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C-Reactive Protein Induces Interleukin-6 and Thrombospondin-1 Protein and mRNA Expression through Activation of Nuclear Factor-kappa B in HK-2 Cells
Wang, Hai-rong1; Chen, De-liang1; Zhao, Mingming1; Shu, Shao-wu2; Xiong, Shi-xi1; Gan, Xue-dong1; Chao, Sheng-ping1; Cao, Jian-lei1; Wang, HR (reprint author), Wuhan Univ, Zhongnan Hosp, Dept Cardiol, 169 Dong Hu Rd, Wuhan 430071, Peoples R China.
2012
Source PublicationKIDNEY & BLOOD PRESSURE RESEARCH
ISSN1420-4096
Volume35Issue:4Pages:211-219
AbstractBackground: Although C-reactive protein (CRP) is significantly increased in patients with diabetic nephropathy, whether CRP exerts direct proinflammatory effects on human renal tubular epithelial cells (HK-2 cells) is still unclear. Methods: HK-2 cells were incubated with purified CRP at clinically relevant concentrations (0, 5, 10, 20 and 40 mu g/ml). The protein and transcript levels of thrombospondin-1 (TSP-1) and interleukin-6 (IL-6) were determined by ELISA and RT-PCR. Phosphorylation of p38MAPK was investigated through Western blot analysis in HK-2 cells induced by CRP. The activation of nuclear factor-kappa B (NF-kappa B) was studied via EMSA. A specific p38MAPK inhibitor (SB203580) and an NF-kappa B inhibitor (PDTC; pyrrolidine dithiocarbamate) were used to analyze the signal transduction in CRP induction. To explore the direct or indirect role of CRP in HK-2 cells, IL-6 or TSP-1 antibodies were used. The expression of IL-6, TSP-1 and transforming growth factor-beta(1) (TGF-beta(1)) were determined through Western blot analysis in HK-2 cells. Results: In HK-2 cells, purified CRP significantly induced protein release and mRNA expression of IL-6 and TSP-1 in a dose- and time-dependent manner. TGF-beta(1) protein was overexpressed in HK-2 cells induced by CRP, which cannot be inhibited by IL-6 or TSP-1 antibodies. CRP triggered phosphorylation of p38MAPK and activation of NF-kappa B-mediated signal transduction. SB203580 (5 mu m) and PDTC (50 mu m) efficiently suppressed those effects of CRP in HK-2 cells. Conclusions: CRP induces IL-6 and TSP-1 protein release and mRNA expression from HK-2 cells via activation of the p38MAPK and NF-kappa B signaling pathways and TGE-beta(1) was highly expressed in HK-2 cells, suggesting that CRP plays an important role in the propagation and prolongation of inflammation in renal fibrosis. Copyright (C) 2012 S. Karger AG, Basel; Background: Although C-reactive protein (CRP) is significantly increased in patients with diabetic nephropathy, whether CRP exerts direct proinflammatory effects on human renal tubular epithelial cells (HK-2 cells) is still unclear. Methods: HK-2 cells were incubated with purified CRP at clinically relevant concentrations (0, 5, 10, 20 and 40 mu g/ml). The protein and transcript levels of thrombospondin-1 (TSP-1) and interleukin-6 (IL-6) were determined by ELISA and RT-PCR. Phosphorylation of p38MAPK was investigated through Western blot analysis in HK-2 cells induced by CRP. The activation of nuclear factor-kappa B (NF-kappa B) was studied via EMSA. A specific p38MAPK inhibitor (SB203580) and an NF-kappa B inhibitor (PDTC; pyrrolidine dithiocarbamate) were used to analyze the signal transduction in CRP induction. To explore the direct or indirect role of CRP in HK-2 cells, IL-6 or TSP-1 antibodies were used. The expression of IL-6, TSP-1 and transforming growth factor-beta(1) (TGF-beta(1)) were determined through Western blot analysis in HK-2 cells. Results: In HK-2 cells, purified CRP significantly induced protein release and mRNA expression of IL-6 and TSP-1 in a dose- and time-dependent manner. TGF-beta(1) protein was overexpressed in HK-2 cells induced by CRP, which cannot be inhibited by IL-6 or TSP-1 antibodies. CRP triggered phosphorylation of p38MAPK and activation of NF-kappa B-mediated signal transduction. SB203580 (5 mu m) and PDTC (50 mu m) efficiently suppressed those effects of CRP in HK-2 cells. Conclusions: CRP induces IL-6 and TSP-1 protein release and mRNA expression from HK-2 cells via activation of the p38MAPK and NF-kappa B signaling pathways and TGE-beta(1) was highly expressed in HK-2 cells, suggesting that CRP plays an important role in the propagation and prolongation of inflammation in renal fibrosis. Copyright (C) 2012 S. Karger AG, Basel
