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题名: Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway
作者: Li, Shun1; Sun, Fan1; Zhang, Yi-Bing1; Gui, Jian-Fang1; Zhang, Qi-Ya1
通讯作者: Li, S (reprint author), Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan, Peoples R China.
关键词: HEPATITIS-C-VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; DEPENDENT PROTEIN-KINASE ; IFN-BETA PROMOTER ; I-LIKE RECEPTORS ; RIG-I ; FUNCTIONAL-CHARACTERIZATION ; PARALICHTHYS-OLIVACEUS ; SPRING VIREMIA ; CARP VIRUS
刊名: PLOS ONE
发表日期: 2012-03-07
DOI: 10.1371/journal.pone.0032427
卷: 7, 期:3, 页:e32427
收录类别: SCI
文章类型: Article
部门归属: [Li, Shun; Sun, Fan; Zhang, Yi-Bing; Gui, Jian-Fang; Zhang, Qi-Ya] Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan, Peoples R China
WOS标题词: Science & Technology
资助者: National Major Basic Research Program[2009CB118704, 2010CB126303]; Chinese Academy of Sciences[KSCX2-EW-Z-3]; National Natural Science Foundation of China[30871938, 31072239]; State Key Laboratory of Freshwater Ecology and Biotechnology (FEBL)[2008FBZ15, 2008FBZ16]
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
摘要: Background: Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) had been demonstrated to prime interferon (IFN) response against viral infection via the conserved RLR signaling in fish, and a novel fish-specific gene, the grass carp reovirus (GCRV)-induced gene 2 (Gig2), had been suggested to play important role in host antiviral response. Methodology/Principal Findings: In this study, we cloned and characterized zebrafish Gig2 homolog (named Danio rerio Gig2-I, DreI), and revealed its antiviral role and expressional regulation signaling pathway. RT-PCR, Western blot and promoter activity assay indicate that DreI can be induced by poly I: C, spring viremia of carp virus (SVCV) and recombinant IFN (rIFN), showing that DreI is a typical ISG. Using the pivotal signaling molecules of RLR pathway, including RIG-I, MDA5 and IRF3 from crucian carp, it is found that DreI expression is regulated by RLR cascade and IRF3 plays an important role in this regulation. Furthermore, promoter mutation assay confirms that the IFN-stimulated regulatory elements (ISRE) in the 59 flanking region of DreI is essential for its induction. Finally, overexpression of DreI leads to establish a strong antiviral state against SVCV and Rana grylio virus (RGV) infection in EPC (Epithelioma papulosum cyprinid) cells. Conclusions/Significance: These data indicate that DreI is an antiviral protein, which is regulated by RLR signaling pathway.
英文摘要: Background: Retinoic acid-inducible gene I (RIG-I)-like receptors (RLRs) had been demonstrated to prime interferon (IFN) response against viral infection via the conserved RLR signaling in fish, and a novel fish-specific gene, the grass carp reovirus (GCRV)-induced gene 2 (Gig2), had been suggested to play important role in host antiviral response.
关键词[WOS]: HEPATITIS-C-VIRUS ; TROUT ONCORHYNCHUS-MYKISS ; DEPENDENT PROTEIN-KINASE ; IFN-BETA PROMOTER ; I-LIKE RECEPTORS ; RIG-I ; FUNCTIONAL-CHARACTERIZATION ; PARALICHTHYS-OLIVACEUS ; SPRING VIREMIA ; CARP VIRUS
语种: 英语
WOS记录号: WOS:000303060800019
ISSN号: 1932-6203
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16947
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

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作者单位: 1.Chinese Acad Sci, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan Ctr Dev Biol,Inst Hydrobiol, Wuhan, Peoples R China

Recommended Citation:
Li, Shun; Sun, Fan; Zhang, Yi-Bing; Gui, Jian-Fang; Zhang, Qi-Ya.Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway,PLOS ONE,2012,7(3):e32427
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文件名: Identification of DreI as an Antiviral Factor Regulated by RLR Signaling Pathway.pdf
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