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题名: Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals
作者: Ji, Cheng1, 2; Jin, Xia1; He, Jiangyan1; Yin, Zhan1
通讯作者: Yin, Z (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China.
关键词: Thyroid-stimulating hormone subunit-beta ; Hypothalamic-pituitary-thyroid ; Endocrine disrupting ; Transgenic reporter fish ; Assessment model
刊名: TOXICOLOGY AND APPLIED PHARMACOLOGY
发表日期: 2012-07-15
DOI: 10.1016/j.taap.2012.04.029
卷: 262, 期:2, 页:149-155
收录类别: SCI
文章类型: Article
部门归属: [Ji, Cheng; Jin, Xia; He, Jiangyan; Yin, Zhan] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China; [Ji, Cheng] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Hi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012]
类目[WOS]: Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]: Pharmacology & Pharmacy ; Toxicology
摘要: Accumulating evidence indicates that a wide range of chemicals have the ability to interfere with the hypothalamic-pituitary-thyroid (HPT) axis. Novel endpoints should be evaluated in addition to existing methods in order to effectively assess the effects of these chemicals on the HPT axis. Thyroid-stimulating hormone subunit beta (TSH beta) plays central regulatory roles in the HPT system. We identified the regulatory region that determines the expression level of zebrafish TSH beta in the anterior pituitary. In the transgenic zebrafish with EGFP driven by the TSH beta promoter, the similar responsive patterns between the expression levels of TSH beta:EGFP and endogenous TSH beta mRNA in the pituitary are observed following treatments with goitrogen chemicals and exogenous thyroid hormones (THs). These results suggest that the TSH beta:EGFP transgenic reporter zebrafish may be a useful alternative in vivo model for the assessment of chemicals interfering with the HPT system. (C) 2012 Elsevier Inc. All rights reserved.
英文摘要: Accumulating evidence indicates that a wide range of chemicals have the ability to interfere with the hypothalamic-pituitary-thyroid (HPT) axis. Novel endpoints should be evaluated in addition to existing methods in order to effectively assess the effects of these chemicals on the HPT axis. Thyroid-stimulating hormone subunit beta (TSH beta) plays central regulatory roles in the HPT system. We identified the regulatory region that determines the expression level of zebrafish TSH beta in the anterior pituitary. In the transgenic zebrafish with EGFP driven by the TSH beta promoter, the similar responsive patterns between the expression levels of TSH beta:EGFP and endogenous TSH beta mRNA in the pituitary are observed following treatments with goitrogen chemicals and exogenous thyroid hormones (THs). These results suggest that the TSH beta:EGFP transgenic reporter zebrafish may be a useful alternative in vivo model for the assessment of chemicals interfering with the HPT system. (C) 2012 Elsevier Inc. All rights reserved.
关键词[WOS]: THYROTROPIN-BETA-SUBUNIT ; ENDOCRINE DISRUPTION ; GLAND ; GENE ; EXPRESSION ; PROMOTER ; ACTIVATION ; REGION ; BRAIN
语种: 英语
WOS记录号: WOS:000305845400006
ISSN号: 0041-008X
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/17015
Appears in Collections:水生生物分子与细胞生物学研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

Recommended Citation:
Ji, Cheng; Jin, Xia; He, Jiangyan; Yin, Zhan.Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals,TOXICOLOGY AND APPLIED PHARMACOLOGY,2012,262(2):149-155
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文件名: Use of TSH beta_EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals.pdf
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