SubtypeArticle
KeywordC-reactive Protein Renal Tubular Epithelial Cells Mitogen-activated Protein Kinase Nuclear Factor-kappa b Interleukin-6 Thrombospondin-1
Department[Wang, Hai-rong; Chen, De-liang; Zhao, Mingming; Xiong, Shi-xi; Gan, Xue-dong; Chao, Sheng-ping; Cao, Jian-lei] Wuhan Univ, Zhongnan Hosp, Dept Cardiol, Wuhan 430071, Peoples R China; [Shu, Shao-wu] State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
DOI10.1159/000332402
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationHubei Province Scientific Research Foundation[2007AA301B36-3, 2009CDB059]; Hubei Province Health Department Important Foundation[JX2A19]; State Key Laboratory of Freshwater Ecology and Biotechnology[2010FB12] ; Hubei Province Scientific Research Foundation[2007AA301B36-3, 2009CDB059]; Hubei Province Health Department Important Foundation[JX2A19]; State Key Laboratory of Freshwater Ecology and Biotechnology[2010FB12] ; Hubei Province Scientific Research Foundation[2007AA301B36-3, 2009CDB059]; Hubei Province Health Department Important Foundation[JX2A19]; State Key Laboratory of Freshwater Ecology and Biotechnology[2010FB12] ; Hubei Province Scientific Research Foundation[2007AA301B36-3, 2009CDB059]; Hubei Province Health Department Important Foundation[JX2A19]; State Key Laboratory of Freshwater Ecology and Biotechnology[2010FB12]
Indexed BySCI
Language英语
WOS Research AreaPhysiology ; Urology & Nephrology ; Cardiovascular System & Cardiology
WOS SubjectPhysiology ; Urology & Nephrology ; Peripheral Vascular Disease
WOS IDWOS:000303846100002
WOS KeywordTUMOR-NECROSIS-FACTOR ; SMOOTH-MUSCLE-CELLS ; IN-VITRO ; CARDIOVASCULAR-DISEASE ; ENDOTHELIAL-CELLS ; TNF-ALPHA ; INFLAMMATION ; MACROPHAGES ; INHIBITION ; GAMMA
Funding OrganizationHubei Province Scientific Research Foundation[2007AA301B36-3, 2009CDB059]; Hubei Province Health Department Important Foundation[JX2A19]; State Key Laboratory of Freshwater Ecology and Biotechnology[2010FB12] ; Hubei Province Scientific Research Foundation[2007AA301B36-3, 2009CDB059]; Hubei Province Health Department Important Foundation[JX2A19]; State Key Laboratory of Freshwater Ecology and Biotechnology[2010FB12] ; Hubei Province Scientific Research Foundation[2007AA301B36-3, 2009CDB059]; Hubei Province Health Department Important Foundation[JX2A19]; State Key Laboratory of Freshwater Ecology and Biotechnology[2010FB12] ; Hubei Province Scientific Research Foundation[2007AA301B36-3, 2009CDB059]; Hubei Province Health Department Important Foundation[JX2A19]; State Key Laboratory of Freshwater Ecology and Biotechnology[2010FB12]
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Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16961
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorWang, HR (reprint author), Wuhan Univ, Zhongnan Hosp, Dept Cardiol, 169 Dong Hu Rd, Wuhan 430071, Peoples R China.
Affiliation1.Wuhan Univ, Zhongnan Hosp, Dept Cardiol, Wuhan 430071, Peoples R China
2.State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Wang, Hai-rong,Chen, De-liang,Zhao, Mingming,et al. C-Reactive Protein Induces Interleukin-6 and Thrombospondin-1 Protein and mRNA Expression through Activation of Nuclear Factor-kappa B in HK-2 Cells[J]. KIDNEY & BLOOD PRESSURE RESEARCH,2012,35(4):211-219.
APA Wang, Hai-rong.,Chen, De-liang.,Zhao, Mingming.,Shu, Shao-wu.,Xiong, Shi-xi.,...&Wang, HR .(2012).C-Reactive Protein Induces Interleukin-6 and Thrombospondin-1 Protein and mRNA Expression through Activation of Nuclear Factor-kappa B in HK-2 Cells.KIDNEY & BLOOD PRESSURE RESEARCH,35(4),211-219.
MLA Wang, Hai-rong,et al."C-Reactive Protein Induces Interleukin-6 and Thrombospondin-1 Protein and mRNA Expression through Activation of Nuclear Factor-kappa B in HK-2 Cells".KIDNEY & BLOOD PRESSURE RESEARCH 35.4(2012):211-219.
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