| Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish | |
| Lou, Qiyong2; He, Jiangyan; Hu, Liang; Yin, Zhan1; Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China. | |
| 2012-05-01 | |
| Source Publication | BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
 |
| ISSN | 0167-4889 |
| Volume | 1823Issue:5Pages:1024-1032 |
| Abstract | It has been suggested that mouse lbx1 is essential for directing hypaxial myogenic precursor cell migration. In zebrafish, the expression of lbx1a, lbx1b, and lbx2 has been observed in pectoral fin buds. It has also been shown that knocking down endogenous lbx2 in zebrafish embryos diminishes myoD expression in the pectoral fin bud. However, downstream lbxs signals remain largely unexplored. Here, we describe a previously unknown function of zebrafish lbx2 (lbx2) during convergent extension (CE) movements. The abrogation of the lbx2 function by two non-overlapping morpholino oligonucleotides (MOs) resulted in the defective convergence and extension movements in morphants during gastrulation. Our transplantation studies further demonstrated that the overexpression of lbx2 autonomously promotes CE movements. Expression of wnt5b is significantly reduced in lbx2 morphants. We have demonstrated that application of the wnt5b MO, a dominant-negative form of disheveled (DvI) and a chemical inhibitor of Rho-associated kinase Y27632 in zebrafish embryos have effects reminiscent that are of the CE and hypaxial myogenesis defects observed in lbx2 morphants. Moreover, the CE and hypaxial mesoderm defects seen in lbx2 morphants can be rescued by co-injection with wnt5b or RhoA mRNA. However, this reduced level of active RhoA and hypaxial myogenesis defects in the embryos injected with the dominant-negative form of Dvl mRNA cannot be effectively restored by co-injection with lbx2 mRNA. Our results suggest that the key noncanonical Wnt signaling components WntS, Dvl, and RhoA are downstream effectors involved in the regulative roles of lbx2 in CE movement and hypaxial myogenesis during zebrafish embryogenesis. (C) 2012 Elsevier B.V. All rights reserved.; It has been suggested that mouse lbx1 is essential for directing hypaxial myogenic precursor cell migration. In zebrafish, the expression of lbx1a, lbx1b, and lbx2 has been observed in pectoral fin buds. It has also been shown that knocking down endogenous lbx2 in zebrafish embryos diminishes myoD expression in the pectoral fin bud. However, downstream lbxs signals remain largely unexplored. Here, we describe a previously unknown function of zebrafish lbx2 (lbx2) during convergent extension (CE) movements. The abrogation of the lbx2 function by two non-overlapping morpholino oligonucleotides (MOs) resulted in the defective convergence and extension movements in morphants during gastrulation. Our transplantation studies further demonstrated that the overexpression of lbx2 autonomously promotes CE movements. Expression of wnt5b is significantly reduced in lbx2 morphants. We have demonstrated that application of the wnt5b MO, a dominant-negative form of disheveled (DvI) and a chemical inhibitor of Rho-associated kinase Y27632 in zebrafish embryos have effects reminiscent that are of the CE and hypaxial myogenesis defects observed in lbx2 morphants. Moreover, the CE and hypaxial mesoderm defects seen in lbx2 morphants can be rescued by co-injection with wnt5b or RhoA mRNA. However, this reduced level of active RhoA and hypaxial myogenesis defects in the embryos injected with the dominant-negative form of Dvl mRNA cannot be effectively restored by co-injection with lbx2 mRNA. Our results suggest that the key noncanonical Wnt signaling components WntS, Dvl, and RhoA are downstream effectors involved in the regulative roles of lbx2 in CE movement and hypaxial myogenesis during zebrafish embryogenesis. (C) 2012 Elsevier B.V. All rights reserved. |
| Subtype | Article |
| Keyword | Zebrafish Lbx2 Noncanonical Wnt Gastrulation |
| Department | [Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China; [Lou, Qiyong] Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| DOI | 10.1016/j.bbamcr.2012.02.013 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Biochemistry & Molecular Biology ; Cell Biology |
| WOS Subject | Biochemistry & Molecular Biology ; Cell Biology |
| WOS ID | WOS:000303618000006 |
| WOS Keyword | RHO GTPASES ; WILD-TYPE ; NO TAIL ; GASTRULATION ; EXPRESSION ; EMBRYO ; MUSCLE ; MAINTENANCE ; PRECURSORS ; REGULATOR |
| Funding Organization | National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] ; National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/17019 |
| Collection | 水生生物分子与细胞生物学研究中心_期刊论文 |
| Corresponding Author | Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
| Recommended Citation GB/T 7714 | Lou, Qiyong,He, Jiangyan,Hu, Liang,et al. Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2012,1823(5):1024-1032. |
| APA | Lou, Qiyong,He, Jiangyan,Hu, Liang,Yin, Zhan,&Yin, Z .(2012).Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,1823(5),1024-1032. |
| MLA | Lou, Qiyong,et al."Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH 1823.5(2012):1024-1032. |
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