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题名: Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish
作者: Lou, Qiyong2; He, Jiangyan; Hu, Liang; Yin, Zhan1
通讯作者: Yin, Z (reprint author), Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, 7 Donghu S Rd, Wuhan 430072, Hubei, Peoples R China.
关键词: Zebrafish ; lbx2 ; Noncanonical Wnt ; Gastrulation
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
发表日期: 2012-05-01
DOI: 10.1016/j.bbamcr.2012.02.013
卷: 1823, 期:5, 页:1024-1032
收录类别: SCI
文章类型: Article
部门归属: [Yin, Zhan] Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China; [Lou, Qiyong] Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Basic Research Program of China (973 Program)[2010CB126302]; National Natural Science Foundation of China[30925027, 30871402]
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
摘要: It has been suggested that mouse lbx1 is essential for directing hypaxial myogenic precursor cell migration. In zebrafish, the expression of lbx1a, lbx1b, and lbx2 has been observed in pectoral fin buds. It has also been shown that knocking down endogenous lbx2 in zebrafish embryos diminishes myoD expression in the pectoral fin bud. However, downstream lbxs signals remain largely unexplored. Here, we describe a previously unknown function of zebrafish lbx2 (lbx2) during convergent extension (CE) movements. The abrogation of the lbx2 function by two non-overlapping morpholino oligonucleotides (MOs) resulted in the defective convergence and extension movements in morphants during gastrulation. Our transplantation studies further demonstrated that the overexpression of lbx2 autonomously promotes CE movements. Expression of wnt5b is significantly reduced in lbx2 morphants. We have demonstrated that application of the wnt5b MO, a dominant-negative form of disheveled (DvI) and a chemical inhibitor of Rho-associated kinase Y27632 in zebrafish embryos have effects reminiscent that are of the CE and hypaxial myogenesis defects observed in lbx2 morphants. Moreover, the CE and hypaxial mesoderm defects seen in lbx2 morphants can be rescued by co-injection with wnt5b or RhoA mRNA. However, this reduced level of active RhoA and hypaxial myogenesis defects in the embryos injected with the dominant-negative form of Dvl mRNA cannot be effectively restored by co-injection with lbx2 mRNA. Our results suggest that the key noncanonical Wnt signaling components WntS, Dvl, and RhoA are downstream effectors involved in the regulative roles of lbx2 in CE movement and hypaxial myogenesis during zebrafish embryogenesis. (C) 2012 Elsevier B.V. All rights reserved.
英文摘要: It has been suggested that mouse lbx1 is essential for directing hypaxial myogenic precursor cell migration. In zebrafish, the expression of lbx1a, lbx1b, and lbx2 has been observed in pectoral fin buds. It has also been shown that knocking down endogenous lbx2 in zebrafish embryos diminishes myoD expression in the pectoral fin bud. However, downstream lbxs signals remain largely unexplored. Here, we describe a previously unknown function of zebrafish lbx2 (lbx2) during convergent extension (CE) movements. The abrogation of the lbx2 function by two non-overlapping morpholino oligonucleotides (MOs) resulted in the defective convergence and extension movements in morphants during gastrulation. Our transplantation studies further demonstrated that the overexpression of lbx2 autonomously promotes CE movements. Expression of wnt5b is significantly reduced in lbx2 morphants. We have demonstrated that application of the wnt5b MO, a dominant-negative form of disheveled (DvI) and a chemical inhibitor of Rho-associated kinase Y27632 in zebrafish embryos have effects reminiscent that are of the CE and hypaxial myogenesis defects observed in lbx2 morphants. Moreover, the CE and hypaxial mesoderm defects seen in lbx2 morphants can be rescued by co-injection with wnt5b or RhoA mRNA. However, this reduced level of active RhoA and hypaxial myogenesis defects in the embryos injected with the dominant-negative form of Dvl mRNA cannot be effectively restored by co-injection with lbx2 mRNA. Our results suggest that the key noncanonical Wnt signaling components WntS, Dvl, and RhoA are downstream effectors involved in the regulative roles of lbx2 in CE movement and hypaxial myogenesis during zebrafish embryogenesis. (C) 2012 Elsevier B.V. All rights reserved.
关键词[WOS]: RHO GTPASES ; WILD-TYPE ; NO TAIL ; GASTRULATION ; EXPRESSION ; EMBRYO ; MUSCLE ; MAINTENANCE ; PRECURSORS ; REGULATOR
语种: 英语
WOS记录号: WOS:000303618000006
ISSN号: 0167-4889
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17019
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Ctr Water Environm & Human Hlth, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China

Recommended Citation:
Lou, Qiyong; He, Jiangyan; Hu, Liang; Yin, Zhan.Role of lbx2 in the noncanonical Wnt signaling pathway for convergence and extension movements and hypaxial myogenesis in zebrafish,BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,2012,1823(5):1024-1032
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