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题名: Transcriptomic Characterization of Temperature Stress Responses in Larval Zebrafish
作者: Long, Yong1; Li, Linchun2; Li, Qing1; He, Xiaozhen1; Cui, Zongbin1
通讯作者: Long, Y (reprint author), Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China.
关键词: CORAL-REEF FISH ; NUCLEAR HORMONE-RECEPTORS ; REV-ERB-ALPHA ; GENE-EXPRESSION ; COLD-ACCLIMATION ; HEAT-STRESS ; GILLICHTHYS-MIRABILIS ; THERMAL-ACCLIMATION ; MICROARRAY ; FOS
刊名: PLOS ONE
发表日期: 2012-05-30
DOI: 10.1371/journal.pone.0037209
卷: 7, 期:5, 页:e37209
收录类别: SCI
文章类型: Article
部门归属: [Long, Yong; Li, Qing; He, Xiaozhen; Cui, Zongbin] Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China; [Li, Linchun] Xiamen Ocean Vocat Coll, Dept Biotechnol, Xiamen, Fujian, Peoples R China
WOS标题词: Science & Technology
资助者: National Natural Science Foundation of China[31101892]; National High-tech RD (863) Program[2007AA10Z164]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Temperature influences nearly all biochemical, physiological and life history activities of fish, but the molecular mechanisms underlying the temperature acclimation remains largely unknown. Previous studies have identified many temperature-regulated genes in adult tissues; however, the transcriptional responses of fish larvae to temperature stress are not well understood. In this study, we characterized the transcriptional responses in larval zebrafish exposed to cold or heat stress using microarray analysis. In comparison with genes expressed in the control at 28 degrees C, a total of 2680 genes were found to be affected in 96 hpf larvae exposed to cold (16 degrees C) or heat (34 degrees C) for 2 and 48 h and most of these genes were expressed in a temperature-specific and temporally regulated manner. Bioinformatic analysis identified multiple temperature-regulated biological processes and pathways. Biological processes overrepresented among the earliest genes induced by temperature stress include regulation of transcription, nucleosome assembly, chromatin organization and protein folding. However, processes such as RNA processing, cellular metal ion homeostasis and protein transport and were enriched in genes up-regulated under cold exposure for 48 h. Pathways such as mTOR signalling, p53 signalling and circadian rhythm were enriched among cold-induced genes, while adipocytokine signalling, protein export and arginine and praline metabolism were enriched among heat-induced genes. Although most of these biological processes and pathways were specifically regulated by cold or heat, common responses to both cold and heat stresses were also found. Thus, these findings provide new interesting clues for elucidation of mechanisms underlying the temperature acclimation in fish.
英文摘要: Temperature influences nearly all biochemical, physiological and life history activities of fish, but the molecular mechanisms underlying the temperature acclimation remains largely unknown. Previous studies have identified many temperature-regulated genes in adult tissues; however, the transcriptional responses of fish larvae to temperature stress are not well understood. In this study, we characterized the transcriptional responses in larval zebrafish exposed to cold or heat stress using microarray analysis. In comparison with genes expressed in the control at 28 degrees C, a total of 2680 genes were found to be affected in 96 hpf larvae exposed to cold (16 degrees C) or heat (34 degrees C) for 2 and 48 h and most of these genes were expressed in a temperature-specific and temporally regulated manner. Bioinformatic analysis identified multiple temperature-regulated biological processes and pathways. Biological processes overrepresented among the earliest genes induced by temperature stress include regulation of transcription, nucleosome assembly, chromatin organization and protein folding. However, processes such as RNA processing, cellular metal ion homeostasis and protein transport and were enriched in genes up-regulated under cold exposure for 48 h. Pathways such as mTOR signalling, p53 signalling and circadian rhythm were enriched among cold-induced genes, while adipocytokine signalling, protein export and arginine and praline metabolism were enriched among heat-induced genes. Although most of these biological processes and pathways were specifically regulated by cold or heat, common responses to both cold and heat stresses were also found. Thus, these findings provide new interesting clues for elucidation of mechanisms underlying the temperature acclimation in fish.
关键词[WOS]: CORAL-REEF FISH ; NUCLEAR HORMONE-RECEPTORS ; REV-ERB-ALPHA ; GENE-EXPRESSION ; COLD-ACCLIMATION ; HEAT-STRESS ; GILLICHTHYS-MIRABILIS ; THERMAL-ACCLIMATION ; MICROARRAY ; FOS
语种: 英语
WOS记录号: WOS:000305353400021
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17017
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan, Hubei, Peoples R China
2.Xiamen Ocean Vocat Coll, Dept Biotechnol, Xiamen, Fujian, Peoples R China

Recommended Citation:
Long, Yong; Li, Linchun; Li, Qing; He, Xiaozhen; Cui, Zongbin.Transcriptomic Characterization of Temperature Stress Responses in Larval Zebrafish,PLOS ONE,2012,7(5):e37209
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