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题名: EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis
作者: Cao, Hong1; Leng, Xiaoqian2; Li, Chuangju3; Wei, Qiwei3; Gui, Jianfang2; Cheng, Hanhua1; Zhou, Rongjia1
通讯作者: Cheng, HH (reprint author), Wuhan Univ, Dept Genet, Coll Life Sci, Wuhan 430072, Peoples R China.
关键词: Endocrinology ; ESTs ; Somatolactin ; Acipenser sinensis ; Pituitary
刊名: MOLECULAR BIOLOGY REPORTS
发表日期: 2012-04-01
DOI: 10.1007/s11033-011-1256-6
卷: 39, 期:4, 页:4647-4653
收录类别: SCI
文章类型: Article
部门归属: [Cao, Hong; Cheng, Hanhua; Zhou, Rongjia] Wuhan Univ, Dept Genet, Coll Life Sci, Wuhan 430072, Peoples R China; [Leng, Xiaoqian; Gui, Jianfang] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China; [Li, Chuangju; Wei, Qiwei] Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist China, Yangtze River Fisheries Res Inst, Jinzhou 434000, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: National Natural Science Foundation of China; National Key Basic Research project[2010CB126306]
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
摘要: Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon.
英文摘要: Chinese sturgeon (Acipenser sinensis) is a rare and endangered species and also an important resource for the sturgeon aquaculture industry, however, a few genes have been identified in this species. We report here construction of a pituitary cDNA library from a 24 years old female Chinese sturgeon just after its spawning, and obtained 2,025 ESTs from the library. 885 unique sequences were identified, which were categorized into 12 functional groups. More than half of the unique sequences (57%) do not match with annotated sequences in the public databases. Three of these novel genes were further identified. Notably, a full-length of cDNA (1,143 bp) encoding somatolactin of 232 amino acids was identified. Phylogenetic analysis showed 97% amino acid identity with White sturgeon somatolactin. RT-PCR analysis indicated that the somatolactin mRNA was only detected in pituitary. Pituitary-specific expression of the somatolactin suggested that the protein may play important physiological functions in pituitary-endocrine system of the Chinese sturgeon.
关键词[WOS]: GROWTH-HORMONE ; CDNA CLONING ; COHO SALMON ; ONCORHYNCHUS-KISUTCH ; EXPRESSION PROFILE ; FISH ; PROLACTIN ; SEQUENCE ; CATFISH ; ALPHA
语种: 英语
WOS记录号: WOS:000301108500146
ISSN号: 0301-4851
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16955
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Wuhan Univ, Dept Genet, Coll Life Sci, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
3.Chinese Acad Fishery Sci, Key Lab Freshwater Biodivers Conservat & Utilizat, Minist China, Yangtze River Fisheries Res Inst, Jinzhou 434000, Peoples R China

Recommended Citation:
Cao, Hong; Leng, Xiaoqian; Li, Chuangju; Wei, Qiwei; Gui, Jianfang; Cheng, Hanhua; Zhou, Rongjia.EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis,MOLECULAR BIOLOGY REPORTS,2012,39(4):4647-4653
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文件名: EST dataset of pituitary and identification of somatolactin and novel genes in Chinese sturgeon, Acipenser sinensis.pdf
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