IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Zebrafish Mms2 promotes K63-linked polyubiquitination and is involved in p53-mediated DNA-damage response
Wen, Rui2,3; Li, Jie1; Xu, Xin2; Cui, Zongbin1; Xiao, Wei2,3; Cui, ZB (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
2012-02-01
Source PublicationDNA REPAIR
ISSN1568-7864
Volume11Issue:2Pages:157-166
AbstractThe ubiquitin-conjugating enzyme Ubc13 together with a Ubc/E2 variant (Uev) form a stable complex and mediate K63-linked polyubiquitination, which is implicated in DNA damage tolerance in yeast and mammalian cells. The zebrafish Danio rerio is a lower vertebrate model organism widely used in the studies of vertebrate development and environmental stress responses. Here we report the identification and functional characterization of two zebrafish UEV genes, Drmms2 and Druev1. Their deduced amino acid sequences indicate that the two UEV genes evolved separately prior to the appearance of vertebrates. Both zebrafish Uevs form a stable complex with DrUbc13 as well as Ubc13s from yeast and human, and are able to promote Ubc13-mediated K63 polyubiquitination in vitro, suggesting that their biochemical activities are conserved. Despite the fact that both zebrafish UEV genes can functionally replace the yeast MMS2 DNA-damage tolerance function, they exhibited differences in DNA-damage response in zebrafish embryos: ablation of DrMms2, but not DrUev1, enhances both spontaneous and DNA-damage induced expression of p53 effectors p21 and mdm2. In addition, DrUbc13 specifically binds Drp53 in an in vitro assay. These observations collectively indicate that zebrafish Mms2 and Ubc13 form a stable complex, which is required for p53-mediated DNA-damage response. (C) 2011 Elsevier B.V. All rights reserved.; The ubiquitin-conjugating enzyme Ubc13 together with a Ubc/E2 variant (Uev) form a stable complex and mediate K63-linked polyubiquitination, which is implicated in DNA damage tolerance in yeast and mammalian cells. The zebrafish Danio rerio is a lower vertebrate model organism widely used in the studies of vertebrate development and environmental stress responses. Here we report the identification and functional characterization of two zebrafish UEV genes, Drmms2 and Druev1. Their deduced amino acid sequences indicate that the two UEV genes evolved separately prior to the appearance of vertebrates. Both zebrafish Uevs form a stable complex with DrUbc13 as well as Ubc13s from yeast and human, and are able to promote Ubc13-mediated K63 polyubiquitination in vitro, suggesting that their biochemical activities are conserved. Despite the fact that both zebrafish UEV genes can functionally replace the yeast MMS2 DNA-damage tolerance function, they exhibited differences in DNA-damage response in zebrafish embryos: ablation of DrMms2, but not DrUev1, enhances both spontaneous and DNA-damage induced expression of p53 effectors p21 and mdm2. In addition, DrUbc13 specifically binds Drp53 in an in vitro assay. These observations collectively indicate that zebrafish Mms2 and Ubc13 form a stable complex, which is required for p53-mediated DNA-damage response. (C) 2011 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordZebrafish Ubc13 Mms2 K63-linked Polyubiquitination Dna-damage Tolerance P53
Department[Li, Jie; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China; [Wen, Rui; Xu, Xin; Xiao, Wei] Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China; [Wen, Rui; Xiao, Wei] Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
DOI10.1016/j.dnarep.2011.10.015
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationCapital Normal University[10531182313]; Canadian Institutes of Health Research[MOP-93612]; National Natural Science Foundation of China[30871442, 31171390] ; Capital Normal University[10531182313]; Canadian Institutes of Health Research[MOP-93612]; National Natural Science Foundation of China[30871442, 31171390] ; Capital Normal University[10531182313]; Canadian Institutes of Health Research[MOP-93612]; National Natural Science Foundation of China[30871442, 31171390] ; Capital Normal University[10531182313]; Canadian Institutes of Health Research[MOP-93612]; National Natural Science Foundation of China[30871442, 31171390]
Indexed BySCI
Language英语
WOS Research AreaGenetics & Heredity ; Toxicology
WOS SubjectGenetics & Heredity ; Toxicology
WOS IDWOS:000301618900008
WOS KeywordUBIQUITIN-CONJUGATING-ENZYME ; CELL NUCLEAR ANTIGEN ; NF-KAPPA-B ; POSTREPLICATION REPAIR PATHWAY ; PROTEIN-PROTEIN INTERACTIONS ; SACCHAROMYCES-CEREVISIAE ; IN-VITRO ; SCHIZOSACCHAROMYCES-POMBE ; GENOMIC INSTABILITY ; DEPENDENT KINASE
Funding OrganizationCapital Normal University[10531182313]; Canadian Institutes of Health Research[MOP-93612]; National Natural Science Foundation of China[30871442, 31171390] ; Capital Normal University[10531182313]; Canadian Institutes of Health Research[MOP-93612]; National Natural Science Foundation of China[30871442, 31171390] ; Capital Normal University[10531182313]; Canadian Institutes of Health Research[MOP-93612]; National Natural Science Foundation of China[30871442, 31171390] ; Capital Normal University[10531182313]; Canadian Institutes of Health Research[MOP-93612]; National Natural Science Foundation of China[30871442, 31171390]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17023
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorCui, ZB (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.Capital Normal Univ, Coll Life Sci, Beijing 100048, Peoples R China
3.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada
Recommended Citation
GB/T 7714
Wen, Rui,Li, Jie,Xu, Xin,et al. Zebrafish Mms2 promotes K63-linked polyubiquitination and is involved in p53-mediated DNA-damage response[J]. DNA REPAIR,2012,11(2):157-166.
APA Wen, Rui,Li, Jie,Xu, Xin,Cui, Zongbin,Xiao, Wei,&Cui, ZB .(2012).Zebrafish Mms2 promotes K63-linked polyubiquitination and is involved in p53-mediated DNA-damage response.DNA REPAIR,11(2),157-166.
MLA Wen, Rui,et al."Zebrafish Mms2 promotes K63-linked polyubiquitination and is involved in p53-mediated DNA-damage response".DNA REPAIR 11.2(2012):157-166.
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