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题名: Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses
作者: Chen, Hui1, 2; Lu, Yan3; Jiang, Jian-Guo1
通讯作者: Chen, H (reprint author), S China Univ Technol, Coll Food Bioengn, Guangzhou, Guangdong, Peoples R China.
关键词: GLYCEROL-3-PHOSPHATE DEHYDROGENASE ; KLEBSIELLA-PNEUMONIAE ; BETA-CAROTENE ; PURIFICATION ; FERMENTATION ; TERTIOLECTA ; REDUCTASE ; CLONING
刊名: PLOS ONE
发表日期: 2012-06-04
DOI: 10.1371/journal.pone.0037578
卷: 7, 期:6, 页:e37578
收录类别: SCI
文章类型: Article
部门归属: [Chen, Hui; Jiang, Jian-Guo] S China Univ Technol, Coll Food Bioengn, Guangzhou, Guangdong, Peoples R China; [Chen, Hui] Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China; [Lu, Yan] S China Univ Technol, Sch Biol Sci & Engn, Guangzhou, Guangdong, Peoples R China
WOS标题词: Science & Technology
资助者: National Natural Foundation of China[30870025]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: The glycerol metabolic pathway is a special cycle way; glycerol-3-phosphate dehydrogenase (G3pdh), glycerol-3-phosphate phosphatase (G3pp), dihydroxyacetone reductase (Dhar), and dihydroxyacetone kinase (Dhak) are the key enzymes around the pathway. Glycerol is an important osmolyte for Dunaliella salina to resist osmotic stress. In this study, comparative activities of the four enzymes in D. salina and their activity changes under various salt stresses were investigated, from which glycerol metabolic flow direction in the glycerol metabolic pathway was estimated. Results showed that the salinity changes had different effects on the enzymes activities. NaCl could stimulate the activities of all the four enzymes in various degrees when D. salina was grown under continuous salt stress. When treated by hyperosmotic or hypoosmotic shock, only the activity of G3pdh in D. salina was significantly stimulated. It was speculated that, under osmotic stresses, the emergency response of the cycle pathway in D. salina was driven by G3pdh via its response to the osmotic stress. Subsequently, with the changes of salinity, other three enzymes started to respond to osmotic stress. Dhar played a role of balancing the cycle metabolic pathway by its forward and backward reactions. Through synergy, the four enzymes worked together for the effective flow of the cycle metabolic pathways to maintain the glycerol requirements of cells in order to adapt to osmotic stress environments.
英文摘要: The glycerol metabolic pathway is a special cycle way; glycerol-3-phosphate dehydrogenase (G3pdh), glycerol-3-phosphate phosphatase (G3pp), dihydroxyacetone reductase (Dhar), and dihydroxyacetone kinase (Dhak) are the key enzymes around the pathway. Glycerol is an important osmolyte for Dunaliella salina to resist osmotic stress. In this study, comparative activities of the four enzymes in D. salina and their activity changes under various salt stresses were investigated, from which glycerol metabolic flow direction in the glycerol metabolic pathway was estimated. Results showed that the salinity changes had different effects on the enzymes activities. NaCl could stimulate the activities of all the four enzymes in various degrees when D. salina was grown under continuous salt stress. When treated by hyperosmotic or hypoosmotic shock, only the activity of G3pdh in D. salina was significantly stimulated. It was speculated that, under osmotic stresses, the emergency response of the cycle pathway in D. salina was driven by G3pdh via its response to the osmotic stress. Subsequently, with the changes of salinity, other three enzymes started to respond to osmotic stress. Dhar played a role of balancing the cycle metabolic pathway by its forward and backward reactions. Through synergy, the four enzymes worked together for the effective flow of the cycle metabolic pathways to maintain the glycerol requirements of cells in order to adapt to osmotic stress environments.
关键词[WOS]: GLYCEROL-3-PHOSPHATE DEHYDROGENASE ; KLEBSIELLA-PNEUMONIAE ; BETA-CAROTENE ; PURIFICATION ; FERMENTATION ; TERTIOLECTA ; REDUCTASE ; CLONING
语种: 英语
WOS记录号: WOS:000305341700017
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17039
Appears in Collections:藻类生物学及应用研究中心_期刊论文

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作者单位: 1.S China Univ Technol, Coll Food Bioengn, Guangzhou, Guangdong, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China
3.S China Univ Technol, Sch Biol Sci & Engn, Guangzhou, Guangdong, Peoples R China

Recommended Citation:
Chen, Hui; Lu, Yan; Jiang, Jian-Guo.Comparative Analysis on the Key Enzymes of the Glycerol Cycle Metabolic Pathway in Dunaliella salina under Osmotic Stresses,PLOS ONE,2012,7(6):e37578
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