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INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA
Cai, Linlin1,2; Zhou, Qiaohong1; He, Feng1; Kong, Lingwei1,2; Hu, Shenghua1; Wu, Zhenbin1; Wu, ZB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2012
Source PublicationFRESENIUS ENVIRONMENTAL BULLETIN
ISSN1018-4619
Volume21Issue:6Pages:1357-1364
AbstractCulture-dependent and independent PCR-DGGE methods were used to investigate the genetic diversity of microbial community in the western West Lake, Hangzhou. Water samples (n=7) were collected from the western West Lake in August, 2010. Crude DNA was directly extracted from water samples and mix-culture samples, and then used as the templates for the amplification of 16S rDNA V-3 region. The fingerprinting map of PCR-DGGE, sequencing and phylogenetic analysis all indicated that microbial communities were highly variable in the western West Lake and most microbes existed in very low abundance. The sublake Wuguitan had the highest microbial diversity (represented by 27 DGGE bands). The microbial communities of the physically isolated sub-lakes were similar in the western West Lake while the microbial communities dramatically changed at different sampling sites in the same sublake. The two dominant microorganisms detected by culture-independent PCR-DGGE are closely related to the uncultured bacterium HQ671854.1 and anaerobic bacterium ANA No.40 AB064556.1 while the most abundant microbes were found to be Acinetobacter sp. and Pseudomonas sp. by using culture dependent PCR-DGGE method.; Culture-dependent and independent PCR-DGGE methods were used to investigate the genetic diversity of microbial community in the western West Lake, Hangzhou. Water samples (n=7) were collected from the western West Lake in August, 2010. Crude DNA was directly extracted from water samples and mix-culture samples, and then used as the templates for the amplification of 16S rDNA V-3 region. The fingerprinting map of PCR-DGGE, sequencing and phylogenetic analysis all indicated that microbial communities were highly variable in the western West Lake and most microbes existed in very low abundance. The sublake Wuguitan had the highest microbial diversity (represented by 27 DGGE bands). The microbial communities of the physically isolated sub-lakes were similar in the western West Lake while the microbial communities dramatically changed at different sampling sites in the same sublake. The two dominant microorganisms detected by culture-independent PCR-DGGE are closely related to the uncultured bacterium HQ671854.1 and anaerobic bacterium ANA No.40 AB064556.1 while the most abundant microbes were found to be Acinetobacter sp. and Pseudomonas sp. by using culture dependent PCR-DGGE method.
SubtypeArticle
KeywordMicrobial Community Structure Culture Dependent And inDependent Pcr-dgge Western West Lake
Department[Cai, Linlin; Zhou, Qiaohong; He, Feng; Kong, Lingwei; Hu, Shenghua; Wu, Zhenbin] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Cai, Linlin; Kong, Lingwei] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Nature Science Foundation of China[50909091]; National Key Technologies R&D Program of China[2009ZX07209-004-4, 2012ZX07101007-005] ; National Nature Science Foundation of China[50909091]; National Key Technologies R&D Program of China[2009ZX07209-004-4, 2012ZX07101007-005] ; National Nature Science Foundation of China[50909091]; National Key Technologies R&D Program of China[2009ZX07209-004-4, 2012ZX07101007-005] ; National Nature Science Foundation of China[50909091]; National Key Technologies R&D Program of China[2009ZX07209-004-4, 2012ZX07101007-005]
Indexed BySCI
Language英语
WOS Research AreaEnvironmental Sciences & Ecology
WOS SubjectEnvironmental Sciences
WOS IDWOS:000305776800002
WOS KeywordGRADIENT GEL-ELECTROPHORESIS ; 16S RIBOSOMAL-RNA ; WASTE-WATER ; BACTERIAL ; BIOFILMS ; MICROORGANISMS ; BIOREACTORS ; DIVERSITY
Funding OrganizationNational Nature Science Foundation of China[50909091]; National Key Technologies R&D Program of China[2009ZX07209-004-4, 2012ZX07101007-005] ; National Nature Science Foundation of China[50909091]; National Key Technologies R&D Program of China[2009ZX07209-004-4, 2012ZX07101007-005] ; National Nature Science Foundation of China[50909091]; National Key Technologies R&D Program of China[2009ZX07209-004-4, 2012ZX07101007-005] ; National Nature Science Foundation of China[50909091]; National Key Technologies R&D Program of China[2009ZX07209-004-4, 2012ZX07101007-005]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16971
Collection水环境工程研究中心_期刊论文
Corresponding AuthorWu, ZB (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
Recommended Citation
GB/T 7714
Cai, Linlin,Zhou, Qiaohong,He, Feng,et al. INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA[J]. FRESENIUS ENVIRONMENTAL BULLETIN,2012,21(6):1357-1364.
APA Cai, Linlin.,Zhou, Qiaohong.,He, Feng.,Kong, Lingwei.,Hu, Shenghua.,...&Wu, ZB .(2012).INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA.FRESENIUS ENVIRONMENTAL BULLETIN,21(6),1357-1364.
MLA Cai, Linlin,et al."INVESTIGATION OF MICROBIAL COMMUNITY STRUCTURE WITH CULTURE-DEPENDENT AND INDEPENDENT PCR-DGGE METHODS IN WESTERN WEST LAKE OF HANGZHOU, CHINA".FRESENIUS ENVIRONMENTAL BULLETIN 21.6(2012):1357-1364.
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