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题名: A Chitinase from Aeromonas veronii CD3 with the Potential to Control Myxozoan Disease
作者: Liu, Yuchun ; Zhou, Zhigang ; Miao, Wei ; Zhang, Yuting ; Cao, Yanan ; He, Suxu ; Bai, Dongqing ; Yao, Bin
通讯作者: Liu, YC (reprint author), Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100193, Peoples R China.
关键词: MYXOBOLUS-CEREBRALIS ; DEVELOPMENTAL-STAGES ; ANTIFUNGAL ACTIVITY ; WHIRLING DISEASE ; MYXOSPOREA ; INFECTION ; PURIFICATION ; RESISTANCE ; BACTERIUM ; PARASITES
刊名: PLOS ONE
发表日期: 2011
卷: 6, 期:12, 页:e29091
收录类别: SCI
部门归属: [Liu, Yuchun; Zhou, Zhigang; Zhang, Yuting; Cao, Yanan; He, Suxu; Yao, Bin] Chinese Acad Agr Sci, Feed Res Inst, Minist Agr, Key Lab Feed Biotechnol, Beijing 100193, Peoples R China; [Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China; [Bai, Dongqing] Tianjin Agr Univ, Fisheries Sci Dept, Tianjin Key Lab Aquaecol & Aquaculture, Tianjin, Peoples R China
资助者: Special funds for Technology Development and Research for Research Institutes; Chinese Academy of Agricultural Sciences
摘要: Background: The class Myxosporea encompasses about 2,400 species, most of which are parasites of fish and cause serious damage in aquaculture. Due to the concerns about food safety issues and limited knowledge of Myxozoa life cycle and fish immune system, no chemicals, antibiotics or immune modulators are available to control myxozoa infection. Therefore, little can be done once Myxozoa establishment has occurred. Methodology/Principal Findings: In this paper we isolated Aeromonas veronii CD3 with significant myxospore shell valve-degrading ability from pond sediment. A 3,057-bp full-length chitinase gene was consequently cloned, and the corresponding mature, recombinant chitinase (ChiCD3) produced by Escherichia coli had substantial chitinase activity. The deduced sequence of ChiCD3 contained one catalytic domain, two chitin-binding domains, and one putative signal peptide. ChiCD3 had an optimal activity at 50u degrees C and pH 6.0, and retained more than 50% of its optimal activity under warm water aquaculture conditions (similar to 30 degrees C and pH similar to 7.0). After incubation with ChiCD3, 38.0 +/- 4.8% of the myxospores had damaged shell valves, whereas myxospores incubated with commercially available chitinases remained intact. Conclusion/Significance: This study reveals a new strategy to control myxozoan disease. ChiCD3 that has capacity to damage the shell valve of myxospores can be supplemented into fish feed and used to control Myxozoa-induced diseases specifically.
语种: 英语
WOS记录号: WOS:000298665600028
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17027
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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Recommended Citation:
Liu, Yuchun; Zhou, Zhigang; Miao, Wei; Zhang, Yuting; Cao, Yanan; He, Suxu; Bai, Dongqing; Yao, Bin.A Chitinase from Aeromonas veronii CD3 with the Potential to Control Myxozoan Disease,PLOS ONE,2011,6(12):e29091
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