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Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals
Ji, Cheng1,2; Jin, Xia1; He, Jiangyan1; Yin, Zhan1; Yin, Z (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China.
2012-07-15
Source PublicationTOXICOLOGY AND APPLIED PHARMACOLOGY
ISSN0041-008X
Volume262Issue:2Pages:149-155
AbstractAccumulating evidence indicates that a wide range of chemicals have the ability to interfere with the hypothalamic-pituitary-thyroid (HPT) axis. Novel endpoints should be evaluated in addition to existing methods in order to effectively assess the effects of these chemicals on the HPT axis. Thyroid-stimulating hormone subunit beta (TSH beta) plays central regulatory roles in the HPT system. We identified the regulatory region that determines the expression level of zebrafish TSH beta in the anterior pituitary. In the transgenic zebrafish with EGFP driven by the TSH beta promoter, the similar responsive patterns between the expression levels of TSH beta:EGFP and endogenous TSH beta mRNA in the pituitary are observed following treatments with goitrogen chemicals and exogenous thyroid hormones (THs). These results suggest that the TSH beta:EGFP transgenic reporter zebrafish may be a useful alternative in vivo model for the assessment of chemicals interfering with the HPT system. (C) 2012 Elsevier Inc. All rights reserved.; Accumulating evidence indicates that a wide range of chemicals have the ability to interfere with the hypothalamic-pituitary-thyroid (HPT) axis. Novel endpoints should be evaluated in addition to existing methods in order to effectively assess the effects of these chemicals on the HPT axis. Thyroid-stimulating hormone subunit beta (TSH beta) plays central regulatory roles in the HPT system. We identified the regulatory region that determines the expression level of zebrafish TSH beta in the anterior pituitary. In the transgenic zebrafish with EGFP driven by the TSH beta promoter, the similar responsive patterns between the expression levels of TSH beta:EGFP and endogenous TSH beta mRNA in the pituitary are observed following treatments with goitrogen chemicals and exogenous thyroid hormones (THs). These results suggest that the TSH beta:EGFP transgenic reporter zebrafish may be a useful alternative in vivo model for the assessment of chemicals interfering with the HPT system. (C) 2012 Elsevier Inc. All rights reserved.
SubtypeArticle
KeywordThyroid-stimulating Hormone Subunit-beta Hypothalamic-pituitary-thyroid Endocrine Disrupting Transgenic Reporter Fish Assessment Model
Department[Ji, Cheng; Jin, Xia; He, Jiangyan; Yin, Zhan] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China; [Ji, Cheng] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
DOI10.1016/j.taap.2012.04.029
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationHi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012] ; Hi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012] ; Hi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012] ; Hi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012]
Indexed BySCI
Language英语
WOS Research AreaPharmacology & Pharmacy ; Toxicology
WOS SubjectPharmacology & Pharmacy ; Toxicology
WOS IDWOS:000305845400006
WOS KeywordTHYROTROPIN-BETA-SUBUNIT ; ENDOCRINE DISRUPTION ; GLAND ; GENE ; EXPRESSION ; PROMOTER ; ACTIVATION ; REGION ; BRAIN
Funding OrganizationHi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012] ; Hi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012] ; Hi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012] ; Hi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/17015
Collection水生生物分子与细胞生物学研究中心_期刊论文
Corresponding AuthorYin, Z (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China.
Affiliation1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Ji, Cheng,Jin, Xia,He, Jiangyan,et al. Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2012,262(2):149-155.
APA Ji, Cheng,Jin, Xia,He, Jiangyan,Yin, Zhan,&Yin, Z .(2012).Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals.TOXICOLOGY AND APPLIED PHARMACOLOGY,262(2),149-155.
MLA Ji, Cheng,et al."Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals".TOXICOLOGY AND APPLIED PHARMACOLOGY 262.2(2012):149-155.
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