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First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China
Liu, J. Y.1,2; Li, A. H.1; Li, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
2012-05-01
Source PublicationJOURNAL OF FISH DISEASES
ISSN0140-7775
Volume35Issue:5Pages:335-342
AbstractAn epizootic in snakehead fish, Ophiocephalus argus, in earthen ponds in Xianning, Hubei Province, central China, from June to August 2009 was found to be caused by Aeromonas schubertii. The cumulative mortality within 40 days was 45%, and the diseased fish were 18 months old and 3545 cm in length. Multiple, ivory-white, firm nodules, 0.51 mm in diameter, were scattered throughout the kidney. Blood clots, 35 mm in diameter, were found in the liver. This is a disease frequently found in cultured snakehead throughout China. Isolated bacteria were Gram negative, facultatively anaerobic, motile, short rod-shaped, with a length of 0.31.0 mu m. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA, gyrB, rpoD and dnaJ gene sequencing all strongly indicated that these snakehead isolates are identical to A.similar to chubertii. In addition, the isolates possessed two plasmids: 5.0 kb and 10.0 kb. Antibiotic sensitivity testing of the isolates was carried out by the standard KirbyBauer disc diffusion method. Experimental infection assays were conducted, and pathogenicity (by intraperitoneal injection) was demonstrated in snakehead fingerlings and zebrafish, Brachydanio rerio (Hamilton).; An epizootic in snakehead fish, Ophiocephalus argus, in earthen ponds in Xianning, Hubei Province, central China, from June to August 2009 was found to be caused by Aeromonas schubertii. The cumulative mortality within 40 days was 45%, and the diseased fish were 18 months old and 3545 cm in length. Multiple, ivory-white, firm nodules, 0.51 mm in diameter, were scattered throughout the kidney. Blood clots, 35 mm in diameter, were found in the liver. This is a disease frequently found in cultured snakehead throughout China. Isolated bacteria were Gram negative, facultatively anaerobic, motile, short rod-shaped, with a length of 0.31.0 mu m. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA, gyrB, rpoD and dnaJ gene sequencing all strongly indicated that these snakehead isolates are identical to A.similar to chubertii. In addition, the isolates possessed two plasmids: 5.0 kb and 10.0 kb. Antibiotic sensitivity testing of the isolates was carried out by the standard KirbyBauer disc diffusion method. Experimental infection assays were conducted, and pathogenicity (by intraperitoneal injection) was demonstrated in snakehead fingerlings and zebrafish, Brachydanio rerio (Hamilton).
SubtypeArticle
KeywordAeromonas Schubertii China Infection Snakehead
Department[Liu, J. Y.; Li, A. H.] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liu, J. Y.] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
DOI10.1111/j.1365-2761.2012.01350.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705] ; National Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705] ; National Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705] ; National Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000302615000002
WOS KeywordGENUS AEROMONAS ; PHYLOGENETIC ANALYSIS ; NUCLEOTIDE-SEQUENCES ; ALIGNMENT ; STRAINS ; GYRB ; IDENTIFICATION ; HYBRIDIZATION ; NOCARDIOSIS ; PATHOGENS
Funding OrganizationNational Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705] ; National Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705] ; National Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705] ; National Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705]
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Cited Times:19[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16963
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLi, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Liu, J. Y.,Li, A. H.,Li, AH . First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China[J]. JOURNAL OF FISH DISEASES,2012,35(5):335-342.
APA Liu, J. Y.,Li, A. H.,&Li, AH .(2012).First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China.JOURNAL OF FISH DISEASES,35(5),335-342.
MLA Liu, J. Y.,et al."First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China".JOURNAL OF FISH DISEASES 35.5(2012):335-342.
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