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Alterations in retinoid status after long-term exposure to PBDEs in zebrafish (Danio rerio)
Chen, Lianguo1,2; Hu, Chenyan1; Huang, Changjiang3; Wang, Qiangwei1; Wang, Xiaofang1; Yang, Lihua1; Zhou, Bingsheng1; Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
2012-09-15
Source PublicationAQUATIC TOXICOLOGY
ISSN0166-445X
Volume120Pages:11-18
AbstractThis study examined the disruptive effect of exposure to polybrominated diphenyl ethers (PBDEs) on retinoid content in zebrafish (Danio rerio). Adult zebrafish were exposed to an environmentally relevant concentration (0.45 mu g/L) and a higher concentration (9.6 mu g/L) of DE-71 for 60 days. Retinoid content and gene transcription levels were examined in female zebrafish. PBDE exposure caused a significant decrease of retinyl ester content in the intestine and a downregulation of intestinal cellular retinal binding protein gene transcription (CRBP1a). In the liver, retinyl ester content was significantly decreased, while retinol content was increased. An upregulation of liver CRBP2a and retinal binding protein (RBP) gene transcription and an increased level of RBP protein were observed. In the eyes, both the retinal and retinyl ester content were increased and CRBPla gene transcription was upregulated. However, the gene encoding for retinal dehydrogenase (1RALDH2), responsible for retinoic acid synthesis, was downregulated in the eyes. CYP26a, the gene responsible for retinoic acid degradation, was upregulated, which indicated an increased level of retinoic acid. In the ovaries, the increased deposition of retinoids was also observed, while gene transcription levels of both CRBPs (CRBP1a and CRBP1b) were upregulated. An increased deposition of retinal was measured in the eggs. Overall, this study demonstrated that long-term exposure of zebrafish to environmentally relevant concentrations of DE-71 disrupted the transport, storage and metabolism of retinoid in various tissues. This study also indicated that retinoid levels in zebrafish are sensitive to PBDE exposure and highlighted the importance of liver storage, which appears to support important functions in reproduction and vision. (C) 2012 Elsevier B.V. All rights reserved.; This study examined the disruptive effect of exposure to polybrominated diphenyl ethers (PBDEs) on retinoid content in zebrafish (Danio rerio). Adult zebrafish were exposed to an environmentally relevant concentration (0.45 mu g/L) and a higher concentration (9.6 mu g/L) of DE-71 for 60 days. Retinoid content and gene transcription levels were examined in female zebrafish. PBDE exposure caused a significant decrease of retinyl ester content in the intestine and a downregulation of intestinal cellular retinal binding protein gene transcription (CRBP1a). In the liver, retinyl ester content was significantly decreased, while retinol content was increased. An upregulation of liver CRBP2a and retinal binding protein (RBP) gene transcription and an increased level of RBP protein were observed. In the eyes, both the retinal and retinyl ester content were increased and CRBPla gene transcription was upregulated. However, the gene encoding for retinal dehydrogenase (1RALDH2), responsible for retinoic acid synthesis, was downregulated in the eyes. CYP26a, the gene responsible for retinoic acid degradation, was upregulated, which indicated an increased level of retinoic acid. In the ovaries, the increased deposition of retinoids was also observed, while gene transcription levels of both CRBPs (CRBP1a and CRBP1b) were upregulated. An increased deposition of retinal was measured in the eggs. Overall, this study demonstrated that long-term exposure of zebrafish to environmentally relevant concentrations of DE-71 disrupted the transport, storage and metabolism of retinoid in various tissues. This study also indicated that retinoid levels in zebrafish are sensitive to PBDE exposure and highlighted the importance of liver storage, which appears to support important functions in reproduction and vision. (C) 2012 Elsevier B.V. All rights reserved.
SubtypeArticle
KeywordPbdes Retinoids Retinal Retinyl Ester Gene Transcription Zebrafish
Department[Chen, Lianguo; Hu, Chenyan; Wang, Qiangwei; Wang, Xiaofang; Yang, Lihua; Zhou, Bingsheng] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China; [Chen, Lianguo] Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China; [Huang, Changjiang] Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou 325035, Peoples R China
DOI10.1016/j.aquatox.2012.04.010
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B13] ; National Natural Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B13] ; National Natural Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B13] ; National Natural Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B13]
Indexed BySCI
Language英语
WOS Research AreaMarine & Freshwater Biology ; Toxicology
WOS SubjectMarine & Freshwater Biology ; Toxicology
WOS IDWOS:000306615500002
WOS KeywordPOLYBROMINATED DIPHENYL ETHERS ; VITAMIN-A STATUS ; POLYCHLORINATED-BIPHENYLS ; PHOTORECEPTOR DEVELOPMENT ; DECABROMODIPHENYL ETHER ; PIMEPHALES-PROMELAS ; FLAME RETARDANTS ; THYROID-HORMONE ; ACID ; ADULT
Funding OrganizationNational Natural Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B13] ; National Natural Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B13] ; National Natural Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B13] ; National Natural Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B13]
Citation statistics
Cited Times:22[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16967
Collection水环境工程研究中心_期刊论文
Corresponding AuthorZhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
3.Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou 325035, Peoples R China
Recommended Citation
GB/T 7714
Chen, Lianguo,Hu, Chenyan,Huang, Changjiang,et al. Alterations in retinoid status after long-term exposure to PBDEs in zebrafish (Danio rerio)[J]. AQUATIC TOXICOLOGY,2012,120:11-18.
APA Chen, Lianguo.,Hu, Chenyan.,Huang, Changjiang.,Wang, Qiangwei.,Wang, Xiaofang.,...&Zhou, BS .(2012).Alterations in retinoid status after long-term exposure to PBDEs in zebrafish (Danio rerio).AQUATIC TOXICOLOGY,120,11-18.
MLA Chen, Lianguo,et al."Alterations in retinoid status after long-term exposure to PBDEs in zebrafish (Danio rerio)".AQUATIC TOXICOLOGY 120(2012):11-18.
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