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题名: Involvement of nitric oxide in the mechanism of biochemical alterations induced by simulated microgravity in Microcystis aeruginosa
作者: Xiao, Yuan1, 2; Liu, Yongding1; Wang, Gaohong1
通讯作者: Liu, YD (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, 7 S Donghu Rd, Wuhan 430072, Hubei Province, Peoples R China.
关键词: Simulated microgravity ; Nitric oxide (NO) ; SNP ; c-PTIO ; Microcystis aeruginosa
刊名: ADVANCES IN SPACE RESEARCH
发表日期: 2012-03-01
DOI: 10.1016/j.asr.2011.11.003
卷: 49, 期:5, 页:850-858
收录类别: SCI
文章类型: Article
部门归属: [Xiao, Yuan; Liu, Yongding; Wang, Gaohong] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Xiao, Yuan] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS标题词: Science & Technology ; Physical Sciences
资助者: Chinese Manned Space Flight Project
类目[WOS]: Astronomy & Astrophysics ; Geosciences, Multidisciplinary ; Meteorology & Atmospheric Sciences
研究领域[WOS]: Astronomy & Astrophysics ; Geology ; Meteorology & Atmospheric Sciences
摘要: Simulated microgravity (SMG) can inhibit proliferation and enhance microcystin production of Microcystis aeruginosa. We investigated the role of nitric oxide (NO) in regulating the SMG induced changes of proliferation, photochemical system II photochemical activity, pigment, soluble protein and microcystin production in M. aeruginosa. M. aeruginosa was exposed to 0.1 mM sodium nitroprusside (SNP, NO donor) or 0.02 mM 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, NO scavenger) alone or in combination with SMG for 48 h. SMG and SNP inhibited the growth of M. aeruginosa while c-PTIO had no effect on cell number. As to yield, the negative effect of SMG was augmented by SNP and suppressed by c-PTIO. The intracellular concentrations of chlorophyll a, carotenoid, phycocyanin, soluble protein and microcystin were increased by SMG after 48 h. The effects of SMG on these metabolic processes could be enhanced by SNP and be partly eliminated by c-PTIO. Moreover, SNP and c-PTIO only functioned in these biochemical processes under SMG, unlike in the regulation of cell proliferation and yield. These results showed that the effects of SMG could be enhanced by adding exogenous NO and be mitigated by scavenging endogenous NO, revealing the involvement of NO in the changes in biochemistry processes induced by SMG in M. aeruginosa. (C) 2011 Published by Elsevier Ltd. on behalf of COSPAR.
英文摘要: Simulated microgravity (SMG) can inhibit proliferation and enhance microcystin production of Microcystis aeruginosa. We investigated the role of nitric oxide (NO) in regulating the SMG induced changes of proliferation, photochemical system II photochemical activity, pigment, soluble protein and microcystin production in M. aeruginosa. M. aeruginosa was exposed to 0.1 mM sodium nitroprusside (SNP, NO donor) or 0.02 mM 2-(4-carboxyphenyl)-4, 4, 5, 5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO, NO scavenger) alone or in combination with SMG for 48 h. SMG and SNP inhibited the growth of M. aeruginosa while c-PTIO had no effect on cell number. As to yield, the negative effect of SMG was augmented by SNP and suppressed by c-PTIO. The intracellular concentrations of chlorophyll a, carotenoid, phycocyanin, soluble protein and microcystin were increased by SMG after 48 h. The effects of SMG on these metabolic processes could be enhanced by SNP and be partly eliminated by c-PTIO. Moreover, SNP and c-PTIO only functioned in these biochemical processes under SMG, unlike in the regulation of cell proliferation and yield. These results showed that the effects of SMG could be enhanced by adding exogenous NO and be mitigated by scavenging endogenous NO, revealing the involvement of NO in the changes in biochemistry processes induced by SMG in M. aeruginosa. (C) 2011 Published by Elsevier Ltd. on behalf of COSPAR.
关键词[WOS]: OXIDATIVE STRESS ; GREEN-ALGA ; CELL-DEATH ; GROWTH ; MICROALGAE ; RESPONSES ; TOXICITY ; SYNTHASE ; LEVEL
语种: 英语
WOS记录号: WOS:000301610600004
ISSN号: 0273-1177
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16997
Appears in Collections:水环境工程研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Fresh Water Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China

Recommended Citation:
Xiao, Yuan; Liu, Yongding; Wang, Gaohong.Involvement of nitric oxide in the mechanism of biochemical alterations induced by simulated microgravity in Microcystis aeruginosa,ADVANCES IN SPACE RESEARCH,2012,49(5):850-858
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