IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis
Cao, Hong1; Leng, Xiaoqian2; Li, Chuangju3; Wei, Qiwei3; Gui, Jianfang2; Cheng, Hanhua1; Zhou, Rongjia1; Cheng, HH (reprint author), Wuhan Univ, Dept Genet, Coll Life Sci, Wuhan 430072, Peoples R China.
2012-04-01
Source PublicationMOLECULAR BIOLOGY REPORTS
ISSN0301-4851
Volume39Issue:4Pages:4647-4653
AbstractChinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon.; Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon.
SubtypeArticle
KeywordEndocrinology Ests Somatolactin Acipenser Sinensis Pituitary
Department[Cao, Hong; Cheng, Hanhua; Zhou, Rongjia] Wuhan Univ, Dept Genet, Coll Life Sci, Wuhan 430072, Peoples R China; [Leng, Xiaoqian; Gui, Jianfang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Li, Chuangju; Wei, Qiwei] Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist China, Yangtze River Fisheries Res Inst, Jinzhou 434000, Peoples R China
DOI10.1007/s11033-011-1256-6
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Natural Science Foundation of China; National Key Basic Research project[2010CB126306] ; National Natural Science Foundation of China; National Key Basic Research project[2010CB126306] ; National Natural Science Foundation of China; National Key Basic Research project[2010CB126306] ; National Natural Science Foundation of China; National Key Basic Research project[2010CB126306]
Indexed BySCI
Language英语
WOS Research AreaBiochemistry & Molecular Biology
WOS SubjectBiochemistry & Molecular Biology
WOS IDWOS:000301108500146
WOS KeywordGROWTH-HORMONE ; CDNA CLONING ; COHO SALMON ; ONCORHYNCHUS-KISUTCH ; EXPRESSION PROFILE ; FISH ; PROLACTIN ; SEQUENCE ; CATFISH ; ALPHA
Funding OrganizationNational Natural Science Foundation of China; National Key Basic Research project[2010CB126306] ; National Natural Science Foundation of China; National Key Basic Research project[2010CB126306] ; National Natural Science Foundation of China; National Key Basic Research project[2010CB126306] ; National Natural Science Foundation of China; National Key Basic Research project[2010CB126306]
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16955
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorCheng, HH (reprint author), Wuhan Univ, Dept Genet, Coll Life Sci, Wuhan 430072, Peoples R China.
Affiliation1.Wuhan Univ, Dept Genet, Coll Life Sci, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist China, Yangtze River Fisheries Res Inst, Jinzhou 434000, Peoples R China
Recommended Citation
GB/T 7714
Cao, Hong,Leng, Xiaoqian,Li, Chuangju,et al. EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis[J]. MOLECULAR BIOLOGY REPORTS,2012,39(4):4647-4653.
APA Cao, Hong.,Leng, Xiaoqian.,Li, Chuangju.,Wei, Qiwei.,Gui, Jianfang.,...&Cheng, HH .(2012).EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis.MOLECULAR BIOLOGY REPORTS,39(4),4647-4653.
MLA Cao, Hong,et al."EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis".MOLECULAR BIOLOGY REPORTS 39.4(2012):4647-4653.
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