| Composition, Diversity, and Origin of the Bacterial Community in Grass Carp Intestine | |
| Wu, Shangong1,2; Wang, Guitang1,2; Angert, Esther R.3; Wang, Weiwei1,2; Li, Wenxiang1,2; Zou, Hong1,2; Wu, SG (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China. | |
| 2012-02-20 | |
| Source Publication | PLOS ONE
 |
| ISSN | 1932-6203 |
| Volume | 7Issue:2Pages:e30440 |
| Abstract | Gut microbiota has become an integral component of the host, and received increasing attention. However, for many domestic animals, information on the microbiota is insufficient and more effort should be exerted to manage the gastrointestinal bacterial community. Understanding the factors that influence the composition of microbial community in the host alimentary canal is essential to manage or improve the microbial community composition. In the present study, 16S rRNA gene sequence-based comparisons of the bacterial communities in the grass carp (Ctenopharyngodon idellus) intestinal contents and fish culture-associated environments are performed. The results show that the fish intestinal microbiota harbors many cellulose-decomposing bacteria, including sequences related to Anoxybacillus, Leuconostoc, Clostridium, Actinomyces, and Citrobacter. The most abundant bacterial operational taxonomic units (OTUs) in the grass carp intestinal content are those related to feed digestion. In addition, the potential pathogens and probiotics are important members of the intestinal microbiota. Further analyses show that grass carp intestine holds a core microbiota composed of Proteobacteria, Firmicutes, and Actinobacteria. The comparison analyses reveal that the bacterial community in the intestinal contents is most similar to those from the culture water and sediment. However, feed also plays significant influence on the composition of gut microbiota.; Gut microbiota has become an integral component of the host, and received increasing attention. However, for many domestic animals, information on the microbiota is insufficient and more effort should be exerted to manage the gastrointestinal bacterial community. Understanding the factors that influence the composition of microbial community in the host alimentary canal is essential to manage or improve the microbial community composition. In the present study, 16S rRNA gene sequence-based comparisons of the bacterial communities in the grass carp (Ctenopharyngodon idellus) intestinal contents and fish culture-associated environments are performed. The results show that the fish intestinal microbiota harbors many cellulose-decomposing bacteria, including sequences related to Anoxybacillus, Leuconostoc, Clostridium, Actinomyces, and Citrobacter. The most abundant bacterial operational taxonomic units (OTUs) in the grass carp intestinal content are those related to feed digestion. In addition, the potential pathogens and probiotics are important members of the intestinal microbiota. Further analyses show that grass carp intestine holds a core microbiota composed of Proteobacteria, Firmicutes, and Actinobacteria. The comparison analyses reveal that the bacterial community in the intestinal contents is most similar to those from the culture water and sediment. However, feed also plays significant influence on the composition of gut microbiota. |
| Subtype | Article |
| Keyword | Gut Microbiota Aeromonas-salmonicida Oncorhynchus-mykiss Probiotic Activity Rainbow-trout Fecal Samples Microflora Rumen Fish Pcr |
| Department | [Wu, Shangong; Wang, Guitang; Wang, Weiwei; Li, Wenxiang; Zou, Hong] Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China; [Wu, Shangong; Wang, Guitang; Wang, Weiwei; Li, Wenxiang; Zou, Hong] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China; [Angert, Esther R.] Cornell Univ, Dept Microbiol, Ithaca, NY USA |
| DOI | 10.1371/journal.pone.0030440 |
| WOS Headings | Science & Technology |
| Funding Organization | National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Science & Technology - Other Topics |
| WOS Subject | Multidisciplinary Sciences |
| WOS ID | WOS:000302871500008 |
| WOS Keyword | GUT MICROBIOTA ; AEROMONAS-SALMONICIDA ; ONCORHYNCHUS-MYKISS ; PROBIOTIC ACTIVITY ; RAINBOW-TROUT ; FECAL SAMPLES ; MICROFLORA ; RUMEN ; FISH ; PCR |
| Funding Organization | National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] ; National Basic Research Program of China[2009CB118705]; State Key Laboratory of Freshwater Ecology and Biotechnology[2011FB07]; earmarked fund for China Agriculture Research System[CARS-46-08] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/16943 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Wu, SG (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Wuhan, Peoples R China 2.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan, Peoples R China 3.Cornell Univ, Dept Microbiol, Ithaca, NY USA |
| Recommended Citation GB/T 7714 | Wu, Shangong,Wang, Guitang,Angert, Esther R.,et al. Composition, Diversity, and Origin of the Bacterial Community in Grass Carp Intestine[J]. PLOS ONE,2012,7(2):e30440. |
| APA | Wu, Shangong.,Wang, Guitang.,Angert, Esther R..,Wang, Weiwei.,Li, Wenxiang.,...&Wu, SG .(2012).Composition, Diversity, and Origin of the Bacterial Community in Grass Carp Intestine.PLOS ONE,7(2),e30440. |
| MLA | Wu, Shangong,et al."Composition, Diversity, and Origin of the Bacterial Community in Grass Carp Intestine".PLOS ONE 7.2(2012):e30440. |
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