IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
A Chitinase from Aeromonas veronii CD3 with the Potential to Control Myxozoan Disease
Liu, Yuchun; Zhou, Zhigang; Miao, Wei; Zhang, Yuting; Cao, Yanan; He, Suxu; Bai, Dongqing; Yao, Bin; Liu, YC (reprint author), Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100193, Peoples R China.
2011
Source PublicationPLOS ONE
ISSN1932-6203
Volume6Issue:12Pages:e29091
AbstractBackground: The class Myxosporea encompasses about 2,400 species, most of which are parasites of fish and cause serious damage in aquaculture. Due to the concerns about food safety issues and limited knowledge of Myxozoa life cycle and fish immune system, no chemicals, antibiotics or immune modulators are available to control myxozoa infection. Therefore, little can be done once Myxozoa establishment has occurred. Methodology/Principal Findings: In this paper we isolated Aeromonas veronii CD3 with significant myxospore shell valve-degrading ability from pond sediment. A 3,057-bp full-length chitinase gene was consequently cloned, and the corresponding mature, recombinant chitinase (ChiCD3) produced by Escherichia coli had substantial chitinase activity. The deduced sequence of ChiCD3 contained one catalytic domain, two chitin-binding domains, and one putative signal peptide. ChiCD3 had an optimal activity at 50u degrees C and pH 6.0, and retained more than 50% of its optimal activity under warm water aquaculture conditions (similar to 30 degrees C and pH similar to 7.0). After incubation with ChiCD3, 38.0 +/- 4.8% of the myxospores had damaged shell valves, whereas myxospores incubated with commercially available chitinases remained intact. Conclusion/Significance: This study reveals a new strategy to control myxozoan disease. ChiCD3 that has capacity to damage the shell valve of myxospores can be supplemented into fish feed and used to control Myxozoa-induced diseases specifically.
KeywordMyxobolus-cerebralis Developmental-stages Antifungal Activity Whirling Disease Myxosporea Infection Purification Resistance Bacterium Parasites
Department[Liu, Yuchun; Zhou, Zhigang; Zhang, Yuting; Cao, Yanan; He, Suxu; Yao, Bin] Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100193, Peoples R China; [Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China; [Bai, Dongqing] Tianjin Agr Univ, Fisheries Sci Dept, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin, Peoples R China
Funding OrganizationSpecial funds for Technology Development and Research for Research Institutes; Chinese Academy of Agricultural Sciences ; Special funds for Technology Development and Research for Research Institutes; Chinese Academy of Agricultural Sciences ; Special funds for Technology Development and Research for Research Institutes; Chinese Academy of Agricultural Sciences ; Special funds for Technology Development and Research for Research Institutes; Chinese Academy of Agricultural Sciences
Indexed BySCI
Language英语
WOS IDWOS:000298665600028
Funding OrganizationSpecial funds for Technology Development and Research for Research Institutes; Chinese Academy of Agricultural Sciences ; Special funds for Technology Development and Research for Research Institutes; Chinese Academy of Agricultural Sciences ; Special funds for Technology Development and Research for Research Institutes; Chinese Academy of Agricultural Sciences ; Special funds for Technology Development and Research for Research Institutes; Chinese Academy of Agricultural Sciences
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Cited Times:6[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17027
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorLiu, YC (reprint author), Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100193, Peoples R China.
Recommended Citation
GB/T 7714
Liu, Yuchun,Zhou, Zhigang,Miao, Wei,et al. A Chitinase from Aeromonas veronii CD3 with the Potential to Control Myxozoan Disease[J]. PLOS ONE,2011,6(12):e29091.
APA Liu, Yuchun.,Zhou, Zhigang.,Miao, Wei.,Zhang, Yuting.,Cao, Yanan.,...&Liu, YC .(2011).A Chitinase from Aeromonas veronii CD3 with the Potential to Control Myxozoan Disease.PLOS ONE,6(12),e29091.
MLA Liu, Yuchun,et al."A Chitinase from Aeromonas veronii CD3 with the Potential to Control Myxozoan Disease".PLOS ONE 6.12(2011):e29091.
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