中国科学院水生生物研究所机构知识库
Advanced  
IHB OpenIR  > 鱼类生物学及渔业生物技术研究中心  > 期刊论文
题名: Identification of differentially expressed genes in Oncomelania hupensis chronically infected with Schistosoma japonicum
作者: Wang, Hao2; Zhao, Qin Ping1, 2; Nie, Pin1; Sen Jiang, Ming2; Song, Jian2
通讯作者: Nie, P (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China.
关键词: Oncomelania hupensis ; Suppression subtractive hybridization ; Schistosoma japonicum ; Chronic infection ; Schistosome-snail interaction ; Head-foot
刊名: EXPERIMENTAL PARASITOLOGY
发表日期: 2012-04-01
DOI: 10.1016/j.exppara.2012.02.004
卷: 130, 期:4, 页:374-383
收录类别: SCI
文章类型: Article
部门归属: [Zhao, Qin Ping; Nie, Pin] Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China; [Wang, Hao; Zhao, Qin Ping; Sen Jiang, Ming; Song, Jian] Wuhan Univ, Sch Basic Med Sci, Wuhan 430071, Hubei Province, Peoples R China
WOS标题词: Science & Technology ; Life Sciences & Biomedicine
资助者: Fundamental Research Funds for the Central Universities[3082015]; State Key Laboratory of Freshwater Ecology and Biotechnology, Chinese Academy of Sciences[2011FB02]
类目[WOS]: Parasitology
研究领域[WOS]: Parasitology
摘要: Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum. The schistosome-snail interaction is biomedically important. To identify differentially expressed transcripts in O. hupensis chronically infected with S. japonicum, suppression subtractive hybridization (SSH) was used to construct a cDNA library in each direction for transcripts that are more abundantly enriched in head-foot part of the infected O. hupensis and for those that are more abundantly enriched in the uninfected, as head-foot part contains hemocytes and hemolymph which are associated with the snail internal defense system. After differential screening, 39 transcripts were identified, including nine and 30 transcripts enriched in infected and uninfected snails, respectively. Some of the transcripts have similar homology to available sequences in current databases, including transposase, caveolin-like protein, pancreatic trypsin inhibitor-like protein, prosaposin, glutathione s-transferase (GST), and several hypothetical proteins, while most of the transcripts do not match with any sequences in available databases. The identified transcripts were involved functionally in cell growth, metabolism, signal transduction, and immune responses. Two forward library transcripts and 11 reverse library transcripts were selected for real-time PCR, and 10 of them were confirmed to be consistent with the SSH results. It is intriguing to continue functional studies for some genes such as pancreatic trypsin inhibitor; a hypothetical protein (HS576367) related to calcium ion binding; GST; and several unknown proteins (HS576353 and HS576355). These identified differentially expressed genes may be key targets for understanding the molecular mechanism of co-existence during which the snail is unable to rid itself of the schistosome in chronic infection stage. (C) 2012 Elsevier Inc. All rights reserved.
英文摘要: Oncomelania hupensis is the unique intermediate host of Schistosoma japonicum. The schistosome-snail interaction is biomedically important. To identify differentially expressed transcripts in O. hupensis chronically infected with S. japonicum, suppression subtractive hybridization (SSH) was used to construct a cDNA library in each direction for transcripts that are more abundantly enriched in head-foot part of the infected O. hupensis and for those that are more abundantly enriched in the uninfected, as head-foot part contains hemocytes and hemolymph which are associated with the snail internal defense system. After differential screening, 39 transcripts were identified, including nine and 30 transcripts enriched in infected and uninfected snails, respectively. Some of the transcripts have similar homology to available sequences in current databases, including transposase, caveolin-like protein, pancreatic trypsin inhibitor-like protein, prosaposin, glutathione s-transferase (GST), and several hypothetical proteins, while most of the transcripts do not match with any sequences in available databases. The identified transcripts were involved functionally in cell growth, metabolism, signal transduction, and immune responses. Two forward library transcripts and 11 reverse library transcripts were selected for real-time PCR, and 10 of them were confirmed to be consistent with the SSH results. It is intriguing to continue functional studies for some genes such as pancreatic trypsin inhibitor; a hypothetical protein (HS576367) related to calcium ion binding; GST; and several unknown proteins (HS576353 and HS576355). These identified differentially expressed genes may be key targets for understanding the molecular mechanism of co-existence during which the snail is unable to rid itself of the schistosome in chronic infection stage. (C) 2012 Elsevier Inc. All rights reserved.
关键词[WOS]: SNAIL BIOMPHALARIA-GLABRATA ; SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; GLABRATA/ECHINOSTOMA-CAPRONI MODEL ; ECHINOSTOMA-PARAENSEI ; MANSONI INFECTION ; HEMOCYTES ; RESISTANT ; PROTEINS ; COMPATIBILITY ; PARASITES
语种: 英语
WOS记录号: WOS:000303081500010
ISSN号: 0014-4894
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.ihb.ac.cn/handle/342005/16941
Appears in Collections:鱼类生物学及渔业生物技术研究中心_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
Identification of differentially expressed genes in Oncomelania hupensis chronically infected with Schistosoma japonicum.pdf(480KB)----开放获取--View Download

作者单位: 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China
2.Wuhan Univ, Sch Basic Med Sci, Wuhan 430071, Hubei Province, Peoples R China

Recommended Citation:
Wang, Hao; Zhao, Qin Ping; Nie, Pin; Sen Jiang, Ming; Song, Jian.Identification of differentially expressed genes in Oncomelania hupensis chronically infected with Schistosoma japonicum,EXPERIMENTAL PARASITOLOGY,2012,130(4):374-383
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Hao]'s Articles
[Zhao, Qin Ping]'s Articles
[Nie, Pin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Hao]‘s Articles
[Zhao, Qin Ping]‘s Articles
[Nie, Pin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: Identification of differentially expressed genes in Oncomelania hupensis chronically infected with Schistosoma japonicum.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2016  中国科学院水生生物研究所 - Feedback
Powered by CSpace