Sci & Engn, Guangzhou, Guangdong, Peoples R China
DOI10.1371/journal.pone.0037578
WOS HeadingsScience & Technology
Funding OrganizationNational Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000305341700017
WOS KeywordGLYCEROL-3-PHOSPHATE DEHYDROGENASE ; KLEBSIELLA-PNEUMONIAE ; BETA-CAROTENE ; PURIFICATION ; FERMENTATION ; TERTIOLECTA ; REDUCTASE ; CLONING
Funding OrganizationNational Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025] ; National Natural Foundation of China[30870025]
Citation statistics
Cited Times:27[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17039
Collection藻类生物学及应用研究中心_期刊论文
Corresponding AuthorChen, H (reprint author), S China Univ Technol, Coll Food Bioengn, Guangzhou, Guangdong, Peoples R China.
Affiliation1.S China Univ Technol, Coll Food Bioengn, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
3.S China Univ Technol, Sch Biol Sci & Engn, Guangzhou, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Chen, Hui,Lu, Yan,Jiang, Jian-Guo,et al. Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses[J]. PLOS ONE,2012,7(6):e37578.
APA Chen, Hui,Lu, Yan,Jiang, Jian-Guo,&Chen, H .(2012).Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses.PLOS ONE,7(6),e37578.
MLA Chen, Hui,et al."Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses".PLOS ONE 7.6(2012):e37578.
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