IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Identification of immunogenic proteins of Flavobacterium columnare by two-dimensional electrophoresis immunoblotting with antibacterial sera from grass carp, Ctenopharyngodon idella (Valenciennes)
Liu, Z. X.1; Liu, G. Y.2; Li, N.1; Xiao, F. S.1; Xie, H. X.1; Nie, P.1; Nie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
2012-04-01
Source PublicationJOURNAL OF FISH DISEASES
ISSN0140-7775
Volume35Issue:4Pages:255-263
AbstractFlavobacterium columnare is a Gram-negative bacterium causing columnaris disease of freshwater fish worldwide, and development of efficacious vaccines has been a continuous challenge in aquaculture. In this study, 14 proteins were identified from cellular components of F.similar to columnare using an immunoblotting approach in two-dimensional electrophoresis map gels with antibacterial sera from grass carp, Ctenopharyngodon idella (Valenciennes), and then anti-grass carp-recombinant Ig (rIg) polyclonal antibodies. These proteins were characterized conclusively by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF/TOF MS). The 14 proteins are immunogenic molecules of F.similar to columnare, including chaperonins DnaK, GroEL and trigger factor, and translation elongation factor G, translation elongation factor Tu, 30S ribosomal subunit protein S1, dihydrolipoamide succinyltransferase, succinyl-CoA synthetase, SpoOJ regulator protein, alcohol dehydrogenase, fructose-bisphosphate aldolase, 3-hydroxybutyryl-CoA dehydrogenase and two conserved hypothetical proteins. These identified immunogenic proteins may provide candidate molecules for the development of vaccines against columnaris disease.; Flavobacterium columnare is a Gram-negative bacterium causing columnaris disease of freshwater fish worldwide, and development of efficacious vaccines has been a continuous challenge in aquaculture. In this study, 14 proteins were identified from cellular components of F.similar to columnare using an immunoblotting approach in two-dimensional electrophoresis map gels with antibacterial sera from grass carp, Ctenopharyngodon idella (Valenciennes), and then anti-grass carp-recombinant Ig (rIg) polyclonal antibodies. These proteins were characterized conclusively by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF/TOF MS). The 14 proteins are immunogenic molecules of F.similar to columnare, including chaperonins DnaK, GroEL and trigger factor, and translation elongation factor G, translation elongation factor Tu, 30S ribosomal subunit protein S1, dihydrolipoamide succinyltransferase, succinyl-CoA synthetase, SpoOJ regulator protein, alcohol dehydrogenase, fructose-bisphosphate aldolase, 3-hydroxybutyryl-CoA dehydrogenase and two conserved hypothetical proteins. These identified immunogenic proteins may provide candidate molecules for the development of vaccines against columnaris disease.
SubtypeArticle
KeywordFlavobacterium Columnare Grass Carp Immunoblotting Immunogenic Protein
Department[Liu, Z. X.; Li, N.; Xiao, F. S.; Xie, H. X.; Nie, P.] Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China; [Liu, G. Y.] Huazhong Agr Univ, Coll Fisheries, Wuhan, Hubei Province, Peoples R China
DOI10.1111/j.1365-2761.2011.01340.x
WOS HeadingsScience & Technology ; Life Sciences & Biomedicine
Funding OrganizationNational Basic Research Program of China (973 Program)[2009CB118703] ; National Basic Research Program of China (973 Program)[2009CB118703] ; National Basic Research Program of China (973 Program)[2009CB118703] ; National Basic Research Program of China (973 Program)[2009CB118703]
Indexed BySCI
Language英语
WOS Research AreaFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS SubjectFisheries ; Marine & Freshwater Biology ; Veterinary Sciences
WOS IDWOS:000301343100002
WOS KeywordCHANNEL CATFISH ; IMMUNOPROTEOMIC ANALYSIS ; ICTALURUS-PUNCTATUS ; PROTEOME ANALYSIS ; CLONING ; GENE ; FISH ; IMMUNIZATION ; RECOMBINANT ; EXPRESSION
Funding OrganizationNational Basic Research Program of China (973 Program)[2009CB118703] ; National Basic Research Program of China (973 Program)[2009CB118703] ; National Basic Research Program of China (973 Program)[2009CB118703] ; National Basic Research Program of China (973 Program)[2009CB118703]
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16953
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorNie, P (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China.
Affiliation1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei Province, Peoples R China
2.Huazhong Agr Univ, Coll Fisheries, Wuhan, Hubei Province, Peoples R China
Recommended Citation
GB/T 7714
Liu, Z. X.,Liu, G. Y.,Li, N.,et al. Identification of immunogenic proteins of Flavobacterium columnare by two-dimensional electrophoresis immunoblotting with antibacterial sera from grass carp, Ctenopharyngodon idella (Valenciennes)[J]. JOURNAL OF FISH DISEASES,2012,35(4):255-263.
APA Liu, Z. X..,Liu, G. Y..,Li, N..,Xiao, F. S..,Xie, H. X..,...&Nie, P .(2012).Identification of immunogenic proteins of Flavobacterium columnare by two-dimensional electrophoresis immunoblotting with antibacterial sera from grass carp, Ctenopharyngodon idella (Valenciennes).JOURNAL OF FISH DISEASES,35(4),255-263.
MLA Liu, Z. X.,et al."Identification of immunogenic proteins of Flavobacterium columnare by two-dimensional electrophoresis immunoblotting with antibacterial sera from grass carp, Ctenopharyngodon idella (Valenciennes)".JOURNAL OF FISH DISEASES 35.4(2012):255-263.
Files in This Item:
File Name/Size DocType Version Access License
Identification of im(319KB) 开放获取--View Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Liu, Z. X.]'s Articles
[Liu, G. Y.]'s Articles
[Li, N.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Liu, Z. X.]'s Articles
[Liu, G. Y.]'s Articles
[Li, N.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Liu, Z. X.]'s Articles
[Liu, G. Y.]'s Articles
[Li, N.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Identification of immunogenic proteins of Flavobacterium.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.