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Involvement of nitric oxide in the mechanism of biochemical alterations induced by simulated microgravity in Microcystis aeruginosa
Xiao, Yuan1,2; Liu, Yongding1; Wang, Gaohong1; Liu, YD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Hubei Province, Peoples R China.
2012-03-01
Source PublicationADVANCES IN SPACE RESEARCH
ISSN0273-1177
Volume49Issue:5Pages:850-858
AbstractSimulated microgravity (SMG) can inhibit proliferation and enhance microcystin production of Microcystis aeruginosa. We investigated the role of nitric oxide (NO) in regulating the SMG induced changes of proliferation, photochemical system II photochemical activity, pigment, soluble protein and microcystin production in M. aeruginosa. M. aeruginosa was exposed to 0.1 mM sodium nitroprusside (SNP, NO donor) or 0.02 mM 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, NO scavenger) alone or in combination with SMG for 48 h. SMG and SNP inhibited the growth of M. aeruginosa while c-PTIO had no effect on cell number. As to yield, the negative effect of SMG was augmented by SNP and suppressed by c-PTIO. The intracellular concentrations of chlorophyll a, carotenoid, phycocyanin, soluble protein and microcystin were increased by SMG after 48 h. The effects of SMG on these metabolic processes could be enhanced by SNP and be partly eliminated by c-PTIO. Moreover, SNP and c-PTIO only functioned in these biochemical processes under SMG, unlike in the regulation of cell proliferation and yield. These results showed that the effects of SMG could be enhanced by adding exogenous NO and be mitigated by scavenging endogenous NO, revealing the involvement of NO in the changes in biochemistry processes induced by SMG in M. aeruginosa. (C) 2011 Published by Elsevier Ltd. on behalf of COSPAR.; Simulated microgravity (SMG) can inhibit proliferation and enhance microcystin production of Microcystis aeruginosa. We investigated the role of nitric oxide (NO) in regulating the SMG induced changes of proliferation, photochemical system II photochemical activity, pigment, soluble protein and microcystin production in M. aeruginosa. M. aeruginosa was exposed to 0.1 mM sodium nitroprusside (SNP, NO donor) or 0.02 mM 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, NO scavenger) alone or in combination with SMG for 48 h. SMG and SNP inhibited the growth of M. aeruginosa while c-PTIO had no effect on cell number. As to yield, the negative effect of SMG was augmented by SNP and suppressed by c-PTIO. The intracellular concentrations of chlorophyll a, carotenoid, phycocyanin, soluble protein and microcystin were increased by SMG after 48 h. The effects of SMG on these metabolic processes could be enhanced by SNP and be partly eliminated by c-PTIO. Moreover, SNP and c-PTIO only functioned in these biochemical processes under SMG, unlike in the regulation of cell proliferation and yield. These results showed that the effects of SMG could be enhanced by adding exogenous NO and be mitigated by scavenging endogenous NO, revealing the involvement of NO in the changes in biochemistry processes induced by SMG in M. aeruginosa. (C) 2011 Published by Elsevier Ltd. on behalf of COSPAR.
SubtypeArticle
KeywordSimulated Microgravity Nitric Oxide (No) Snp C-ptio Microcystis Aeruginosa
Department[Xiao, Yuan; Liu, Yongding; Wang, Gaohong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Xiao, Yuan] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
DOI10.1016/j.asr.2011.11.003
WOS HeadingsScience & Technology ; Physical Sciences
Funding OrganizationChinese Manned Space Flight Project ; Chinese Manned Space Flight Project ; Chinese Manned Space Flight Project ; Chinese Manned Space Flight Project
Indexed BySCI
Language英语
WOS Research AreaAstronomy & Astrophysics ; Geology ; Meteorology & Atmospheric Sciences
WOS SubjectAstronomy & Astrophysics ; Geosciences, Multidisciplinary ; Meteorology & Atmospheric Sciences
WOS IDWOS:000301610600004
WOS KeywordOXIDATIVE STRESS ; GREEN-ALGA ; CELL-DEATH ; GROWTH ; MICROALGAE ; RESPONSES ; TOXICITY ; SYNTHASE ; LEVEL
Funding OrganizationChinese Manned Space Flight Project ; Chinese Manned Space Flight Project ; Chinese Manned Space Flight Project ; Chinese Manned Space Flight Project
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16997
Collection水环境工程研究中心_期刊论文
Corresponding AuthorLiu, YD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Xiao, Yuan,Liu, Yongding,Wang, Gaohong,et al. Involvement of nitric oxide in the mechanism of biochemical alterations induced by simulated microgravity in Microcystis aeruginosa[J]. ADVANCES IN SPACE RESEARCH,2012,49(5):850-858.
APA Xiao, Yuan,Liu, Yongding,Wang, Gaohong,&Liu, YD .(2012).Involvement of nitric oxide in the mechanism of biochemical alterations induced by simulated microgravity in Microcystis aeruginosa.ADVANCES IN SPACE RESEARCH,49(5),850-858.
MLA Xiao, Yuan,et al."Involvement of nitric oxide in the mechanism of biochemical alterations induced by simulated microgravity in Microcystis aeruginosa".ADVANCES IN SPACE RESEARCH 49.5(2012):850-858.
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