中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China
作者: Liu, J. Y.1, 2; Li, A. H.1
通讯作者: Li, AH (reprint author), Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China.
关键词: Aeromonas schubertii ; China ; infection ; snakehead
刊名: JOURNAL OF FISH DISEASES
发表日期: 2012-05-01
DOI: 10.1111/j.1365-2761.2012.01350.x
卷: 35, 期:5, 页:335-342
收录类别: SCI
文章类型: Article
部门归属: [Liu, J. Y.; Li, A. H.] Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Liu, J. Y.] Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China[30670112, 31070112]; National Basic Research Program of China (973 Program)[2009CB118705]
类目[WOS]: Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]: Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences
摘要: An epizootic in snakehead fish, Ophiocephalus argus, in earthen ponds in Xianning, Hubei Province, central China, from June to August 2009 was found to be caused by Aeromonas schubertii. The cumulative mortality within 40 days was 45%, and the diseased fish were 18 months old and 3545 cm in length. Multiple, ivory-white, firm nodules, 0.51 mm in diameter, were scattered throughout the kidney. Blood clots, 35 mm in diameter, were found in the liver. This is a disease frequently found in cultured snakehead throughout China. Isolated bacteria were Gram negative, facultatively anaerobic, motile, short rod-shaped, with a length of 0.31.0 mu m. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA, gyrB, rpoD and dnaJ gene sequencing all strongly indicated that these snakehead isolates are identical to A.similar to chubertii. In addition, the isolates possessed two plasmids: 5.0 kb and 10.0 kb. Antibiotic sensitivity testing of the isolates was carried out by the standard KirbyBauer disc diffusion method. Experimental infection assays were conducted, and pathogenicity (by intraperitoneal injection) was demonstrated in snakehead fingerlings and zebrafish, Brachydanio rerio (Hamilton).
英文摘要: An epizootic in snakehead fish, Ophiocephalus argus, in earthen ponds in Xianning, Hubei Province, central China, from June to August 2009 was found to be caused by Aeromonas schubertii. The cumulative mortality within 40 days was 45%, and the diseased fish were 18 months old and 3545 cm in length. Multiple, ivory-white, firm nodules, 0.51 mm in diameter, were scattered throughout the kidney. Blood clots, 35 mm in diameter, were found in the liver. This is a disease frequently found in cultured snakehead throughout China. Isolated bacteria were Gram negative, facultatively anaerobic, motile, short rod-shaped, with a length of 0.31.0 mu m. Morphological and biochemical tests, as well as phylogenetic analysis derived from 16S rRNA, gyrB, rpoD and dnaJ gene sequencing all strongly indicated that these snakehead isolates are identical to A.similar to chubertii. In addition, the isolates possessed two plasmids: 5.0 kb and 10.0 kb. Antibiotic sensitivity testing of the isolates was carried out by the standard KirbyBauer disc diffusion method. Experimental infection assays were conducted, and pathogenicity (by intraperitoneal injection) was demonstrated in snakehead fingerlings and zebrafish, Brachydanio rerio (Hamilton).
关键词[WOS]: GENUS AEROMONAS ; PHYLOGENETIC ANALYSIS ; NUCLEOTIDE-SEQUENCES ; ALIGNMENT ; STRAINS ; GYRB ; IDENTIFICATION ; HYBRIDIZATION ; NOCARDIOSIS ; PATHOGENS
语种: 英语
WOS记录号: WOS:000302615000002
ISSN号: 0140-7775
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16963
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China.pdf(225KB)----开放获取--View 联系获取全文

作者单位: 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China

Recommended Citation:
Liu, J. Y.; Li, A. H..First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China,JOURNAL OF FISH DISEASES,2012,35(5):335-342
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Liu, J. Y.]'s Articles
[Li, A. H.]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Liu, J. Y.]‘s Articles
[Li, A. H.]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: First case of Aeromonas schubertii infection in the freshwater cultured snakehead fish, Ophiocephalus argus (Cantor), in China.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace