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Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish
Lou, Qiyong2; He, Jiangyan; Hu, Liang; Yin, Zhan1; Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China.
2012-05-01
Source PublicationBIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
ISSN0167-4889
Volume1823Issue:5Pages:1024-1032
AbstractIt has been suggested that mouse lbx1 is essential for directing hypaxial myogenic precursor cell migration. In zebrafish, the expression of lbx1a, lbx1b, and lbx2 has been observed in pectoral fin buds. It has also been shown that knocking down endogenous lbx2 in zebrafish embryos diminishes myoD expression in the pectoral fin bud. However, downstream lbxs signals remain largely unexplored. Here, we describe a previously unknown function of zebrafish lbx2 (lbx2) during convergent extension (CE) movements. The abrogation of the lbx2 function by two non-overlapping morpholino oligonucleotides (MOs) resulted in the defective convergence and extension movements in morphants during gastrulation. Our transplantation studies further demonstrated that the overexpression of lbx2 autonomously promotes CE movements. Expression of wnt5b is significantly reduced in lbx2 morphants. We have demonstrated that application of the wnt5b MO, a dominant-negative form of disheveled (DvI) and a chemical inhibitor of Rho-associated kinase Y27632 in zebrafish embryos have effects reminiscent that are of the CE and hypaxial myogenesis defects observed in lbx2 morphants. Moreover, the CE and hypaxial mesoderm defects seen in lbx2 morphants can be rescued by co-injection with wnt5b or RhoA mRNA. However, this reduced level of active RhoA and hypaxial myogenesis defects in the embryos injected with the dominant-negative form of Dvl mRNA cannot be effectively restored by co-injection with lbx2 mRNA. Our results suggest that the key noncanonical Wnt signaling components WntS, Dvl, and RhoA are downstream effectors involved in the regulative roles of lbx2 in CE movement and hypaxial myogenesis during zebrafish embryogenesis. (C) 2012 Elsevier B.V. All rights reserved.; It has been suggested that mouse lbx1 is essential for directing hypaxial myogenic precursor cell migration. In zebrafish, the expression of lbx1a, lbx1b, and lbx2 has been observed in pectoral fin buds. It has also been shown that knocking down endogenous lbx2 in zebrafish embryos diminishes myoD expression in the pectoral fin bud. However, downstream lbxs signals remain largely unexplored. Here, we describe a previously unknown function of zebrafish lbx2 (lbx2) during convergent extension (CE) movements. The abrogation of the lbx2 function by two non-overlapping morpholino oligonucleotides (MOs) resulted in the defective convergence and extension movements in morphants during gastrulation. Our transplantation studies further demonstrated that the overexpression of lbx2 autonomously promotes CE movements. Expression of wnt5b is significantly reduced in lbx2 morphants. We have demonstrated that application of the wnt5b MO, a dominant-negative form of disheveled (DvI) and a chemical inhibitor of Rho-associated kinase Y27632 in zebrafish embryos have effects reminiscent that are of the CE and hypaxial myogenesis defects observed in lbx2 morphants. Moreover, the CE and hypaxial mesoderm defects seen in lbx2 morphants can be rescued by co-injection with wnt5b or RhoA mRNA. However, this reduced level of active RhoA and hypaxial myogenesis defects in the embryos injected with the dominant-negative form of Dvl mRNA cannot be effectively restored by co-injection with lbx2 mRNA. Our results suggest that the key noncanonical Wnt signaling components WntS, Dvl, and RhoA are downstream effectors involved in the regulative roles of lbx2 in CE movement and hypaxial myogenesis during zebrafish embryogenesis. (C) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordZebrafish Lbx2 Noncanonical Wnt Gastrulation
Department[Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China; [Lou, Qiyong] Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
DOI10.1016/j.bbamcr.2012.02.013
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Cell Biology
WOS SubjectBiochemistry & Molecular Biology ; Cell Biology
WOS IDWOS:000303618000006
WOS KeywordRHO GTPASES ; WILD-TYPE ; NO TAIL ; GASTRULATION ; EXPRESSION ; EMBRYO ; MUSCLE ; MAINTENANCE ; PRECURSORS ; REGULATOR
Funding OrganizationNational Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402]
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Cited Times:8[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17019
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
Recommended Citation
GB/T 7714
Lou, Qiyong,He, Jiangyan,Hu, Liang,et al. Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2012,1823(5):1024-1032.
APA Lou, Qiyong,He, Jiangyan,Hu, Liang,Yin, Zhan,&Yin, Z .(2012).Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,1823(5),1024-1032.
MLA Lou, Qiyong,et al."Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1823.5(2012):1024-1032.
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