IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway
Li, Shun; Sun, Fan; Zhang, Yi-Bing; Gui, Jian-Fang; Zhang, Qi-Ya; Li, S (reprint author), Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan, Peoples R China.
2012-03-07
Source PublicationPLOS ONE
ISSN1932-6203
Volume7Issue:3Pages:e32427
AbstractBackground: Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) had been demonstrated to prime interferon (IFN) response against viral infection via the conserved RLR signaling in fish, and a novel fish-specific gene, the grass carp reovirus (GCRV)-induced gene 2 (Gig2), had been suggested to play important role in host antiviral response. Methodology/Principal Findings: In this study, we cloned and characterized zebrafish Gig2 homolog (named Danio rerio Gig2-I, DreI), and revealed its antiviral role and expressional regulation signaling pathway. RT-PCR, Western blot and promoter activity assay indicate that DreI can be induced by poly I: C, spring viremia of carp virus (SVCV) and recombinant IFN (rIFN), showing that DreI is a typical ISG. Using the pivotal signaling molecules of RLR pathway, including RIG-I, MDA5 and IRF3 from crucian carp, it is found that DreI expression is regulated by RLR cascade and IRF3 plays an important role in this regulation. Furthermore, promoter mutation assay confirms that the IFN-stimulated regulatory elements (ISRE) in the 59 flanking region of DreI is essential for its induction. Finally, overexpression of DreI leads to establish a strong antiviral state against SVCV and Rana grylio virus (RGV) infection in EPC (Epithelioma papulosum cyprinid) cells. Conclusions/Significance: These data indicate that DreI is an antiviral protein, which is regulated by RLR signaling pathway.; Background: Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) had been demonstrated to prime interferon (IFN) response against viral infection via the conserved RLR signaling in fish, and a novel fish-specific gene, the grass carp reovirus (GCRV)-induced gene 2 (Gig2), had been suggested to play important role in host antiviral response.
SubtypeArticle
KeywordHepatitis-c-virus Trout Oncorhynchus-mykiss Dependent Protein-kinase Ifn-beta Promoter I-like Receptors Rig-i Functional-characterization Paralichthys-olivaceus Spring Viremia Carp Virus
Department[Li, Shun; Sun, Fan; Zhang, Yi-Bing; Gui, Jian-Fang; Zhang, Qi-Ya] Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan, Peoples R China
DOI10.1371/journal.pone.0032427
WOS HeadingsScience & Technology
Funding OrganizationNational Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16]
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000303060800019
WOS KeywordHEPATITIS-C-VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; DEPENDENT PROTEIN-KINASE ; IFN-BETA PROMOTER ; I-LIKE RECEPTORS ; RIG-I ; FUNCTIONAL-CHARACTERIZATION ; PARALICHTHYS-OLIVACEUS ; SPRING VIREMIA ; CARP VIRUS
Funding OrganizationNational Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16] ; National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16]
Citation statistics
Cited Times:23[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.ihb.ac.cn/handle/342005/16947
Collection鱼类生物学及渔业生物技术研究中心_期刊论文
Corresponding AuthorLi, S (reprint author), Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan, Peoples R China.
AffiliationChinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan, Peoples R China
Recommended Citation
GB/T 7714
Li, Shun,Sun, Fan,Zhang, Yi-Bing,et al. Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway[J]. PLOS ONE,2012,7(3):e32427.
APA Li, Shun,Sun, Fan,Zhang, Yi-Bing,Gui, Jian-Fang,Zhang, Qi-Ya,&Li, S .(2012).Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway.PLOS ONE,7(3),e32427.
MLA Li, Shun,et al."Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway".PLOS ONE 7.3(2012):e32427.
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