| Identification of differentially expressed genes in Oncomelania hupensis chronically infected with Schistosoma japonicum | |
| Wang, Hao2; Zhao, Qin Ping1,2; Nie, Pin1; Sen Jiang, Ming2; Song, Jian2; Nie, P (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China. | |
| 2012-04-01 | |
| Source Publication | EXPERIMENTAL PARASITOLOGY
 |
| ISSN | 0014-4894 |
| Volume | 130Issue:4Pages:374-383 |
| Abstract | Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum. The schistosome-snail interaction is biomedically important. To identify differentially expressed transcripts in O. hupensis chronically infected with S. japonicum, suppression subtractive hybridization (SSH) was used to construct a cDNA library in each direction for transcripts that are more abundantly enriched in head-foot part of the infected O. hupensis and for those that are more abundantly enriched in the uninfected, as head-foot part contains hemocytes and hemolymph which are associated with the snail internal defense system. After differential screening, 39 transcripts were identified, including nine and 30 transcripts enriched in infected and uninfected snails, respectively. Some of the transcripts have similar homology to available sequences in current databases, including transposase, caveolin-like protein, pancreatic trypsin inhibitor-like protein, prosaposin, glutathione s-transferase (GST), and several hypothetical proteins, while most of the transcripts do not match with any sequences in available databases. The identified transcripts were involved functionally in cell growth, metabolism, signal transduction, and immune responses. Two forward library transcripts and 11 reverse library transcripts were selected for real-time PCR, and 10 of them were confirmed to be consistent with the SSH results. It is intriguing to continue functional studies for some genes such as pancreatic trypsin inhibitor; a hypothetical protein (HS576367) related to calcium ion binding; GST; and several unknown proteins (HS576353 and HS576355). These identified differentially expressed genes may be key targets for understanding the molecular mechanism of co-existence during which the snail is unable to rid itself of the schistosome in chronic infection stage. (C) 2012 Elsevier Inc. All rights reserved.; Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum. The schistosome-snail interaction is biomedically important. To identify differentially expressed transcripts in O. hupensis chronically infected with S. japonicum, suppression subtractive hybridization (SSH) was used to construct a cDNA library in each direction for transcripts that are more abundantly enriched in head-foot part of the infected O. hupensis and for those that are more abundantly enriched in the uninfected, as head-foot part contains hemocytes and hemolymph which are associated with the snail internal defense system. After differential screening, 39 transcripts were identified, including nine and 30 transcripts enriched in infected and uninfected snails, respectively. Some of the transcripts have similar homology to available sequences in current databases, including transposase, caveolin-like protein, pancreatic trypsin inhibitor-like protein, prosaposin, glutathione s-transferase (GST), and several hypothetical proteins, while most of the transcripts do not match with any sequences in available databases. The identified transcripts were involved functionally in cell growth, metabolism, signal transduction, and immune responses. Two forward library transcripts and 11 reverse library transcripts were selected for real-time PCR, and 10 of them were confirmed to be consistent with the SSH results. It is intriguing to continue functional studies for some genes such as pancreatic trypsin inhibitor; a hypothetical protein (HS576367) related to calcium ion binding; GST; and several unknown proteins (HS576353 and HS576355). These identified differentially expressed genes may be key targets for understanding the molecular mechanism of co-existence during which the snail is unable to rid itself of the schistosome in chronic infection stage. (C) 2012 Elsevier Inc. All rights reserved. |
| Subtype | Article |
| Keyword | Oncomelania Hupensis Suppression Subtractive Hybridization Schistosoma Japonicum Chronic Infection Schistosome-snail Interaction Head-foot |
| Department | [Zhao, Qin Ping; Nie, Pin] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Wang, Hao; Zhao, Qin Ping; Sen Jiang, Ming; Song, Jian] Wuhan Univ, Sch Basic Med Sci, Wuhan 430071, Hubei Province, Peoples R China |
| DOI | 10.1016/j.exppara.2012.02.004 |
| WOS Headings | Science & Technology ; Life Sciences & Biomedicine |
| Funding Organization | Fundamental Research Funds for the Central Universities[3082015]; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2011FB02] ; Fundamental Research Funds for the Central Universities[3082015]; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2011FB02] ; Fundamental Research Funds for the Central Universities[3082015]; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2011FB02] ; Fundamental Research Funds for the Central Universities[3082015]; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2011FB02] |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Parasitology |
| WOS Subject | Parasitology |
| WOS ID | WOS:000303081500010 |
| WOS Keyword | SNAIL BIOMPHALARIA-GLABRATA ; SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; GLABRATA/ECHINOSTOMA-CAPRONI MODEL ; ECHINOSTOMA-PARAENSEI ; MANSONI INFECTION ; HEMOCYTES ; RESISTANT ; PROTEINS ; COMPATIBILITY ; PARASITES |
| Funding Organization | Fundamental Research Funds for the Central Universities[3082015]; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2011FB02] ; Fundamental Research Funds for the Central Universities[3082015]; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2011FB02] ; Fundamental Research Funds for the Central Universities[3082015]; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2011FB02] ; Fundamental Research Funds for the Central Universities[3082015]; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2011FB02] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.ihb.ac.cn/handle/342005/16941 |
| Collection | 鱼类生物学及渔业生物技术研究中心_期刊论文 |
| Corresponding Author | Nie, P (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China. |
| Affiliation | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China 2.Wuhan Univ, Sch Basic Med Sci, Wuhan 430071, Hubei Province, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Hao,Zhao, Qin Ping,Nie, Pin,et al. Identification of differentially expressed genes in Oncomelania hupensis chronically infected with Schistosoma japonicum[J]. EXPERIMENTAL PARASITOLOGY,2012,130(4):374-383. |
| APA | Wang, Hao,Zhao, Qin Ping,Nie, Pin,Sen Jiang, Ming,Song, Jian,&Nie, P .(2012).Identification of differentially expressed genes in Oncomelania hupensis chronically infected with Schistosoma japonicum.EXPERIMENTAL PARASITOLOGY,130(4),374-383. |
| MLA | Wang, Hao,et al."Identification of differentially expressed genes in Oncomelania hupensis chronically infected with Schistosoma japonicum".EXPERIMENTAL PARASITOLOGY 130.4(2012):374-383. |
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