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Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae
Chen, Qi; Yu, Liqin; Yang, Lihua; Zhou, Bingsheng; Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2012-04-01
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume110Pages:141-148
AbstractPolybrominated diphenyl ethers (PBDEs) have the potential to disturb the thyroid endocrine system, but little is known of such effects or underlying mechanisms of BDE-209 in fish. In the present study, bioconcentration and metabolism of BDE-209 were investigated in zebrafish embryos exposed at concentrations of 0, 0.08, 0.38 and 1.92 mg/L in water until 14 days post-fertilization (dpf). Chemical analysis revealed that BDE-209 was accumulated in zebrafish larvae, while also metabolic products were detected, including octa- and nona-BDEs, with nona-BDEs being predominant. The exposure resulted in alterations of both triiodothyronine (T3) and thyroxine (T4) levels, indicating thyroid endocrine disruption. Gene transcription in the hypothalamic-pituitary-thyroid (HPT) axis was further examined, and the results showed that the genes encoding corticotrophin-releasing hormone (CRH) and thyroid-stimulating hormone (TSH beta) were transcriptionally significantly up-regulated. Genes involved in thyroid development (Pax8 and Nkx2.1) and synthesis (sodium/iodide symporter, NIS, thyroglobulin, TG) were also transcriptionally up-regulated. Up-regulation of mRNA for thyronine deiodinase (Dio1 and Dio2) and thyroid hormone receptors (TR alpha and TR beta) was also observed. However, the genes encoding proteins involved in TH transport (transthyretin, TTR)and metabolism (uridinediphosphate-glucuronosyl-transferase, UGT1ab) were transcriptionally significantly down-regulated. Furthermore, protein synthesis of TG was significantly up-regulated, while that of TTR was significantly reduced. These results suggest that the hypothalamic-pituitary-thyroid axis can be evaluated to determine thyroid endocrine disruption by BDE-209 in developing zebrafish larvae. (C) 2012 Elsevier B.V. All rights reserved.; Polybrominated diphenyl ethers (PBDEs) have the potential to disturb the thyroid endocrine system, but little is known of such effects or underlying mechanisms of BDE-209 in fish. In the present study, bioconcentration and metabolism of BDE-209 were investigated in zebrafish embryos exposed at concentrations of 0, 0.08, 0.38 and 1.92 mg/L in water until 14 days post-fertilization (dpf). Chemical analysis revealed that BDE-209 was accumulated in zebrafish larvae, while also metabolic products were detected, including octa- and nona-BDEs, with nona-BDEs being predominant. The exposure resulted in alterations of both triiodothyronine (T3) and thyroxine (T4) levels, indicating thyroid endocrine disruption. Gene transcription in the hypothalamic-pituitary-thyroid (HPT) axis was further examined, and the results showed that the genes encoding corticotrophin-releasing hormone (CRH) and thyroid-stimulating hormone (TSH beta) were transcriptionally significantly up-regulated. Genes involved in thyroid development (Pax8 and Nkx2.1) and synthesis (sodium/iodide symporter, NIS, thyroglobulin, TG) were also transcriptionally up-regulated. Up-regulation of mRNA for thyronine deiodinase (Dio1 and Dio2) and thyroid hormone receptors (TR alpha and TR beta) was also observed. However, the genes encoding proteins involved in TH transport (transthyretin, TTR)and metabolism (uridinediphosphate-glucuronosyl-transferase, UGT1ab) were transcriptionally significantly down-regulated. Furthermore, protein synthesis of TG was significantly up-regulated, while that of TTR was significantly reduced. These results suggest that the hypothalamic-pituitary-thyroid axis can be evaluated to determine thyroid endocrine disruption by BDE-209 in developing zebrafish larvae. (C) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordBde-209 Hypothalamic-pituitary-thyroid Axis Gene Transcription Bioconcentration And Metabolism Thyroid Endocrine Disruption Zebrafish Larvae
Department[Chen, Qi; Yu, Liqin; Yang, Lihua; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
DOI10.1016/j.aquatox.2012.01.008
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Nature Science Foundation of China[20890113]; Chinese Academy of Sciences[KCZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B131F01] ; National Nature Science Foundation of China[20890113]; Chinese Academy of Sciences[KCZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B131F01] ; National Nature Science Foundation of China[20890113]; Chinese Academy of Sciences[KCZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B131F01] ; National Nature Science Foundation of China[20890113]; Chinese Academy of Sciences[KCZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B131F01]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000302835900017
WOS KeywordPOLYBROMINATED DIPHENYL ETHERS ; PEARL RIVER DELTA ; SPRAGUE-DAWLEY RATS ; PIMEPHALES-PROMELAS ; HORMONE RECEPTORS ; IN-VITRO ; POLYCHLORINATED-BIPHENYLS ; DEVELOPMENTAL EXPOSURE ; GENE-EXPRESSION ; SOUTH CHINA
Funding OrganizationNational Nature Science Foundation of China[20890113]; Chinese Academy of Sciences[KCZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B131F01] ; National Nature Science Foundation of China[20890113]; Chinese Academy of Sciences[KCZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B131F01] ; National Nature Science Foundation of China[20890113]; Chinese Academy of Sciences[KCZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B131F01] ; National Nature Science Foundation of China[20890113]; Chinese Academy of Sciences[KCZCX2-YW-Q02-05]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B131F01]
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Cited Times:110[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16983
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
AffiliationChinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
Recommended Citation
GB/T 7714
Chen, Qi,Yu, Liqin,Yang, Lihua,et al. Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae[J]. AQUATIC TOXICOLOGY,2012,110:141-148.
APA Chen, Qi,Yu, Liqin,Yang, Lihua,Zhou, Bingsheng,&Zhou, BS .(2012).Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae.AQUATIC TOXICOLOGY,110,141-148.
MLA Chen, Qi,et al."Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae".AQUATIC TOXICOLOGY 110(2012):141-148.
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