IHB OpenIR  > 水生生物分子与细胞生物学研究中心  > 期刊论文
Transcriptomic Characterization of Temperature Stress Responses in Larval Zebrafish
Long, Yong1; Li, Linchun2; Li, Qing1; He, Xiaozhen1; Cui, Zongbin1; Long, Y (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
2012-05-30
Source PublicationPLOS ONE
ISSN1932-6203
Volume7Issue:5Pages:e37209
AbstractTemperature influences nearly all biochemical, physiological and life history activities of fish, but the molecular mechanisms underlying the temperature acclimation remains largely unknown. Previous studies have identified many temperature-regulated genes in adult tissues; however, the transcriptional responses of fish larvae to temperature stress are not well understood. In this study, we characterized the transcriptional responses in larval zebrafish exposed to cold or heat stress using microarray analysis. In comparison with genes expressed in the control at 28 degrees C, a total of 2680 genes were found to be affected in 96 hpf larvae exposed to cold (16 degrees C) or heat (34 degrees C) for 2 and 48 h and most of these genes were expressed in a temperature-specific and temporally regulated manner. Bioinformatic analysis identified multiple temperature-regulated biological processes and pathways. Biological processes overrepresented among the earliest genes induced by temperature stress include regulation of transcription, nucleosome assembly, chromatin organization and protein folding. However, processes such as RNA processing, cellular metal ion homeostasis and protein transport and were enriched in genes up-regulated under cold exposure for 48 h. Pathways such as mTOR signalling, p53 signalling and circadian rhythm were enriched among cold-induced genes, while adipocytokine signalling, protein export and arginine and praline metabolism were enriched among heat-induced genes. Although most of these biological processes and pathways were specifically regulated by cold or heat, common responses to both cold and heat stresses were also found. Thus, these findings provide new interesting clues for elucidation of mechanisms underlying the temperature acclimation in fish.; Temperature influences nearly all biochemical, physiological and life history activities of fish, but the molecular mechanisms underlying the temperature acclimation remains largely unknown. Previous studies have identified many temperature-regulated genes in adult tissues; however, the transcriptional responses of fish larvae to temperature stress are not well understood. In this study, we characterized the transcriptional responses in larval zebrafish exposed to cold or heat stress using microarray analysis. In comparison with genes expressed in the control at 28 degrees C, a total of 2680 genes were found to be affected in 96 hpf larvae exposed to cold (16 degrees C) or heat (34 degrees C) for 2 and 48 h and most of these genes were expressed in a temperature-specific and temporally regulated manner. Bioinformatic analysis identified multiple temperature-regulated biological processes and pathways. Biological processes overrepresented among the earliest genes induced by temperature stress include regulation of transcription, nucleosome assembly, chromatin organization and protein folding. However, processes such as RNA processing, cellular metal ion homeostasis and protein transport and were enriched in genes up-regulated under cold exposure for 48 h. Pathways such as mTOR signalling, p53 signalling and circadian rhythm were enriched among cold-induced genes, while adipocytokine signalling, protein export and arginine and praline metabolism were enriched among heat-induced genes. Although most of these biological processes and pathways were specifically regulated by cold or heat, common responses to both cold and heat stresses were also found. Thus, these findings provide new interesting clues for elucidation of mechanisms underlying the temperature acclimation in fish.
SubtypeArticle
KeywordCoral-reef Fish Nuclear Hormone-receptors Rev-erb-alpha Gene-expression Cold-acclimation Heat-stress Gillichthys-mirabilis Thermal-acclimation Microarray Fos
Department[Long, Yong; Li, Qing; He, Xiaozhen; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; [Li, Linchun] Xiamen Ocean Vocat Coll, Dept Biotechnol, Xiamen, Fujian, Peoples R China
DOI10.1371/journal.pone.0037209
WOS HeadingsScience & Technology
Funding OrganizationNational Natural Science Foundation of China[31101892]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[31101892]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[31101892]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[31101892]; National High-tech RD (863) Program[2007AA10Z164]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000305353400021
WOS KeywordCORAL-REEF FISH ; NUCLEAR HORMONE-RECEPTORS ; REV-ERB-ALPHA ; GENE-EXPRESSION ; COLD-ACCLIMATION ; HEAT-STRESS ; GILLICHTHYS-MIRABILIS ; THERMAL-ACCLIMATION ; MICROARRAY ; FOS
Funding OrganizationNational Natural Science Foundation of China[31101892]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[31101892]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[31101892]; National High-tech RD (863) Program[2007AA10Z164] ; National Natural Science Foundation of China[31101892]; National High-tech RD (863) Program[2007AA10Z164]
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Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17017
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorLong, Y (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
2.Xiamen Ocean Vocat Coll, Dept Biotechnol, Xiamen, Fujian, Peoples R China
Recommended Citation
GB/T 7714
Long, Yong,Li, Linchun,Li, Qing,et al. Transcriptomic Characterization of Temperature Stress Responses in Larval Zebrafish[J]. PLOS ONE,2012,7(5):e37209.
APA Long, Yong,Li, Linchun,Li, Qing,He, Xiaozhen,Cui, Zongbin,&Long, Y .(2012).Transcriptomic Characterization of Temperature Stress Responses in Larval Zebrafish.PLOS ONE,7(5),e37209.
MLA Long, Yong,et al."Transcriptomic Characterization of Temperature Stress Responses in Larval Zebrafish".PLOS ONE 7.5(2012):e37209.
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